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Showing: 601 - 650 of 964 results

  1. Deck the Lab 2022!

    Type
    Blog Post
    ...reveal the winning entries on Twitter and Instagram! Happy decorating, all, and to all a good light!    🤩 ...set a challenge to my lab mates. They did not disappoint. #deckthelab pic.twitter.com/X4eKUGnQMe— Alex...

  2. Call for Guest Bloggers

    Type
    Blog Post
    ... Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. Post: Generating...Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein College of...

  5. Summer Fun at Addgene!

    Type
    Blog Post
    ... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

  6. CRISPR Cheat Sheet

    Type
    Blog Post
    ...an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...

  7. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...working in the lab. Do not eat, drink, chew gum, or apply makeup in the laboratory. Before you start your ...and uncluttered. Disinfect your workspace with appropriate materials. For example, you may use 70% ethanol...your lab supervisor what is available and the appropriate contact times for each agent. Make sure you have...autoclaved. Conclusion Although simple, following appropriate BSL-1 and BSL-2 protocol goes a long way. It ...

  8. AAV Packaged on Request is Here!

    Type
    Blog Post
    ... in 100 µL aliquots, so the volume options will appear as 10, 25, or 50 units.  Once your request is received... you find this service useful in your research. Happy vectoring! ...

  9. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ... certain mystique that makes them difficult to approach for the youngster just starting in the lab, but... a graduate student? 4:50 - 8:00 : How did you approach doing rotations? 8:01 - 11:25 : How did you decide...

  12. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...when budgets are constrained. This collaborative approach eliminates redundant work, saving time and allowing...be listed as direct costs from a CRO in grant applications, addressing the new funding structures and indirect...

  14. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ... shipped! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years! We're ... as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently...

  15. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...talks, symposiums, poster sessions, exhibit booths, happy hour socials, and late, late night socials. As a...depositing scientists at #SfN14. And we've been so happy to hear that we've been able to help their labs ...

  16. Addgene's Website Update

    Type
    Blog Post
    ...: The Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as ...through the yellow popup at the bottom of the page. Happy surfing!   This post was written by Addgenies Jo...

  22. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...

  24. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...emphasizes that people should “follow their passions, be happy, and match their personal goals to their career ...analytical and critical thinking skills that are applicable to a variety of careers outside of academia. ...

  25. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...expression observed using transient transfection approaches, generating cell lines using lentiviral vectors... tubes, VWR 21008-216 Lentivirus preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you.... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. This is the beginning of the selection...

  26. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...individualized optimizations, safety testing, and approval. In contrast, a system that can deliver a healthy...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...

  27. Fostering communication in remote collaborations

    Type
    Blog Post
    ...willingness of the team to adopt various tools, this may happen over email or using rapid communication tools such...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the...

  28. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However...antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions prevents FOP bone...

  29. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ... at least we can reduce the rate at which that happens and reduce the manufacturing of new containers,...bin or box in your tissue culture room for the wrappers and ship them back. Start a glove recycling ...

  31. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...managers and some take on leadership roles. You may be happy with a primary role of doer for your entire career...they miss the hands-on bench work. To really be happy, you may need to find a way to do, manage and lead...

  32. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...respond to the employee’s level of discomfort appropriately to get the message across. Listen to our podcast...to you. What are We Learning: What would have happened if _________? What would have been a better outcome...

  34. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline...

  35. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...bottom. Add 5 mL of Pierce IgG Elution Buffer to the capped Gravitrap column and incubate for 10 min at RT...subsequent centrifugation step Pro-Tip Resin will appear compacted after centrifugation. Discard the flow...column in a new 50 mL LoBind collection tube. Slowly apply the sample to the center of the compact resin bed...exchange and concentration with an Amicon column Apply 15 mL of PBS to the reservoir of a Amicon Ultra-...

  40. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...References Biddy, Brent A., et al. "Single-cell mapping of lineage and identity in direct reprogramming...

  41. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...

  43. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis...

  44. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools...

  45. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ... work.  And while these pieces aren't always appropriate for your formal scientific communications, they...still get invested in the journey? The same thing applies for science storytelling. You can introduce your...

  46. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...categories: ones induced by the researcher and ones that happen naturally.  Sample Denaturing  First, let’s think...SDS-page gel. As the protein unfolds, new epitopes may appear, while others may get hidden. Monoclonal antibodies...

  50. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...
Showing: 601 - 650 of 964 results