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We narrowed to 974 results for: lat

Showing: 481 - 520 of 974 results

  1. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...non-homologous end joining (NHEJ), HDR occurs at a relatively low frequency, especially in nondividing cells...the cell to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of...repairs the DNA using the U-containing strand as a template, copying the base edit. Figure 2: Cytidine...repair, thus removing these cells from the edited population.  Another way to improve base editing efficiency...improvement in editing efficiency (Koblan et al., 2018). Later directed evolution efforts yielded an editor with...While cytosine base editors often produce a mixed population of edits, ABEs do not display significant A to...

  2. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...in your favorite neuronal population and track connections to that population. Limitations of common genetically...to define those that target specific neuronal populations in the brain. The Allen Institute and the Janelia...also been important in mapping, monitoring, and manipulating neuronal circuits. Epitope tags are short antigenic...cells. They can be used for protein localization, isolation, and tracking experiments (Rizzo, 2009). With ...

  3. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ... to grow on plates or in liquid culture to amplify the library, followed by DNA isolation. The amplified...screening-scale transduction of cells, resulting in cell population mutated by the viral pooled library. These cells...payload is adequately represented in the cell population, you’ll need to infect many more cells than the...Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale...

  4. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...do a lot of DNA work but never get to actually manipulate the organism they study (unless if you count ...study of diverse organisms. Methods for genetic manipulation still need to be developed for each organism... currently for terrestrial frogs. Cnidarian-Dinoflagellate symbionts (Virginia Weis) Sea anemone Aiptasia...Henry) This black footed slipper snail can be manipulated by injected RNA and can express fluorescent proteins...is developing tools for pan-arthropod genetic manipulation. The ReMOT control system (Receptor-Mediated...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    ...PE6b was very efficient for short, unstructured templates and significantly reduced undesired edits (indels...complex edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing...potential to reduce negative side effects. This platform offers a promising approach for experimental therapies...illuminated with blue light, catalyzing the biotinylation of nearby proteins. B) TransitID uses sequential... et al. (2023). Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control...

  6. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...peripheral blood mononuclear cells (PBMCs). PBMCs isolated from whole blood present many benefits for the...patients are reprogrammed less efficiently, no such correlation has been found between the age of the patients... amplification in the host cell. This enables relatively high and long-term expression of the reprogramming...using PBMCs as the source of somatic cells. This translates to only about 16 hiPSC colonies per 100 ml of...Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells...

  7. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...Secretary of Labor Thomas Perez updated the overtime regulations of the FLSA. The exemption salary was set at ... postdocs are at each of these institutions (populations potentially from 1 to 5761, according to the ...research on these institutions, we can say with relative certainty that just over 40% of current postdocs...for academia Changes are afoot for the postdoc population, and it is unclear how this will all shake out...effects the new laws will have on the postdoc population. In the current funding climate combined with...

  8. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Having successfully navigated the trials and tribulations of the process, I’ve provided guidance and mentoring...interviews but no job offers, is a failure to clearly articulate one's talents and ideas. Some key points from...question? This should be something that anyone could relate to, that people would agree is important to understand...provide the first defined model system that we can manipulate to study pathology and therapies." This is...interview, your previous success will not necessarily translate into getting a job. Your papers and pedigree get...

  9. March for Science

    Type
    Blog Post
    ...can do and, in this way, science has always been related to government and, consequently, politics. More...Inclusion of data informing policy specifically related to the following subjects: Climate change Vaccines...Healthcare Science education To make scientists relatable (17, 20). This is an effort to show the general...all you need to do is select your state. State Legislatures: To find the website to contact representatives...Lawmakers need to use a scientific approach to formulating views Pew Research Center: Public and Scientists...

  10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...strategy for increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs ...acceptor of the transgene to help prevent the accumulation of such truncated proteins. Examples of use...homologous recombination approach delivers large donor templates using two sequential homology directed repairs... the gRNA target site that mediated the donor template’s integration so that the site is reconstituted...which results in the integration of a larger donor template.  Drawbacks: Transgene expression varies with...

  12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... 2,3-N-linked sialic acid4 AAV receptor2 Platelet-derived growth factor receptor (PDGFR) α and β3...and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish the capsids...-stock viral prep service.  AAV2 Despite being isolated second, AAV2 is the best studied and most commonly...our in-stock viral prep service.  AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency...retinal gene delivery from the vitreous. Science Translational Medicine, 5(189), 189ra76. https://doi.org/10.1126...

  13. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ..., generating new therapeutics, and sometimes formulating new biological concepts. However, rates of reproducibility... However, even simple procedures like pouring a plate or running a gel have intricacies that aren’t always...have plans to expand to other protein technologies later. Organizations such as BenchSci and FPbase are working...find information about research tools. BenchSci collates information about antibodies while FPbase curates... soon, the Center for Open Science does host a platform for pre-registration wherein researchers can submit...

  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene Expression in Nonhuman...primate. Likewise, in vitro efficacy doesn’t always translate to in vivo efficacy. You’ll therefore need to ... strong early gene expression, but can become methylated over time, resulting in decreased expression ...consider. First, the titer required is inversely related to transduction efficacy: the higher the efficiency...

  15. Finding Your Perfect Job After University

    Type
    Blog Post
    ...Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new ... felt I needed more practical lab experience to later gain a full time position in a lab. I worked on ...left me optimistic that I would find a genetics related role soon after finishing my MSc. The masters program...I had received all the training and experience relating to good manufacturing practice (GMP) and good ...and decided to compromise and applied anyway. I later found out the job was with Addgene. I had used plasmids...

  16. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...antigen-antibody interactions in a plate-based format, making it suitable for comparing relative affinities across mutants...structure of your antibody-antigen complex as a template. This structural framework then guides the specific...their large-scale recombinant antibody production platform. IPI is a leading expert on antibody discovery..., & Kruse, A. C. (2018). Yeast surface display platform for rapid discovery of conformationally selective...

  17. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...investigator. In basic science (as opposed to translational, biomedical, or applied sciences), people conduct...everything there is to know is a strange and exciting revelation. Science in any field can generally be applied...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...students, women, BIPOC, and other minoritized populations. Research universities often have scholarships...

  18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...broth and on the agar plates. Mycoplasma colonies are easily visible on the agar plates. This method is capable...detection, DNA staining, fluorescent tagging, agar plating, etc. Be wise to choose any of the techniques listed... (Uphoff and Drexler, 2011). Culturing on agar plates/broth is considered the ‘gold standard’ for detecting...prevent contamination rather than eradicating it later. Don’t be scared when mycoplasma infects your cell...

  19. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference...inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed repair (HDR) can be ...using a single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex...Candida albicans A new CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans...

  20. Science Communication for Everyone

    Type
    Blog Post
    ...like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate” could...different topics of science, especially disease related topics. Neurodegenerative and brain disorders are...illicit tampering” to general audiences while “manipulate” could mean “worked with” or “changed” to scientists...

  21. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...said. “It opens a new avenue of modifying and manipulating endogenous eukaryotic RNA.” Endless possibilities...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1...(1):7. doi: 10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview More...

  22. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...Expanding your network of relationships early and often is the most effective tactic a scientist in training...result of existing professional (and personal) relationships. However, most job seekers will and should apply...after the academic bench. Utilizing existing relationships as part of the job application process can dramatically...you can use alumni databases, friends, and even relatives to network your way to a hiring manager. Learn...

  23. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...let you know before these releases happen (and congratulate you on your paper!). And as always, we won’t.... But this way, as soon as we become aware the related journal article has been published we’ll get the...the plasmids’ citation when the paper comes out later. Or, if a project isn’t working out as planned, ...citation to the final journal article version any time later. We recognize that a preprint isn’t necessarily ...

  24. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...to detach from the constant stream of thoughts related to lab research and gain fresh perspectives. Such...encourages exploration of tangents or seemingly unrelated ideas. It's beneficial to carry a notebook at ... identify areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives...everything is a miracle.” This quote encourages us to contemplate our approach to life and how our viewpoints influence...

  25. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...by an inexpensive and widely available imaging platform: ultrasound. Challenges of in vivo imaging with...water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy. The Shapiro lab recently discovered...ultrasound imaging. Ultrasound is an attractive imaging platform for in vivo imaging experiments because  ultrasound...imaging to show where the bacteria localize in relation to internal organs. The limit of detection for...

  26. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... live animal that can be correlated to behavior, the technique is relatively slow and is not suited to...activated and inhibited neurons in live mice Report modulation of DA signaling during learning and reward consumption...Petzold, and Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its...

  27. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers to silence...neuron more positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

  28. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification, and ...a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without...agrochemical compounds that have the potential to regulate the parasitization of cereal crops. As a result...activity on NAG PAMS and reduced activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout...

  29. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    [email protected] Congratulations to the Golden Stopwatch awardees! Congratulations to all of the winners...faster with technology transfer offices (TTOs). The latest of these efforts is the Golden Stopwatch Award ...Team! Why MTAs Matter Though they may seem like boilerplate drudgery to some scientists, MTAs are important—they...

  30. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ..., increasing the impact of the work, and as a collateral benefit, increasing the impact of the specific...comments to “you” I can easily check if I would have formulated specific comment differently if I would be speaking...Root System Architecture of Arabidopsis HapMap population in Amsterdam. Currently, she is a postdoc at ...expression patterns in plants with enhanced sodium accumulation in their roots and (3) development of tools ...

  31. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling and structure-guided...which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and emission...combination results in a 2.6-fold increase in signal relative to mCerulean imaged with a standard CFP set. mTFP1...fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID...

  32. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...multiple, small colonies After streaking LB agar plates, you may notice that there are both large and small... bacterial cultures with lentiviral vectors, the flat white colonies contained the full plasmid, whereas...preps. In some cases, we suggest gel extraction to isolate the full, intact plasmid. Please see our gel extraction...recombination of plasmid DNA without affecting mRNA translation efficiency or half-life. RNA 25:507–518. https...

  33. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ... encode spikes found on all of the different circulating variants. This allowed us to create pseudoviruses... the vaccine, we've been able to measure their relative ability to neutralize.”   Figure 2: Sera... shocked [with these results] because we were simulating the live virus experiment,” says Balazs. “But...resources on the Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources...

  34. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several... neuroscience was carried over and allowed to percolate within a new intellectual environment. Over time...perception, scientific uncertainty and precautionary regulation was all necessary to inform the experimental ... health policy implications arising from sport-related concussion.   More resources on the Addgene blog...

  35. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...Careers has developed a web-based, career-planning platform called the Individual Development Plan (IDP) and...using multidisciplinary, collaborative, and translational approaches for scientific tool and technique... of the project. For example, how can future regulations impact your work? What design variables need ...Patent process Business Models Communication Regulatory knowledge Networking Blogging Writing...

  36. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...thought there was room for improvement and a year later it became quite clear that others thought the same... piñata burst, value was subjectively ascribed relative to the basic needs of each individual; the  community...newer tools may still prove somewhat useful for the latest NTT targeting Cas protein, Cpf1. Separately, on-target...tools allow the user to save the results and return later or share them. Community exclusivity: Different...sites, scores and the statistics that go into calculating them. For this, some online tools such as Cas-OFFinder...

  37. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...these small DNA strands can form concatemers in later steps. This new NEB kit includes a step that both...the DNA and prevents it from forming concatemers later. The "FS" stands for Fragmentation System and this... an NEB drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the...understand? How does the information I’ll be learning relate to me as an individual and Addgene as a company...

  38. Hot Plasmids - January 2023

    Type
    Blog Post
    ...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...area, as the image below shows:    This system ablates tumors just as effectively as standard CAR-T cells...epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to only having...tested hfYFP in expansion microscopy as well as correlative light and electron microscopy and observed high...

  39. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... arrangements may affect nuclear processes. The latest of these visualization tools is CRISPR-Sirius, ...specific loci based on the guide RNA sequence. A year later, they developed CRISPRainbow (Ma et al., 2016), ...chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow labeling... proteins. (B) CRISPR-Sirius used to visualize relative locations of the subtelomeric region of the pericentromeric...

  40. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... are trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al., 2022...therapeutic antibody itself or as impurities in the formulation. Efforts to predict T cell epitopes focus on ..., and can be used as a whole or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes...
Showing: 481 - 520 of 974 results