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We narrowed to 801 results for: MAK

Showing: 481 - 520 of 801 results

  1. Plasmid Cloning by PCR

    Type
    Blog Post
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

  3. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...?” and "What type of science careers would would make a good fit for me?" Whatever would I do next and...for sales and marketing jobs Like most choices we make, my job choices were mostly by chance. Luckily, ...

  4. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...published in the same journals as those that did make deposits to Addgene.  Insomuch as different journals... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...tools out there to visualize genomic loci, what makes CRISPR-Sirius unique? Unlike fluorescent in situ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

  7. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety...engineer yeast to do similar things. The focus is to make yeast engineering accessible to everyone but these...

  8. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV ...and disruptive mutations and truncations that can make vector propagation and sequencing a headache. How...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-...restriction enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’...

  11. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...control for a specific marker in your experiment. To make an FMO sample, you take a subset of your experimental... is prepared very similarly to a FMO sample: you make a sample from a combined subset of your experimental...

  12. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...then inserted into the host's genome and is used to make more virus. The most studied (and used in the lab...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

  13. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ... Thus, the adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic ...immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  15. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify... are a few drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...

  16. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...different constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that ...

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...     The Trimmer Lab has continued to work to make the sequences of monoclonal antibodies publicly ...sequences from the Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded...

  20. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...testing and a lack of well-characterized genetic tools make it challenging to engineer a specific function in...construct our color changing flower we are carefully making our way through the IP landscape, using plant parts...

  21. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...systemically administered in an organism. These features make AAV ideal for human therapies and in vivo laboratory...delivery of genetic cargo to a host cell. In order to make room for a gene of interest, AAVs used in research...

  22. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express...

  23. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...exposure to autophagy-inducing conditions. Rosella makes it easier to study the mechanisms behind autophagy... shown in Sargsyan et al. It is also possible to make Rosella fusions and thereby monitor autophagy of...

  24. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...However, they note that certain improvements could make CRISPRainbow even better. Adding an additional hairpin...activation/repression and genome editing. Ready to make your cells a little more colorful? CRISPRainbow ...

  25. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

  29. Summer SciComm: Preprints

    Type
    Blog Post
    ...comment online! Want the general public (say, policymakers or community organizations) to use your work...those plasmids alongside a journal article, but it makes the research materials available to the community...

  30. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...time to read the book.  Intuitively, this probably makes sense. It’s a lot easier to read a formulaic fiction...happens to us all!) and offer a variety of options to make your content more accessible to your audience.  ...

  31. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...short linker peptide. The lack of constant region makes them less immunogenic, but they have lower affinity...produced in bacteria and are generally cheaper to make than antibodies. Minibodies are created by binding...

  33. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... of MoClo. Image courtesy of Rob Hurt. What makes MoClo systems so powerful is their modularity — ...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...

  34. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...to CRISPR experiments, therefore, you often must make a choice about what is your top priority: location...trying to knock-in a patient mutation or need to make a precise amino acid change, there is very little...

  35. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...transfer office or other authorized office directly to make sure the deposit agreement/MTA is taken care of ...spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries with...

  36. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...receiver cells. There are over 40 trillion cells that make up the human body and G-baToN provides versibility...

  37. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...safety profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are...homology directed repair (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated...
Showing: 481 - 520 of 801 results