Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 784 results for: SCR;

Showing: 601 - 650 of 784 results

  12. The Challenges of Cell Culture

    Type
    Blog Post
    ...free of contamination and match their original description. The oldest, largest, and most diverse of these...

  13. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...

  22. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice...

  24. March for Science

    Type
    Blog Post
    ...freeze all grants, contracts The Guardian: Trump to scrap Nasa climate research in crackdown on “politicized...

  31. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...differences. If you are performing CRISPR library screens, the same principle holds true - the higher the...

  41. CRISPR Guide

    Type
    Collection
    ...integration of donor DNA. Genome-Wide Screens Using CRISPR In a genetic screen, a population of cells with mutations...Targeting dCas9 to transcription start sites is sufficient to repress transcription by blocking initiation..., G. M. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for...in your lab, from knockouts to pooled library screening. Science...CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9 Alternatives Resources ...approaches like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...technologies, making CRISPR perfect for genome-wide screens. This guide will provide a basic understanding ...

  42. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...The collection can be used for screening transcription factors for cellular programming or cancer drug ...simplifies identification of the transcription factor isoform in pooled screens. This collection and library...library can be used for screening transcription factors for applications such as cellular programming or cancer...for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq (PDF, 59.2 KB)...to simplify identification during screening. Library ID PI Description MORF library 137000, 192821, 1000000218...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...

  43. Plan Your Experiment

    Type
    Collection
    ... interfere with transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa...fused to transcriptional activators that need to be within a given range of the transcription start site...validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment....Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a wider window than most...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different... interest, where the Cas9 protein can block transcription from ever initiating. gRNAs targeting regions...

  44. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Access to the Paper & Protocol Video Download the manuscript here: Bauer et al, 2014 322.2 KB If you have ...production of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization...referenced Tables and Figures, please access the manuscript PDF posted at the top of the page. CRISPR Design... the genomic recognition site. NOTE: Figure 1 describes possible deletion strategies for genes and non-coding...leading to nonsense mediated decay of the mRNA transcript ( Figure 1B ). Use the example guides for the...be 24-mer oligos ( Table 4 ). Design Deletion Screening Primers Design one set of primers internal to ...deletion. For this approach, the internal primers described in step 2.1 may be omitted. CRISPR Cloning Anneal...

  45. Luciferase Plasmid Collection

    Type
    Collection
    ...promoter/enhancer regions. Blue/white screening can be used for screening of transformants in cloning process...Optimized STARR-seq (Self-Transcribing Active Regulatory Region) : Genome-wide screening of enhancers using ... protein half-lives, making them useful as transcriptional reporters, but are less-than-ideal protein ...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion of...Vector for investigating regions controlling transcription Debrya Groskreutz 18760 MSCV IRES Luciferase...Vector for investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla...Vector for investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla ...

  46. Zhang Lab CRISPR Page

    Type
    Collection
    ...below. Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic...RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries...and mouse) for performing genome-wide knockout screens. The libraries are available in two formats: 1 ...) is an engineered protein complex for the transcriptional activation of endogenous genes. It consists...database (23,430 isoforms) for gain-of-function screening. For more information, please visit the Addgene... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with...

  47. Tetracycline Inducible Expression

    Type
    Collection
    ...Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator..., the TetR protein binds to tet O, blocking transcription of the downstream gene. If tet or one of its...to tetracycline and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a ...tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation domain); rtTA: reverse tetracycline-controlled...transactivator (fusion of mutated TetR with VP16 transcriptional activation domain). For simplicity, other promoter...to eliminate binding of endogenous mammalian transcription factors to further minimize background expression...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...

  48. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...: dCas9 fused to transcriptional activators or repressors can modulate transcription in a reversible manner...palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the... locus makes it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify ...are available for gene knockout, as well as transcriptional activation or repression. Combined with the...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...

  49. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...mutant cells that are then screened for a phenotype of interest. Screening experiments using a pooled ...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...Blog Posts CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing... gRNAs for each target gene. In a CRISPR screening experiment, target cells are treated with the ...libraries and keep the following in mind: CRISPR screening experiments require electroporation to amplify...

  50. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ... Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description Industry... Plasmid Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description Industry ...Industry Greens Plasmid Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description... Description Industry Do you have suggestions for other plasmids that should be added to this list? Fill...
Showing: 601 - 650 of 784 results