We narrowed to 639 results for: ESP

1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   Blog Post

   ...no or minimal reduction in emission readout, respectively. mNeonGreen can be used as a fusion tag for ... maxima were red-shifted (462 nm and 492 nm, respectively), they gave this a teal classification rather...

2. Plasmids 101: Origin of Replication

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   ... is positively regulated by RNA or proteins, respectively. A plasmid's copy number has to do with the ... contribute as well. These considerations are especially useful to keep in mind if you are planning to...

3. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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   ...Since the start of this crisis, we’ve been very responsive to the mood of the Addgenies — and they were ...said CZI Head of Science, Cori Bargmann. "CZI is especially proud to support Addgene now, when their distribution...

4. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ...Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular calcium levels within ..., which in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because...

5. Meet the New Addgene Board Members!

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   ...different aspects of manufacturing and quality, especially with regard to compliance with health authority...cancer cells under drug treatment.” Stegmeier especially finds the value in the wealth of resources on...

6. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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   ...more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial cultures....org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito D (2007) Improved recombinational stability of...

7. Multifunctional Neural Fibers: Modulation from Gut to Brain

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   ...outcomes of interest, and stimulate or manipulate responses through neurological means like electric stimulation... They observed increased vagus nerve firing in response to both of these stimulations. The best part? ...

8. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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   ...replacing its dimerization domain with VVD, a light-responsive domain that dimerizes upon exposure to blue light... Ventura B (2021) Engineering AraC to make it responsive to light instead of arabinose. Nat Chem Biol....

9. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   ...untreated and sucrose treated viral preparations.    Despite observing a positive effect on our overall yield...Although we were unable to deduce the mechanism responsible we still believe that these results will be beneficial...

10. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    ... time, the use of episome seemed appropriate, especially since the Fertility, or F-factor discovered by...scientific community. Prior to this, bacteriophage, especially lambda, was the tool of choice for molecular ...

11. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ...explore the functional differences among mutations responsible for the disease known as Alternating Hemiplegia...disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3. The gene encodes a...

12. Creating accessible biology activities in schools with BioBits

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    ...equivalent of a “chemistry kit” for topics in biology, especially those at the molecular and cellular level. The... of this is conducive to a classroom setting, especially for a school with a limited budget. While there...

13. What Good Citizenship Can Do for Reproducibility in Science

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    ... can and do play this role, and we take our responsibility to push for clear communication of science ...broader role as Vice President. Her current responsibilities encompass overall leadership of the Cell Press...

14. New and Upcoming Viral Vectors - September 2019

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    ...specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action potential...brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest resting...

15. Reagent Repositories Are Speeding up Science During the Pandemic

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    ...they provide rapid access to validated reagents, especially during a time when these materials may not be...of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https:...

16. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    ...methods are more sensitive than UV absorbance, especially when you expect low concentrations in your samples...samples with a known DNA quantity and their corresponding fluorescence. That way, you can compare the ...

17. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    ...using CRISPR/Cas9. They are targeting the genes responsible for both early onset and late onset AD. In this...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

18. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    ...by glutamate, and tweaking it to improve its responsiveness, they showed that iGluSnFR could detect neuronal...sensitive and better localized for synaptic glutamate response than its predecessors. The detailed characterization...

19. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    ...classify unknown, or ‘orphan’, TFs into their respective families. Given the number of poorly studied ...cellular fates were then matched with candidate TFs responsible for that differentiation.   Fig. 2: Single...

20. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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    ... illustrates the power of collaboration, which despite the competitiveness of cutting-edge research, is... list. We then collected and classified their responses, and used that information to deliver to the requesters...