We narrowed to 402 results for: MUT

1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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   ...immune measures and correlating them with tumor mutations and clinical responses. This platform has the ...

2. Luminescent Imaging with Nano-lanterns

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   ...described the first Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent...

3. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   ...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...

4. Viral Vectors 101: Viral Applications

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   ... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently...

5. Top Requested Lentivirus and AAV of 2016

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   ...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...

6. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively...

7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ...domain of a Ciona intestinalis voltage sensor and a mutated version of the pHLuorin fluorescent protein. This...

8. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...light-sensitive protein QuasAr2 and systematically mutated it. Using a robotic picking approach to select ...

9. RaPID Detection of RNA-protein Interactions

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   ...early-onset cataracts, is characterized by point mutations in the Iron Response Element (IRE) RNA motif of...

10. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

11. Antibodies 101: Affinity Reagents

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    ...fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used for assembling ...

12. Plasmids 101: The protein expression toolbox

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    ... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...

13. All in a Twist: dsRNA

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    ...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

14. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    ... the protein. To monitor autophagy, the Pho8Δ60 mutant protein is expressed. Pho8Δ60 localizes to the ...

15. New Tool for Lineage Tracing: The ClonTracer Library

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    ...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

16. Plasmids 101: Golden Gate Cloning

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    ...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

17. Plasmids 101: Cre-lox

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    ...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which...

18. Mouse Modeling, Part 1: Genetically Engineered Mice

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    ...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...

19. Overcoming the Challenges of Lentiviral Production

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    ...promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems such that it no longer...

20. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...