We narrowed to 636 results for: ESP

1. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

   Type

   Blog Post

   ...untreated and sucrose treated viral preparations.    Despite observing a positive effect on our overall yield...Although we were unable to deduce the mechanism responsible we still believe that these results will be beneficial...

2. Plasmids 101: Origin of Replication

   Type

   Blog Post

   ... is positively regulated by RNA or proteins, respectively. A plasmid's copy number has to do with the ... contribute as well. These considerations are especially useful to keep in mind if you are planning to...

3. Safe Port in a Storm...How Addgene is Weathering the Pandemic

   Type

   Blog Post

   ...Since the start of this crisis, we’ve been very responsive to the mood of the Addgenies — and they were ...said CZI Head of Science, Cori Bargmann. "CZI is especially proud to support Addgene now, when their distribution...

4. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   ...Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular calcium levels within ..., which in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because...

5. Meet the New Addgene Board Members!

   Type

   Blog Post

   ...different aspects of manufacturing and quality, especially with regard to compliance with health authority...cancer cells under drug treatment.” Stegmeier especially finds the value in the wealth of resources on...

6. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

   Type

   Blog Post

   ...more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial cultures....org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito D (2007) Improved recombinational stability of...

7. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   ...replacing its dimerization domain with VVD, a light-responsive domain that dimerizes upon exposure to blue light... Ventura B (2021) Engineering AraC to make it responsive to light instead of arabinose. Nat Chem Biol....

8. Multifunctional Neural Fibers: Modulation from Gut to Brain

   Type

   Blog Post

   ...outcomes of interest, and stimulate or manipulate responses through neurological means like electric stimulation... They observed increased vagus nerve firing in response to both of these stimulations. The best part? ...

9. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

   Type

   Blog Post

   ... time, the use of episome seemed appropriate, especially since the Fertility, or F-factor discovered by...scientific community. Prior to this, bacteriophage, especially lambda, was the tool of choice for molecular ...

10. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type

    Blog Post

    ...explore the functional differences among mutations responsible for the disease known as Alternating Hemiplegia...disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3. The gene encodes a...

11. Creating accessible biology activities in schools with BioBits

    Type

    Blog Post

    ...equivalent of a “chemistry kit” for topics in biology, especially those at the molecular and cellular level. The... of this is conducive to a classroom setting, especially for a school with a limited budget. While there...

12. Components of CRISPR/Cas9

    Type

    Blog Post

    ...is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...

13. What Good Citizenship Can Do for Reproducibility in Science

    Type

    Blog Post

    ... can and do play this role, and we take our responsibility to push for clear communication of science ...broader role as Vice President. Her current responsibilities encompass overall leadership of the Cell Press...

14. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    ...specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action potential...brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest resting...

15. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type

    Blog Post

    ...that can sense contaminants and emit light as a response. To create this bacterial sensor, he will construct... activate fluorescent protein transcription in response to river contaminants and transform these plasmids...

16. Reagent Repositories Are Speeding up Science During the Pandemic

    Type

    Blog Post

    ...they provide rapid access to validated reagents, especially during a time when these materials may not be...of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https:...

17. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type

    Blog Post

    ...methods are more sensitive than UV absorbance, especially when you expect low concentrations in your samples...samples with a known DNA quantity and their corresponding fluorescence. That way, you can compare the ...

18. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type

    Blog Post

    ...classify unknown, or ‘orphan’, TFs into their respective families. Given the number of poorly studied ...cellular fates were then matched with candidate TFs responsible for that differentiation.   Fig. 2: Single...

19. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type

    Blog Post

    ...using CRISPR/Cas9. They are targeting the genes responsible for both early onset and late onset AD. In this...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

20. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type

    Blog Post

    ...by glutamate, and tweaking it to improve its responsiveness, they showed that iGluSnFR could detect neuronal...sensitive and better localized for synaptic glutamate response than its predecessors. The detailed characterization...