We narrowed to 809 results for: MAK

...Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and what can they...excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling particularly with...

...backbone and differ only in a small region. This makes them useful for a wide variety of high-throughput...specific cells — as well as their descendants. This makes them useful for lineage tracing, among other applications...

...from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward...that with this award from Addgene, we’ll be able to make big progress in elucidating association between ...

...?” and "What type of science careers would would make a good fit for me?" Whatever would I do next and...for sales and marketing jobs Like most choices we make, my job choices were mostly by chance. Luckily, ...

...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety...engineer yeast to do similar things. The focus is to make yeast engineering accessible to everyone but these...

...Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-...restriction enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’...

...published in the same journals as those that did make deposits to Addgene.  Insomuch as different journals... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

...tools out there to visualize genomic loci, what makes CRISPR-Sirius unique? Unlike fluorescent in situ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

...changes) the original identifier loses its meaning, making it difficult or impossible for researchers to track...verified and identifiable to save researchers time and make their work more reproducible. While Addgene plasmid...

...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...different constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that ...

...of their optimally suited temperature ranges and make them grow better at suboptimal ones, which could...within a regular mesophilic host system. This would make the host able to grow at low temperatures suitable...

...     The Trimmer Lab has continued to work to make the sequences of monoclonal antibodies publicly ...sequences from the Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded...

...’s a good idea to make sure that laser is available in your machine before you make your purchase. Picking...

...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify... are a few drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...

...terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV ...and disruptive mutations and truncations that can make vector propagation and sequencing a headache. How...

...then inserted into the host's genome and is used to make more virus. The most studied (and used in the lab...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

...Sequence- and ligation-independent cloning: A SLIC makeover In 2007, LIC received an important update, courtesy...for multi-fragment DNA assembly, and its low cost makes it ideal for researchers doing large amounts of ...

...control for a specific marker in your experiment. To make an FMO sample, you take a subset of your experimental... is prepared very similarly to a FMO sample: you make a sample from a combined subset of your experimental...

... Thus, the adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic ...immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

... experiments and it’s already looking like it’s making a big difference. We can now look at many more ...out there. Addgene: Why was it important to you to make these plasmids available to other scientists quickly...

...testing and a lack of well-characterized genetic tools make it challenging to engineer a specific function in...construct our color changing flower we are carefully making our way through the IP landscape, using plant parts...

...exposure to autophagy-inducing conditions. Rosella makes it easier to study the mechanisms behind autophagy... shown in Sargsyan et al. It is also possible to make Rosella fusions and thereby monitor autophagy of...

...However, they note that certain improvements could make CRISPRainbow even better. Adding an additional hairpin...activation/repression and genome editing. Ready to make your cells a little more colorful? CRISPRainbow ...

...comment online! Want the general public (say, policymakers or community organizations) to use your work...those plasmids alongside a journal article, but it makes the research materials available to the community...

...time to read the book.  Intuitively, this probably makes sense. It’s a lot easier to read a formulaic fiction...happens to us all!) and offer a variety of options to make your content more accessible to your audience.  ...

...short linker peptide. The lack of constant region makes them less immunogenic, but they have lower affinity...produced in bacteria and are generally cheaper to make than antibodies. Minibodies are created by binding...

...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

...opsin-expressing cells). For other things to keep in mind when making fluorescent protein fusions, check out Joachim ...Fluorescent Proteins 101: GFP Fusion Proteins – Making the Right Connection.     Thank you to our guest...

... she couldn’t run enough workshops by herself to make the biggest impact. When she spoke with Teytelman...visualization. Not everyone in the group was able to make it physically to instruct the workshop at the conference...

...promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand how...Witz, Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009...

...systemically administered in an organism. These features make AAV ideal for human therapies and in vivo laboratory...delivery of genetic cargo to a host cell. In order to make room for a gene of interest, AAVs used in research...

... been talking about Click Chemistry since 2001, making use of robust reactions that work in just about...particularly useful for a kind of cellular computation, making possible multiple logic-gated steps. Beyond the...

...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express...

...is no small task. As a scientist, it is useful to make literature searching and reading a regular part ...Keeping up with the literature There are a few ways to make sure you are keeping up with the literature in your...

...low-cost, field-based diagnostics, our approach could make an impact. Our motivation was underscored by the... switches and amplification primers. This helped make sure that we had a number of successful sensors ...

...safety profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are...homology directed repair (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated...

...transfer office or other authorized office directly to make sure the deposit agreement/MTA is taken care of ...spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries with...

...off of a circular plasmid via PCR. Typically, to make these constructs, developmental biologists have ...please write to [email protected] and if you make new plasmids for investigating different planarian...

.... The budding yeast, Saccharomyces. cerevisiae, makes a great reference organism for the project because...PubMed Central PMCID PMC232622. 6. Winzeler, D.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K.,...

...When it comes to making the genetic manipulation, there are two main decisions to make: 1. Which modification...