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We narrowed to 526 results for: MEA;

Showing: 241 - 260 of 526 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose cannot be taken up by cell...common lab strains; however, these tables are by no means exhaustive! For a more comprehensive list and additional...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...target within ~30 nt of your proposed edit, which means there are so few gRNAs to choose from that sequence...of the repair template (Yang et al., 2013). This means that, for gene editing, there are usually few potential...

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...then prevents transcription from occurring, which means that transcription is now OFF.   We’ll look at ... 3 binary systems listed above are orthogonal, meaning that they do not affect each other. For example...

  6. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... western. To prepare the samples for SDS-PAGE, measure the protein content and normalize to ensure equivalent...gauge protein abundance they provide qualitative measurements. If you require precise quantification of a ...

  8. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ... the clinic. At the bench, improving antibodies means improving research. More specific antibodies support...display are common. ELISA enables quantitative measurement of antigen-antibody interactions in a plate-based...

  10. Typing CRISPR Systems

    Type
    Blog Post
    ...mechanisms of action and other analyses. This also means that between two types, the Cas effector may have...nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu, F. E., Moeller, L., Kocalar...

  11. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...microscope before it reaches the detector. This means that two or more objects lying within the diffraction...axial dimension that will appear in focus). This means that the images will appear sharp without interference...

  12. DNA Quantification

    Type
    Protocol
    ...and click measure, be sure to record the concentration and purity. Note: Purity is measured under the ...specific wavelength that they maximally absorb. By measuring the absorbance of a liquid you can accurately ...substance in that liquid. In order to accurately measure the concentration of a substance based on its absorbance...specific to your lab. Spectrophotometer Tips Before measuring any samples, be sure to ‘blank’ the spectrophotometer...resuspended in, but with no DNA added. 'Blanking' measures the background inherent to the machine and your...your solvent. If using a NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal...

  13. Optogenetics Guide

    Type
    Guide
    ...optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons) and their...optogenetics research utilize light to detect, measure, and control molecular signals and cells in order...

  14. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...expressed from its own promoter. But that doesn’t mean you have to clone and transfect multiple promoter-gRNA...gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler sequences...

  15. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... etc. (more on prep sizes below). But what do we mean by DNA quality? There are a few different issues...?  Obtaining higher quantities of DNA generally means starting with higher volumes of bacterial culture...

  17. Adenovirus Guide

    Type
    Guide
    ...and therefore needs to be supplied through other means. In order to produce rAdV vectors, E1 needs to be...Different serotypes exhibit varying tissue tropisms, meaning they preferentially infect certain tissues, which...

  18. Lentiviral Vector Guide

    Type
    Guide
    ...only infect mitotically active cell types. This means that lentiviruses can infect a greater variety of...populations that can be difficult to transduce by other means. A concern with gene therapies is the possibility...

  19. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Biosafety Levels One and Two (BSL-1 and BSL-2) Safety measures for laboratories operating at BSL-1 and BSL-2 ...extract, and precipitate DNA DNA Quantification Measure DNA concentration with a spectrophotometer Restriction...Lentivirus Quantify virus using fluorescence measurements Colony Formation Titering Assay for Lentivirus...by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer of your adeno-associated virus...the Video! AAV Titration by ddPCR Use ddPCR to measure the physical titer of your adeno-associated virus...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...

  20. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAV serotypes exhibit strong neuronal tropism, meaning they can efficiently target specific brain regions...tools developed by researchers are used to detect, measure, and control molecular signals of cells (commonly...
Showing: 241 - 260 of 526 results