We narrowed to 342 results for: GAI

1. Antibodies 101: Stripping and Reprobing Western Blots

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   Blog Post

   ...from your membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample...

2. Hot Plasmids February 2024

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   Blog Post

   ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

3. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   Blog Post

   ...departments across the university. Collective bargaining may provide a better deal for students in areas...

4. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR in a “second chance” mechanism...

5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...desribed how P. furiosus has systems to defend against both DNA and RNA viruses - 2 cas systems regulate...

6. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ...fluorescent units and so lowers the FRET signal back again. The Ting lab constructed 2 versions of this reporter...

7. The Materials Science of Optogenetics Experiments

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   Blog Post

   ...opportunity to “reset” and become receptive to light once again. Therefore, light must be delivered in pulses. An...

8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...were capable of cleavage, they tested the clones against an NNN PAM library in bacteria where cleavage leads...

9. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database, and it...

10. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

    ...We test the (capped) 1:1 agarose/DMEM solution against a part of the body very sensitive to heat- the ...

11. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...However, much knowledge about functionality can be gained by understanding the location and transport of ...

12. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post

    ...recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients with an integrated...

13. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post

    ...University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg ...

14. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional Resources on...

15. PCR Applications for Copy Number Variation Assays

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    ...price point, which should be carefully balanced against the limitations of the assay.      Fig....

16. Transferable Skills: Negotiation

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    Blog Post

    ... company. How can I negotiate with my manager to gain experience in the area I want to explore while also...

17. Hot Plasmids: Winter 2025

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    Blog Post

    ...Ashley Waldron We recently added two antibodies against important targets for neurodegeneration research...

18. Magnetic Control of Proteins: More than a Dream

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    Blog Post

    ...screens of AsLOV2 variants showed some significant gains! After a few more rounds of mutagenesis, they eventually...

19. Of Myc and Men

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    Blog Post

    ...over the years, several alternative antibodies against the Myc tag have been generated in an attempt to...

20. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ... right places. Checks and balances: protecting against cancerous cells Luckily, there are several safety...