We narrowed to 298 results for: IRES

1. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   ... following injection into the bloodstream, but requires a high vector dose and is unable to reach all ...

2. New Tools Enable CRISPRa for Neuroscience Applications

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   Blog Post

   ...., 2018). The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to ...

3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   Blog Post

   ...is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate protocols, ...

4. How to Write a Scientific Review Article

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   Blog Post

   ...convert the citation style to whatever the journal requires. Using the (Last name, Year) format also has the...

5. Plasmids 101: Gateway Cloning

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   Blog Post

   ...and destination vector creates two products: the desires expression clone and a byproduct containing the...

6. Antibiotic Resistance: An Old Solution but a New Problem

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   Blog Post

   ...Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results. While ...

7. Analyzing CRISPR Editing Results with ICE from Synthego

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   Blog Post

   ...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...

8. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   Blog Post

   ...passages. Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing...

9. Plasmids 101: Broad Host Range Plasmids

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   Blog Post

   ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ...initiation. In mammalian cells, robust repression requires targeting dCas9 along with transcriptional repressors...

11. Tips for Getting a Faculty Position

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    Blog Post

    ...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to...

12. The Advances Behind The World's First Personalized CRISPR Treatment

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    Blog Post

    ...of S. pyogenes Cas9. While the wild-type enzyme requires a PAM sequence of NGG (where N represents any ...

13. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post

    ...housekeeping proteins.” Fully quantifying a western blot requires a standard curve with a pure sample of your protein...

14. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post

    ...sequence preferences at the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target ...

15. March for Science

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    Blog Post

    ...science rallies scientists to political action, and inspires politicians and citizens to include science in...

16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ...phage-derived uracil labeled template, this method requires further DNA shuffling to combine pairs of mutations...

17. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    Blog Post

    ...switching from paper to an electronic lab notebook requires time and knowledge. In a recent article, we translated...

18. Antibodies 101: Beyond Surface Labeling

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    Blog Post

    ... hence, intracellular labeling with antibodies requires chemically pre-treating the cells to allow for...

19. Hot Biosensors 2022: Year-End Roundup

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    Blog Post

    ...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...

20. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post

    ...visualize their target. In contrast, indirect IF requires two antibodies: a primary and secondary. For indirect...