We narrowed to 298 results for: IRES

...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...

...different reporter proteins. Protein translation requires the start codon of AUG immediately downstream ...

...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...

...passages. Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing...

... following injection into the bloodstream, but requires a high vector dose and is unable to reach all ...

...., 2018). The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to ...

...is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate protocols, ...

...Samaco RC, Zoghbi HY (2011) Adult Neural Function Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126...

...initiation. In mammalian cells, robust repression requires targeting dCas9 along with transcriptional repressors...

...Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results. While ...

...of S. pyogenes Cas9. While the wild-type enzyme requires a PAM sequence of NGG (where N represents any ...

...housekeeping proteins.” Fully quantifying a western blot requires a standard curve with a pure sample of your protein...

... hence, intracellular labeling with antibodies requires chemically pre-treating the cells to allow for...

...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to...

...science rallies scientists to political action, and inspires politicians and citizens to include science in...

...phage-derived uracil labeled template, this method requires further DNA shuffling to combine pairs of mutations...

...visualize their target. In contrast, indirect IF requires two antibodies: a primary and secondary. For indirect...

...for the scFv (Gaciarz and Ruddock., 2017), but requires an oxidizing environment to form. When expressed...

...sequence preferences at the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target ...

...switching from paper to an electronic lab notebook requires time and knowledge. In a recent article, we translated...