Skip to main content

We narrowed to 655 results for: EPO

Showing: 241 - 260 of 655 results
  1. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...and were suggested to be the best gRNAs by the depositing lab. The different labs use different algorithms... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ...
  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... tRNA suppression plasmids are available from depositing labs that have mastered this technique so it ...measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you can insert...protein kinase dynamics in living cells with FRET reporters." Methods 40.3 (2006): 279-286. PubMed PMID: 16908183...
  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ... is not efficient at the protein level at the timepoint studied Off-target activities of the RNAi Correctly... need to study protein expression at multiple timepoints. When the target of interest is critical to cell... specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019...
  4. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution... iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28...
  5. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that have been shared...
  6. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas...help increase global access to materials in our repository. This incredible gift will allow more people ...
  7. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab.   ...
  8. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...kb DNA molecular weight ladders were recently deposited with Addgene. A team of undergraduate students...Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598-017-02693-1. PubMed PMID...
  9. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...
  10. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...Milling and Markson et al., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in ...system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that...
  11. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...
  12. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...
  13. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take...
  14. Luciferase Plasmid Collection

    Type
    Collection
    ... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...for gene expression assays and bioluminescent reporters. Plasmid...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...-lives, making them useful as transcriptional reporters, but are less-than-ideal protein tags due to their...or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids are meant...luciferase and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions...
  15. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...
  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid...Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 ...Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO...EcAR7 Plasmid 52054 SepOTSµ ( aka. B40 OTS) Strain 34929 BL21ΔserB Plasmid 34624 SepOTSα tRNA-Sep ( aka. ... Depositor Collections Rinehart Lab Phosphoprotein Reagents...#188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important technical note:...optimized phosphoserine orthogonal translation system, SepOTSλ (Plasmid #68292) , they were able to demonstrate...
  17. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place... complexes. John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification...
  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Neurotransmitters Cell Physiology Browse All Other Reporters Additional Resources Fluorescent biosensors are...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...Calcium Ratiometric calcium biosensors from nested reporters of green- and orange-emitting proteins Ratiometric...
Showing: 241 - 260 of 655 results