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1. Advice for Moving Into Sales After Your Science Postdoc

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   Blog Post

   ...what options lie ahead of me? Like a considerable number of researchers, I had high hopes of securing tenure...

2. Extracurricular Activities for a Strong Science Career Path

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   Blog Post

   ...Leadership Communication Networking As the number of qualified scientists entering the workforce ...

3. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   Blog Post

   ...used as an indicator of DNA purity. Ideally, this number should be between 1.8 and 2.0. The A260/A230 ratio...

4. Scientists Map the SARS-CoV-2-Human Interaction Network

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   Blog Post

   ...email Addgene at [email protected] with your order number and so it can be prioritized. ...

5. FPbase: A new community-editable fluorescent protein database

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   Blog Post

   ...fpvis (complete spectral information, accession numbers, sequences and lineages, FRET efficiencies, additional...

6. The Importance of High Titer for AAV Transductions

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   Blog Post

   ...to be released into suspension and lowering the number of viral particles that can be harvested from the...

7. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   Blog Post

   ...the transcriptional regulation of many genes. A number of groups have designed means of studying the effects...

8. Visualizing Genomic Loci with CRISPR-Sirius

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   Blog Post

   ...visualized and results were consistent with copy number variation (CNV) analysis of the U2OS cells.   ...

9. Improving Transduction Efficiency with Exosome AAVs

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   Blog Post

   ...a higher transduction efficiency, i.e. the same number of AAV particles is able to infect more cells (...

10. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post

    ...through a phase of crisis where they 'collect' a number of mutations.” Cystic fibrosis may not be the most...

11. The MTA at Addgene

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    Blog Post

    ... process. This is important to keep up with the number of requests and deposits in our repository. From...

12. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    Blog Post

    ...Applications of the Gal4/UAS system There are a number of fancy ways to use the Gal4/UAS system, and you...

13. Science Career Options

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    Blog Post

    ...currently have too many applicants compared to the number of available roles). Make your resume stand out...

14. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post

    ..., TFs into their respective families. Given the number of poorly studied TFs, this functional association...

15. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post

    ...of approximately 1%. This is by no means a large number, but it was attained without any of the error-prone...

16. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post

    ...extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent proteins with this...

17. CRISPR Library Amplification

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    Protocol

    ...100 x 100 x 100 ÷ 0.1 This number should be at least 1000X greater than number of perturbations in library...plasmids and can be scaled according to the actual number. Required Quality Control (QC) Pooled libraries...individual plasmids. For larger libraries scale the number of cells and DNA accordingly. Pro-Tip Use of extreme...order to be enumerated accurately. Frequently the number of colonies can be too great to count. Ideally,...a given plate (example: by increasing the total number of bioassay plates used). Scaling up the experiment...Maxiprep protocol, scale the reagent volume and column number as needed. The use of Mega or Gigapreps is acceptable...acceptable when scaling up or reducing the number of tandem purifications. Consult the manufacturers handbooks...

18. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post

    ...biology, cloning a gene into your plasmid is often a numbers game. By using a variety of screening and selection...

19. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post

    ...expressing cells exhibited a 2-4 fold increase in the number of endogenous indel edits when compared to unsorted...

20. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...transcription, or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how...