Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 960 results for: Cre

Showing: 241 - 260 of 960 results

  5. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...

  6. Chemogenetics Plasmids

    Type
    Collection
    ...PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...

  8. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast codon-optimized...

  9. Resolute Plasmid Collection

    Type
    Collection
    ...pooled libraries are useful tools for genetic screening experiments. These libraries target the solute...

  12. Synthetic Biology - Overview

    Type
    Collection
    ...Standard Biological Parts - Repository of community-created BioBricks JBEI - Joint Bio-Energy Institute Synberc...

  14. Promega Plasmid Collection

    Type
    Collection
    ...editing outcomes, and conducting high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link...

  15. p53 Pathway

    Type
    Collection
    ...disrupt its interaction with negative regulators, increase its stability and DNA binding activity, and allow...

  16. Open Enzyme Collection

    Type
    Collection
    ...pOpen-PstIR PstI 165528 pOpen-SfiIR (cDNA) SfiI (used to create cDNA libraries) 165529 pOpen-DpnIR DpnI 165537 ...

  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ..., HEK293, COS, CHO), from which the R-mAbs are secreted into the culture media. For high level expression... Gavira-O'Neill CE, et al. 2020. Development, Screening, and Validation of Camelid-Derived Nanobodies ...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...loxP-stop-loxP cassette into the Rosa26 locus, allowing for Cre-dependent expression of a GMAP inserted gene. The...of a good screening experiment can be found on our blog post entitled “Genome-wide Screening Using CRISPR...Receptor-ome Expression and Screening via Transcriptional Output-TANGO (PRESTO-TANGO) to screen the NCC-1 library...and later create stable cell lines expressing it. The Church lab fixed this issue by creating a new dCas9...  CRISPR pooled libraries enable researchers to screen the entire genome for genes that regulate a wide...CRISPR knockout library and used this library to screen for genes involved in West Nile Virus (WNV) induced... gRNAs that confer protection to WNV. From this screen the authors were able to identify 7 previously ...

  20. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...Wet transfer Increase transfer time Increase voltage Increase SDS/decrease alcohol in transfer...Semi-dry transfer Decrease voltage Decrease transfer time Decrease SDS/increase alcohol in transfer...over-transferring, increase the amount of alcohol and decrease the amount of SDS, with a decreased transfer time...under-transferring, try increasing the amount of SDS while decreasing the amount of alcohol. The increased SDS will ... density of the bands, which increase as protein concentration increases, show the protein concentrations..., you’ll see the signal increase as the concentration of the protein increases (Figure 3A).  This step...linear range (i.e., expression decreases as protein concentration decreases).     Figure 3: Testing...
Showing: 241 - 260 of 960 results