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TypeBlog Post...Addgene Cas9 and NgAgo head to head NgAgo has a number of unique features compared to Cas9. These include...
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Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post..., and pUC were developed to provide higher copy number vectors that could be used in these cloning experiments... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...migraines. Those attacks can be brought on by a number of stressors, including emotional stress or changes... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post... response, I was still able to get through to a number of experts, including Harvard/MIT faculty, venture... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...upon the type of transcriptional regulator or the number of designed scaffold binding sites. With hundreds... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...what options lie ahead of me? Like a considerable number of researchers, I had high hopes of securing tenure... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...Leadership Communication Networking As the number of qualified scientists entering the workforce ... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog Post...used as an indicator of DNA purity. Ideally, this number should be between 1.8 and 2.0. The A260/A230 ratio... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...through a phase of crisis where they 'collect' a number of mutations.” Cystic fibrosis may not be the most... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...visualized and results were consistent with copy number variation (CNV) analysis of the U2OS cells. ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...a higher transduction efficiency, i.e. the same number of AAV particles is able to infect more cells (... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...the transcriptional regulation of many genes. A number of groups have designed means of studying the effects... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post..., TFs into their respective families. Given the number of poorly studied TFs, this functional association... -
The MTA at Addgene
TypeBlog Post... process. This is important to keep up with the number of requests and deposits in our repository. From... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...email Addgene at [email protected] with your order number and so it can be prioritized. ... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post...fpvis (complete spectral information, accession numbers, sequences and lineages, FRET efficiencies, additional... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...to be released into suspension and lowering the number of viral particles that can be harvested from the... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...Applications of the Gal4/UAS system There are a number of fancy ways to use the Gal4/UAS system, and you... -
Science Career Options
TypeBlog Post...currently have too many applicants compared to the number of available roles). Make your resume stand out... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...of approximately 1%. This is by no means a large number, but it was attained without any of the error-prone...