We narrowed to 298 results for: IRES

1. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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   Blog Post

   ...different reporter proteins. Protein translation requires the start codon of AUG immediately downstream ...

2. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ... following injection into the bloodstream, but requires a high vector dose and is unable to reach all ...

3. New Tools Enable CRISPRa for Neuroscience Applications

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   ...., 2018). The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to ...

4. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   ...is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate protocols, ...

5. Analyzing CRISPR Editing Results with ICE from Synthego

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   ...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...

6. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   ...passages. Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing...

7. How to Write a Scientific Review Article

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   Blog Post

   ...convert the citation style to whatever the journal requires. Using the (Last name, Year) format also has the...

8. Plasmids 101: Gateway Cloning

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   ...and destination vector creates two products: the desires expression clone and a byproduct containing the...

9. Antibiotic Resistance: An Old Solution but a New Problem

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   ...Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results. While ...

10. Plasmids 101: Broad Host Range Plasmids

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    ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

11. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...Samaco RC, Zoghbi HY (2011) Adult Neural Function Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126...

12. Antibodies 101: Introduction to Immunofluorescence

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    ...visualize their target. In contrast, indirect IF requires two antibodies: a primary and secondary. For indirect...

13. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post

    ...for the scFv (Gaciarz and Ruddock., 2017), but requires an oxidizing environment to form. When expressed...

14. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post

    ...housekeeping proteins.” Fully quantifying a western blot requires a standard curve with a pure sample of your protein...

15. Antibodies 101: Beyond Surface Labeling

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    ... hence, intracellular labeling with antibodies requires chemically pre-treating the cells to allow for...

16. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...sequence preferences at the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target ...

17. Tips for Getting a Faculty Position

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    Blog Post

    ...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to...

18. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post

    ...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...

19. March for Science

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    Blog Post

    ...science rallies scientists to political action, and inspires politicians and citizens to include science in...

20. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...phage-derived uracil labeled template, this method requires further DNA shuffling to combine pairs of mutations...