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We narrowed to 849 results for: HA

Showing: 481 - 520 of 849 results

  1. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... editors be close at hand? Once again, this post has a lot of acronyms, so we've included a glossary at...or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with... abasic site. The same protein (or its homologs) has more than one name. Indel Insertion/deletion A ...

  2. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...specific knock-out edits in a gene(s) of interest and has an average off-target frequency of 5%. Your experiment...Kostman. The most commonly used Cas, spCas9, has gone through multiple rounds of optimization which...where only DNA sites bound by Cas are sequenced, has been tested with some success. The major con is that...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...time Since the publication of our paper, our group has been inundated with requests to share our plasmids...). Also, we’ve got to mention that our FedEx guy has been the best: Thanks, Todd! As you can imagine, ...

  6. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...bind DNA targets without changing them. Thus, it has been widely adapted for a wide variety of applications...inhibition, among others. Over the past few years, dCas9 has also become a robust visualization tool to study ...

  7. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...supernatant during the production phase. However, this has not been well characterized as it is not a common...microvesicles as a vehicle for drug delivery that has recently gained interest special contexts, such as...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that’s...BioRender.com. The initial version of EvolvR has an editing window of ~17 nt from the nick site, which...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...yeast that same day.  The field of synthetic biology has led to advances in biofuel generation, green chemistry...

  11. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...with the indicated function. One huge reason CRISPR has become such a popular genome editing tool is its ...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer ...

  12. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...means persuading an employer that your past work has prepared you to contribute at a high level to their...rewarding. A truly passionate community of scientists has formed around science communication, including the...

  13. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...science writer Ed Yong’s book “I Contain Multitudes” has me considering the microbiome more often than not...these tools and to tell us how useful the service has been for their experiments. This helped reinforce...

  14. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...sfGFP to create SF-iGluSnFR. This improved variant has higher expression levels in bacteria and produces...ordered directly from Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems...

  15. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...neurons, directs high-level transgene expression, and has retrograde functionality to monitor and map neurons...doors in neuroscience. The retrograde serotype also has promising therapeutic applications; it can enhance...

  16. Hot Plasmids - January 2023

    Type
    Blog Post
    ...targeting, where siRNAs are ineffective and Cas13 has steep off-target costs. Additionally, this system...produced at Addgene and, as you might suspect, it has been one of our most commonly requested antibodies...

  17. A Deep Dive into BioBuilder

    Type
    Blog Post
    ... receiving training modules in synthetic biology has grown into a recognized name in the field of STEM...logic gates. This BioBuilder-Caltech collaboration has resulted in a new BioBuilder book chapter and an ...

  18. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification.... Using the same example plasmid as above (which has the XbaI site blocked by methylation), the figure...

  20. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...reagents. F1000Research, among many other journals, has joined a pilot project from the Neuroscience Information... up their own minds on whether the data analysis has been of good quality and whether they agree with ...

  21. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...and the master plasmid in one step. The kit also has other unique aspects that make it especially well-suited...for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be more efficient...

  32. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Singer’s lab at Albert Einstein College of Medicine has recently published techniques to follow translation...Rojas! Eugenia Rojas, PhD is a neuroscientist who has always been deeply interested in neurobiology.  She...

  33. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ... amounts or if the conjugated signaling molecule has a weak signal. For flow cytometry, secondary antibodies...introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal strength...

  34. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ... - typically within 2-4 months. After the animal has the desired level of antibodies in its bloodstream...their batch to batch variability. Once an animal has undergone a terminal bleed to acquire all of its ...

  35. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine...been approved by the FDA, and this mode of delivery has a lot of clinical promise moving forward. What viruses...

  36. All in a Twist: dsRNA

    Type
    Blog Post
    ...immune response machinery of the host. This pathway has been clinically exploited for antiviral purposes ...deplete expression of proteins of interest. RNAi has even made it into agriculture as species-specific...

  37. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus packaging...components found within the backbone. For example: pLXSN has the wild type LTR, an MCS for cloning X gene, an ...

  39. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ... human suffering and death. Doing research right has the potential to save lives and money. A 2014 analysis...physiologic or pathologic processes. Much progress has been made in this area in recent years, and you can...

  40. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor...allosteric inhibitors at Novartis. The ClonTracer library has been distributed all over the world and was recently...
Showing: 481 - 520 of 849 results