...it’s true that adding histones and chromatin architecture to every diagram portraying some aspect of eukaryotic...when I recently attended the 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium...but also specifically study it. They might be researching enhancer elements that control the transcription... or indirectly--many other areas of biological research. If you’re looking for resources that will help...posters I saw at the 2018 Keystone Chromosome Architecture and Chromosome Organization. These aren’t the...Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru ...’re looking for on this list, please consider searching directly on our website. In-exhaustive Chromatin-related...

...)” — Jim Woodgett, biomedical researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene...for the research community once more. Our ready-to-use viral vectors were useful for researchers wanting...pcDNA-p160MBP, while the first request for a kit came on March 9, 2005. It took almost six years to reach our first...10 year anniversary.   Expanding our work In March 2015, Addgene reached the 500,000 plasmids distributed...launched the Blue Flame Award, which recognizes researchers who have at least one plasmid deposited at Addgene...-19 pandemic, and Addgene pivoted to support researchers combatting the virus. Our COVID-19 collection... comprised of 1302 plasmids, was shared with researchers across the globe. As the pandemic slowed, Addgene...

...fluorescent proteins in their research. These awards help early career researchers attend a conference of their...opened up applications to research technicians and undergraduate researchers, in addition to postdocs ...postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where.... Below, read about the five winners and their research.   Abhi Aggarwal: Glutamate sensing reporters... Pedre Pérez is a postdoc at the German Cancer Research Center (DKFZ) who has developed new redox sensitive...that detect new redox metabolites are vital for researchers to better understand the processes that happen...University in the department of Physiology. Her research focuses on the neural circuitry involved in mechanical...

...consider while writing each section. Research challenge The Research Challenge motivates the request for...lead to new milestones in the field. Research strategy The Research Strategy highlights i) the significance...Research at universities typically requires funding from a variety of government and academic institutions...overview information, key dates, funding description, research plan, award information, eligibility information...aims requested in the call. Step 2: Develop a research plan Most grant applications restrict the space... and ii) how you, the expert, will address the research challenge. Now is the time to make bold statements...that your solution is the best approach to the research challenge. Demonstrating expertise in the field...

...to expect: Search bar The biggest change is to our most frequently used feature: the search bar. We’ve... now has two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is... the search box to go straight to your materials of interest!   Figure 1: The new search bar.   Pro tip...is our same search bar. And if you’re in the mood to browse our catalog, you can use the links under the... tip! You can use a manuscript title to search for plasmids from a specific paper. If you’re ready to ...deposit, simply click on “Deposit Materials” and the search bar will change to an easy-to-use depositing guide...common activities can be done straight from the new search bar, but the menu just above it can help you quickly...

...scientific research would be very competitive, and so I applied for a summer role as a Research Scientist...network around the world. The research project was hard; I was researching the epidemiology of breast cancer...perfect job. Summer work experience in cancer research After graduating with a 2:1 BSc in Molecular Biology...Scientist at the Institute of Cancer Research in Sutton, located on the edge of London to gain relevant experience... experiments like PCR and running gels, but in research you also experience a lot of failure and problem...and problem solving scientists experience during research. Working abroad in rural Africa Once this position...dissertation. I chose to carry out my dissertation research in a lab in California. I felt then, and still...

... by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high...high-throughput profiling New England Biolabs® (NEB) researchers recently published a method for the high-throughput...blogger, Greg Lohman! Greg Lohman is a biochemistry researcher at New England Biolabs, specializing in the enzymology...Thermus thermophilus DNA ligase." Nucleic acids research 24.15 (1996): 3079-3085. PubMed PMID: 8760897....in duplex DNA by T4 DNA ligase." Nucleic acids research 10.5 (1982): 1425-1437. PubMed PMID: 7041091. ...can be sealed by T4 DNA ligase." Nucleic acids research 15.21 (1987): 8755-8771. PubMed PMID: 3684572....mononucleotide repeat sequences." Nucleic acids research 27.24 (1999): e40-e47. PubMed PMID: 10572192. ...

...idea of the themes in the field, I searched for recent research papers on these particular themes, for... your ability to write, to read efficiently, to search the literature, and to synthesize a large volume...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review... the field you plan to review. If it’s a well-researched field, you may need to get specific to make sure... has the benefit of exposing you to relevant researchers in the field. Finally, you can sound credible...an expert in the field. To find reviews, I just searched online and found ones that I thought “looked good...cared about. I also used reviews to get a list of research papers that I needed to read. Once I had an idea...

...distribution of plasmids for COVID-19 research is accelerating essential research in laboratories all around the...sometimes wonder if we can take another blow. Back in March, we never expected to be in this here and now. Our...Here's the message I shared with the Addgenies on March 11, 2020 after rumors spread in our building that...scientists around the world mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we...proud of the work we’ve done to support COVID-19 research. Our collection of available materials is growing...the crucial role Addgene plays in accelerating research. Yet this was still not enough to ensure we could... doing almost every type of biomedical science research. Our work accelerates and enhances productive ...

...and an inability to improve upon these critical research tools. Addgene is excited to announce that we ...problem.The grant, which is part of the NIH’s Brain Research through Advancing Innovative Neurotechnologies...high-quality antibodies/nanobodies for neuroscience research and is an expert at validating these tools. While...offer numerous advantages, including more secure archiving, unambiguous molecular definition, and potential...the most commonly used reagents in neuroscience research today, but there is a critical unmet need for ...financially self-sustaining through distributions of research materials, such as plasmids and viral vectors....acquisition of affinity reagents and will enable researchers everywhere.” The timing is ideal as the NIH’...

...individuals whose research ranged from wildlife conservation to abortion law to poetry. A research discipline...not ultimately translate to a desire to do the research itself. Several years of exclusively writing lab...depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps; and an essay on the life...versatility in the face of disparate spheres of research. In my case, an interest in neuroscience was carried...occupied my attention, until these coalesced into a research topic on concussion in sport and the public’s ...sport my entire life, I was curious as to what the research had to say. Moreover, this subject allowed me ...public utilizes insights gleaned from scientific research is, after all, one of the enduring goals of science...

... Overview of the CRISPR classification hierarchy. The hierarchy flows from Class to type to subtype to...a decade of frenzied interest in bacterial and archaeal immune systems. During this time, scientists discovered...perhaps provide insights and ideas for your own research! Classification Structure Principles of CRISPR...fast evolution of these adaptive bacterial and archaeal immune systems has led to extreme sequence divergence...classifications were placed into a fairly simple hierarchy: class to type to subtype to variant. Despite ...seemingly complicated steps to arrive at this hierarchy, the main aspects of each class and type are easy...identified CRISPR-Cas in bacteria and nearly 100% in archaea, though Class 1 remains largely underutilized compared...

...proteins have enabled scientists to pursue creative research avenues previously unavailable to them. With these...interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent proteins...Roger Tsien played enormous roles in enabling researchers to utilize and develop these tools by making ...fluorescent protein technologies widely available to the research community. To honor their legacy, we recently ...help scientists share their fluorescent protein research with the academic community by funding their travel...demonstrated the utility of these FLiPs in a variety of research applications and particularly notes their ability...inducible enzyme technologies. She hopes that other researchers will be able to use similar designs to create...

...publication.   Do research! Nearly everything we write entails a primary literature search to collect up ...webinars, or use other research methods. In the case of the antibody guide, primary research and previously ...easily and quickly access tools to further their research. However, tools aren’t very useful if you don...goal is to provide informative content to help researchers in creating and sharing knowledge, related to... types of materials we help distribute and the research communities that we serve. How we curate (and...you didn’t know you wanted to know about, like research breakthroughs or cool new tools. And while this... Center, help.addgene.org, where you can easily search articles answering all of our frequently asked ...

... the progress of research, they can also present hurdles for the team of researchers involved. Communication... where researchers spent time in each other’s labs. This is an opportunity for the researchers involved...Communication is a major challenge when researchers are communicating across disciplines or doing different...or Google Hangouts is a great way to include researchers from different locations. Having virtual face-to-face...best for them. This also accelerates the pace of research by circumventing the slower, sequential communication...document. Set and manage expectations When the researchers involved in a project are scattered across the...future directions of the project. The state of research is constantly shifting, and long-distance collaborations...

...describe all cell lines used in research, using four methods to search out cell lines: Periodic surveying...approximately one-third of cell lines used in scientific research are misidentified! Rigor and reproducibility of...collections websites and catalogues Literature searches utilizing Google Scholar alerts and the NCBI LitSuggest..., such as their vaccine production, SARS-CoV-2 research, or adenovirus packaging cell lines dataset collections...was described. Using Cellosaurus Cellosaurus is searchable by cell line name (recommended names and synonyms...You can also use the Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your...adjusted based on query, reference, and shared allele search preferences to help you identify proximal lines...

...pAAV.hSyn-FLEX.iGABASnFR.F102G pAAV-Syn-Archon1-KGC-GFP-ER2 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] pAAV-FLEX-tdTomato...constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) ...iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical ...brain was measured with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal...imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction with optogenetic...this membrane-embedding voltage detector. Find Archon1 and its cre-dependent version at Addgene! Fluorescent... viral protocols and videos Find AAVs for your research ...

...Having seen CRISPR’s success in basic research, researchers are eager to apply it in a clinical setting...Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research ...preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections Pages Duchenne muscular... around 30 years of age. Despite the amount of research conducted on DMD, there is still no good treatment...it difficult to deliver via gene therapy, so researchers have set their sights on another approach. Since...oligonucleotide treatment comes with many challenges, researchers have begun to explore genome editing approaches...CRISPR-mediated exon skipping approach in mice, DMD researchers are very excited. This approach may also be applicable...

...skills required Research skills The ability to think critically and carry out in-depth research is applicable...career, do your research Once you have identified possible careers, the next step is to research what each ...Epidemiologist Scientific Recruitment Meteorologist Research and Development Healthcare Consulting Lab Manager...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy...include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor Lab Manager...Before you begin to apply for a job, it is useful to search online job websites to see what requirements or...Once you’ve assessed your skill set and done your research, it’s time to start sending out applications. ...

... CRISPR technologies available to the academic research community. At Addgene, we’ve helped distribute...provide ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide CRISPR as virus...proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute for Biologically Inspired...can make lentivirus production frustrating - a researcher can complete an entire lentiviral production ...receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, use next-generation...reagents from Addgene can greatly expedite a researcher's ability to make discoveries." If you ever run...plasmids to create your own lentivirus stocks. Our research scientists have provided their standard lentivirus...