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  3. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...when budgets are constrained. This collaborative approach eliminates redundant work, saving time and allowing...be listed as direct costs from a CRO in grant applications, addressing the new funding structures and indirect...

  4. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ... shipped! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years! We're ... as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently...

  5. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...talks, symposiums, poster sessions, exhibit booths, happy hour socials, and late, late night socials. As a...depositing scientists at #SfN14. And we've been so happy to hear that we've been able to help their labs ...

  6. Addgene's Website Update

    Type
    Blog Post
    ...: The Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as ...through the yellow popup at the bottom of the page. Happy surfing!   This post was written by Addgenies Jo...

  9. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...than longer ones. Thus, you can determine the approximate length of a DNA fragment by running it on an ... bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock... bands will be differentially intense. If this happens, you can just soak the gel in EtBr solution and...Run the gel at 80-150 V until the dye line is approximately 75-80% of the way down the gel. A typical run...are usually referred to as ‘bands’ due to their appearance on the gel. Pro-Tip If you will be purifying ...

  13. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...microcentrifuge. Remove the gel from the plastic wrapper and rinse with deionized water. Gently remove the...5 µL of the prestained protein ladder to the appropriate well. Pro-Tip When possible, skip one lane before...µL of each recombinant antibody sample to the appropriate well. Pro-Tip Leaving clear lanes between samples... Take a brightfield image of the gel with an appropriate imaging system. Figure 1 Recombinant antibody...Gels . Select Plot Lanes . One graph per lane will appear with peaks representing each protein band. Use ...

  14. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...

  16. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...emphasizes that people should “follow their passions, be happy, and match their personal goals to their career ...analytical and critical thinking skills that are applicable to a variety of careers outside of academia. ...

  17. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ... at least we can reduce the rate at which that happens and reduce the manufacturing of new containers,...bin or box in your tissue culture room for the wrappers and ship them back. Start a glove recycling ...

  18. Fostering communication in remote collaborations

    Type
    Blog Post
    ...willingness of the team to adopt various tools, this may happen over email or using rapid communication tools such...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the...

  20. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...individualized optimizations, safety testing, and approval. In contrast, a system that can deliver a healthy...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...

  21. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However...antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions prevents FOP bone...

  22. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...managers and some take on leadership roles. You may be happy with a primary role of doer for your entire career...they miss the hands-on bench work. To really be happy, you may need to find a way to do, manage and lead...

  23. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...respond to the employee’s level of discomfort appropriately to get the message across. Listen to our podcast...to you. What are We Learning: What would have happened if _________? What would have been a better outcome...

  25. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline...

  30. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...References Biddy, Brent A., et al. "Single-cell mapping of lineage and identity in direct reprogramming...

  31. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...

  32. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ... work.  And while these pieces aren't always appropriate for your formal scientific communications, they...still get invested in the journey? The same thing applies for science storytelling. You can introduce your...

  33. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...categories: ones induced by the researcher and ones that happen naturally.  Sample Denaturing  First, let’s think...SDS-page gel. As the protein unfolds, new epitopes may appear, while others may get hidden. Monoclonal antibodies...

  36. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools...

  37. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis...

  40. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...

  41. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill...

  42. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...for additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be ...AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways...

  43. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...science. It's a series of case studies in what happens when scientists bring biases to research and don't...English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day...

  44. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies...

  46. The MTA at Addgene

    Type
    Blog Post
    ...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...and our MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer...

  48. How to Lead a Great Meeting

    Type
    Blog Post
    ...assign each one using group members’ initials. If appropriate, email this around after the meeting. Accountability...Remember, many of the more in-depth discussions can happen “off-line” with just one or two relevant people...

  49. Components of CRISPR/Cas9

    Type
    Blog Post
    ...gRNA) and their prefered Cas9 variant (with the appropriate PAM sequence) to modify the desired genomic locus... more to be found in the full CRISPR 101 eBook. Happy Reading! ...

  50. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications.   References Refactoring and optimization ...
Showing: 601 - 650 of 954 results