We narrowed to 642 results for: Emb

... are everything and you want to leave people remembering your potential as an entrepreneur, not your credentials...

...of interest into the FP-SEC vector using Gibson assembly. This construct will serve as the repair template...

...J. et al. Designing and using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797...

...pairs bridge the presynaptic and postsynaptic membranes at neuronal synapses. Image credit: IPI Browse...

...the necessary components of the viral genome to assemble the viral capsids (packaging and envelope plasmids...

...libraries. Here are the key points you’ll need to remember when undertaking library amplification and sequencing...

...including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from direct editorial work...

...transferring!    Many thanks to our Tech Transfer team members Maura Coughlin, Darcy Morse, and Matt Takvorian...

...Kamen's LinkedIn network! The connections between members of her network give rise to a starfish shape. ...

...polling feature or the chat box. One key thing to remember regardless of the type of session you’re presenting...

...trainee in your new lab, so make a good impression! Remember that your host is going out of their way to help...

...proteins. Peroxisome studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain...

...actually he drives better after a few drinks. A family member approached me for advice on her baby’s health but...

...Typically, a single array encoding the tandemly assembled tRNA-gRNAs is placed downstream of a lengthy promoter...

...is a science writer, former scientist, and proud member of Crowdfight. He is currently based in Spain and...

...shipped out of the Krogan lab at UCSF. Our team members (shout out to Margaret Soucheray, Jeff Guo, Paddy...

...investigating DNA repair pathways.     Additonal Corn Lab Members who worked on this publication:         References...

...other plasmid-based systems, which will enable the assembly of larger systems. The work of Schmidl et al. ...

...skills, and the job you are applying for. Also remember that a resume is typically no more than 2 pages...

...scientists to their labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early ...

...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

...ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes be removed ...

...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

...start getting results from your AAV experiments, remember to share your protocols and results through Addgene...

...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...

...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...