We narrowed to 877 results for: HA

...the opsin you use); the efficacy of optogenetics has already been proven. Therefore, a concrete, well ...scientists who pioneered the development of optogenetics, has genetically manipulated a number of the opsin genes...kinetics of opsin activity/inactivity. Dr. Deisseroth has also generously made many of his optogenetic constructs...stereotaxic surgery (see videos below). The Deisseroth lab has developed many of these AAV constructs with various...

... and allows targeting of specific cell types, it has a limited capacity to carry large DNA cargos. As ..., the search for smaller, compact genome editors has become a key focus in recent years. TnpB nucleases...nuclease domains (HNH and RuvC-like), and Cas12a has one (RuvC-like), TnpB also operates with a single...

... newest kid on the block in the sequencing world has been long-read sequencing. Whereas the individual...auto-alignment, plus additional automation in the backend, has enabled the QC to keep up with the pace of deposits...plasmid. By working with the Data team, the QC team has also improved efficiency in many of their methods...

...stringently controlled and has a low copy number. Like the E. coli chromosome, it has a single ori and requires...Once the average copy number for a given plasmid has been reached, negative feedback circuits reduce replication...

...RNA and can express fluorescent proteins. This lab has created an automated tank for feeding and maintenance...scolopes (Joshua Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually... injection (hard to do, expensive, not every lab has the capability) with receptor mediated endocytosis...

... improve its stability in vivo. However, Spinach has proven useful in the engineering of riboswitches....syndrome (Strack et al., 2013). Since then, Spinach2 has been used to investigate intracellular viral DNA ...better choice for imaging than the monomer. Broccoli has been developed into a split aptamer that can be used...

...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

... have been spent within a lovable community that has allowed me to expand my knowledge more than ever ... had opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want...

...emotions, and even what therapeutic properties it has. As with other works of Dr. Sacks, Musicophilia includes... pretty biased as the founder, but our book club has become one of my favorite parts of Addgene’s culture...

... an assistant professor at Kyushu University. He has a particular interest in the development of sensory...Imai is a professor at Kyushu University. His lab has been studying functional and anatomical aspects of...

...requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify...in human pluripotent stem cells, a cell type that has been difficult to modify in the past.   Figure...

...depositing lab. If your vector is large (>10 kb) or has a low copy number, try following protocols for low...easiest and clearest ways to determine if your sample has recombined. Choose a restriction enzyme that will...

... A practical guide by Paula J. Silvia  This book has tips for how to write a lot as an academic. If you...your Mendeley library in Microsoft Word. Mendeley has a web and desktop version that works with Windows...

...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...

...cells, but since the majority of the viral genome has been swapped for alternative genetic cargo, they ... there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for...

... to objects that exist online, but this approach has been adapted for the identification of physical materials...Let’s take a look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier...

...TOPO-TA) cloning; however, the TOPO cloning technique has also be adapted for blunt end cloning. So how does...PCR products. Keep in mind that Taq polymerase has an error rate of about 1 in 3,500 bases. Typically...

...library screen; instead I thought they would focus on “Has this person been consistent throughout their career...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ...

...prokaryotes; the ultimate victor will be the species who has the strongest will to survive. Genome engineering...biological and translative technologies. Addgene has empowered researchers to harness previous experimental...

...restriction enzyme based cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...

...personalized genetic medicine (Musunuru et al., 2025). Much has been made of the speed with which KJ’s successful...acceptable in every case, however, and recent work has already been published on improving base editing ...R. M., Pacesa, M., Smith, B. K., Christie, K. A., Ha, L. L., Meis, R. J., Clark, A. B., Spinner, A. D....

...goodie — a phrase used to refer to something that has fallen out of fashion but is still useful and most..._block %}   Interfering in the future While RNAi has retreated a bit to the background, it remains a solid..., Fort, A., Gebhard, C., Goldowitz, D., Guhl, S., Ha, T. J., . . . De Hoon, M. J. L. (2017). An integrated...

... editors be close at hand? Once again, this post has a lot of acronyms, so we've included a glossary at...or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with... abasic site. The same protein (or its homologs) has more than one name. Indel Insertion/deletion A ...

...with the macromolecules it is added to. Biotin also has an exposed side chain that can be easily manipulated...coated with avidin. The biotin-avidin interaction has also been adapted for use in immunohistochemistry... either the N or C terminus of the protein. BirA has been shown to effectively biotinylate AviTag expressing...

...specific knock-out edits in a gene(s) of interest and has an average off-target frequency of 5%. Your experiment...Kostman. The most commonly used Cas, spCas9, has gone through multiple rounds of optimization which...where only DNA sites bound by Cas are sequenced, has been tested with some success. The major con is that...

...localized, rapid and reversible microtubule severing and has very low dark-state activity and toxicity. All of...could be extended to other GTPases. The Chow Lab has also generated a variety of membrane-recruitment ...engineering other enzymes. Conclusion We hope this post has illuminated some of the many tools available for ...

... beta-barrel structure. Although the beta barrel has no strong affinity for other proteins, it may still...modifications. Despite these potential issues, GFP has been used successfully in countless fusion proteins...localization of critical fusions (e.g. tubulin or connexin) has been used as a proxy for dimerization (Shaner et ...

...track single cell dynamics at the organism level has been made possible by tools that allow cells to become... such as neurons in the fly visual system, which has been difficult to study previously (Hadjieconomou...of cells in the intestinal crypt, and since then has become a powerful tool for understanding cell proliferation...

... receiving training modules in synthetic biology has grown into a recognized name in the field of STEM...logic gates. This BioBuilder-Caltech collaboration has resulted in a new BioBuilder book chapter and an ...

...HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...yeast that same day.  The field of synthetic biology has led to advances in biofuel generation, green chemistry...

... chosen promoter, and Bloomington’s Stock Center has fly lines expressing Gal4 under a variety of promoters...finishing his immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila...

...with the indicated function. One huge reason CRISPR has become such a popular genome editing tool is its ...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer ...

...means persuading an employer that your past work has prepared you to contribute at a high level to their...rewarding. A truly passionate community of scientists has formed around science communication, including the...

...neurons, directs high-level transgene expression, and has retrograde functionality to monitor and map neurons...doors in neuroscience. The retrograde serotype also has promising therapeutic applications; it can enhance...

...targeting, where siRNAs are ineffective and Cas13 has steep off-target costs. Additionally, this system...produced at Addgene and, as you might suspect, it has been one of our most commonly requested antibodies...

... size distribution and concentration of ApoB-LPs has been shown to have a strong correlation with cardiovascular...indicators difficult to work with.  The Gottschalk lab has surveyed a range of microbial rhodopsin GEVIs in ...

...bind DNA targets without changing them. Thus, it has been widely adapted for a wide variety of applications...inhibition, among others. Over the past few years, dCas9 has also become a robust visualization tool to study ...

...supernatant during the production phase. However, this has not been well characterized as it is not a common...microvesicles as a vehicle for drug delivery that has recently gained interest special contexts, such as...

...used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that’s...BioRender.com. The initial version of EvolvR has an editing window of ~17 nt from the nick site, which...

...sfGFP to create SF-iGluSnFR. This improved variant has higher expression levels in bacteria and produces...ordered directly from Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems...