We narrowed to 621 results for: Emb

1. The MTA at Addgene

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   ...transferring!    Many thanks to our Tech Transfer team members Maura Coughlin, Darcy Morse, and Matt Takvorian...

2. K. phaffii: Rising to the Occasion

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   ...proteins. Peroxisome studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain...

3. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   ...polling feature or the chat box. One key thing to remember regardless of the type of session you’re presenting...

4. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   ...is a science writer, former scientist, and proud member of Crowdfight. He is currently based in Spain and...

5. Networking Follow-up: I Met a Person. Now What?

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   ...Kamen's LinkedIn network! The connections between members of her network give rise to a starfish shape. ...

6. Scientists Map the SARS-CoV-2-Human Interaction Network

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   ...shipped out of the Krogan lab at UCSF. Our team members (shout out to Margaret Soucheray, Jeff Guo, Paddy...

7. What Good Citizenship Can Do for Reproducibility in Science

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   ...including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from direct editorial work...

8. Summer SciComm Series: A PhD in Science Communication

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   ...actually he drives better after a few drinks. A family member approached me for advice on her baby’s health but...

9. DAP arrays for multiplex gene editing

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   ...Typically, a single array encoding the tandemly assembled tRNA-gRNAs is placed downstream of a lengthy promoter...

10. Plasmids 101: NGS Quality Control for Pooled Libraries

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    ...libraries. Here are the key points you’ll need to remember when undertaking library amplification and sequencing...

11. Viral Vectors 101: Inverted Terminal Repeats

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    ...ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes be removed ...

12. Trading Spaces - Visiting Scientist Edition

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    ...trainee in your new lab, so make a good impression! Remember that your host is going out of their way to help...

13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    ...investigating DNA repair pathways.     Additonal Corn Lab Members who worked on this publication:         References...

14. Synthetic Photobiology: Optogenetics for E. coli

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    ...other plasmid-based systems, which will enable the assembly of larger systems. The work of Schmidl et al. ...

15. Resume Writing for Non-academic Science Careers

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    ...skills, and the job you are applying for. Also remember that a resume is typically no more than 2 pages...

16. Fluorescent Proteins 101: Luciferase

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    ...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

17. Advice for Scientists Starting a Lab

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    ...scientists to their labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early ...

18. Why and how to start studying chromatin (with Addgene's help)

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    ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

19. Viral Vectors 101: Viral Applications

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    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

20. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

21. Antibodies 101: Isotypes

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    ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

22. Improved Plasmid Maps Powered by SnapGene

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    ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

23. Luminescent Imaging with Nano-lanterns

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    ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

24. Multiplex Genome Editing with CRISPR-Cpf1

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    ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

25. Screening for Successful Genome Editing with Digital PCR

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    ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

26. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

27. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

28. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

29. Hot Plasmids - February 2022

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    ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

30. Sweating the Small Stuff: Details in the Lab

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    ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

31. CRISPR 101: Non-Homologous End Joining

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    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

32. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

33. Addgene: The First Twenty Years

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    ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

34. Viral Vectors 101: An Introduction to AAV

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    ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

35. The time and cost required to make a plasmid

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    ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

36. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

37. Choosing the B(right)est Fluorescent Protein: Photostability

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    ...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...

38. Summer SciComm: Preprints

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    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

39. Antibodies 101: Affinity Reagents

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    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

40. Transferable Skills Guide: Problem Solving

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    ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...