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  2. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV...use a dimmer fluorophore. However, these aren't strict rules and in practice the differences in brightness...

  4. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... nuclease function. Stem loops 2 and 3 are not strictly necessary for in vitro cleavage, but they improve...Figure 3 crystal structure, reflecting the less stringent recognition mechanism that can tolerate mismatches...

  5. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant...compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal ...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...chimeric offspring shows the ability of the iPSC to contribute to all three germ layers, and the ability of ...iPSCs to form functional germ cells. The highest stringency test for mouse iPSCs, tetraploid complementation...

  9. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...bar is set very high from last year’s impressive entries, but we have faith our community of creative scientists...

  10. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As we’ve shared these...

  11. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...

  12. We’re Going Viral This Week!

    Type
    Blog Post
    ...platform for scientists using AAV to find and contribute experimental information, such as titer, serotype...

  14. The Twelve Days of CRISPR

    Type
    Blog Post
    ...third day of CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the ...

  22. Countdown to Halloween @Addgene

    Type
    Blog Post
    ... it. Can you guess each plasmid? Our lab paid tribute to the changing seasons – and they also invited...

  27. Editor's Choice, July 2016

    Type
    Blog Post
    ... To better highlight the great content contributed by our bloggers each and every month, we've decided...

  30. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... labs save time and resources by storing and distributing their plasmids with the scientific community...

  31. A Scientist's Guide to Ebola

    Type
    Blog Post
    ... most recent cases, primarily in West African countries. Even that grim estimate might prove to be an ...

  33. Addgene's Website Update

    Type
    Blog Post
    ...depositing data, instead of materials,  there’s a “Contribute to the Data Hub” link in the Deposit bar. Note...

  42. Let There Be LITE Plasmids

    Type
    Blog Post
    ...can be applied non-invasively, its use is not restricted to cultured cells but extends to freely moving...

  50. Science as Art

    Type
    Blog Post
    ...competition that last year received over 2,000 entries. Do you mix your passion for art and science? Do...
Showing: 601 - 650 of 884 results