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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 671 results for: FIE

Showing: 241 - 260 of 671 results

  1. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...gRNA is defined by the user, and it can be easily modified to target Cas9 to virtually any genomic locus,.... Figure 2: The CRISPR library must be amplified (A) to be used to generate lentivirus (B). Cas9... Mutant cells are screened (D) and "hits" are identified using next-generation sequencing (E). Considerations...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ..., you can learn more about how nanobodies are identified in this review. If you just want to use the nanobodies...cleaved by the bdNEDP1 protease, thus releasing purified nanobodies from the column. bdNEDP1 protease can... using plasmid pDG02583. Label the Nanobodies: Purified nanobodies are then tagged with a fluorescent ...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...that your antibody is produced, harvested, purified, quantified, and validated, it’s ready for use! You ...PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...relationship between the genetic dependencies they identified and their associated ancestries. While there ...there were some exciting relationships identified, 48% were found to be artificial, so that didn't seem a ... they found that for approximately 40% of the identified genes, the genes were not expressed at all. This...

  5. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...lab expertise, experience in different scientific fields, and knowledge of how to troubleshoot problems ...product under conditions different from those specified on its data sheet?” or “Do you have additional...excited to hear about novel developments in the fields of gene and cellular therapies.     Additional ...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...our primary target audience – colleagues in our field. Secondary audiences that may also benefit, such...quickly synthesize information from a variety of fields in order to provide useful solutions to complex... of 865,995 views for the 926 video abstracts identified. The journal Cell had the largest average view...

  7. The Challenges of Cell Culture

    Type
    Blog Post
    ... Cell culture is a widespread tool used in the fields of oncology, virology, immunology, microbiology...this reason. Unreproducible results are rife. Misidentified or cross-contaminated cells have been known ... database listing all cross contaminated or misidentified cell lines. The solution Fortunately it has ...

  8. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...scientists monitor an animal’s movement in an open field box or a circular corridor apparatus using a camera...fear. Such a behavior can be monitored in an open field test (Alpar et al., 2018). Locomotion can also be...Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that activates neurons upon light...

  9. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...celebrated by the Nobel prize committee). But as the field of genetics progresses and genome sequences become...gene deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected... in developmental biology, and advances in this field and others are likely to continue with help from...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    ...script block of the Nextflow process.  Next, we specified the input and output channels for the initial ...basis, but for rapid testing and refinements, we specified a select small number of input file names or filtered...created trimmed FASTQ files as output, which we specified as input to the subsequent bbmerge process.   ...

  11. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Addgene distributes to per year, 2005-2023. We identified and offered a number of solutions that increased...consistently accessible, wasn’t an option. So we identified countries that would benefit from a third-party...On individual material pages, we added curation fields for purpose, promoters, expression types, and more...

  12. Hot Plasmids - November 2023

    Type
    Blog Post
    ...dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels (which had therefore...locations. C) Enrichment of dual-labeled proteins identifies proteins that had been trafficked from the “source...collection since we began distribution and has been verified to perform well in standard IHC and western blot...

  13. CRISPR Library Amplification

    Type
    Protocol
    ...transformation and basic knowledge of the library being amplified. Please see our protocol on Bacterial Transformation... change in representation compared to the pre-amplified stock. A last option is to perform a gel extraction...extraction of either the original sample or the amplified sample, followed by reamplification of the DNA,...

  14. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... selection, individual gene edited clones are identified by PCR. Then, if necessary for subsequent experiments...described in several recent reviews (Howes and Schofield, 2015; Moser and Hirsch, 2016; Epstein and Schaffer...PMCID: PMC5568673. 8. Howes, Rob, and Christine Schofield. "Genome engineering using adeno-associated virus...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...saturation mutagenesis. Error-prone PCR: This method amplifies the wild-type template with a “sloppy” version... each template. The PCR product is then column purified. Degrade wild-type template strand: Next, the ...Fowler, Douglas M., Jason J. Stephany, and Stanley Fields. “Measuring the Activity of Protein Variants on...

  16. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...graph representing all insertions and deletions identified within a given genomic window, along with the...on a gel to look for size shifts. Once you’ve identified homozygous clones, however, sequence confirmation...knock-in edit small, with only a few base pairs modified? In these cases, it’s best to do some planning...

  17. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... end_module_attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...research arsenal, and there is still a dedicated field of study for improving these tools. Next time you...

  18. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...Assumption #2: The loading control can be accurately quantified In western blotting, the relationship between...Assumption #2: The loading control can be accurately quantified Most housekeeping proteins are highly expressed...Assumption #2: The loading control can be accurately quantified Because the total protein is measured in a separate...

  20. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Cas9 is revolutionizing the mouse gene-targeting field. Mice have long been extremely useful in the lab...components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease that creates...Z, Huang J (2016) Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9. Sci Rep...
Showing: 241 - 260 of 671 results