Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 653 results for: PPL

Showing: 241 - 260 of 653 results

  1. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...the CNS (figure 5 in Chan et al) and the PNS (supplemental figure 8 of Chan et al). These new AAVs can ...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments... transgenic mouse required (see figure 5 and supplemental figure 8 in Chan et al).  References 1. Chan...

  2. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...acetylation) - tri_methylation In the next section we’ll apply the above formula to a real-world experiment. We...therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section. In order to denote...is the foundation of precision medicine. We can apply this knowledge to create targeted disease therapies...

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... use ddPCR for AAV titration, there are many applications for the technology.  Droplet digital PCR is ...being used in the detection of microbes. Some applications include measuring viable probiotics and detecting...detecting circulating pathogens.  With its many applications and ease of use, droplet digital PCR is becoming...

  4. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...

  5. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...multiple harvest strategy is that each day you are supplying your producer cells with fresh medium. Daily medium... freshly collected lentivirus for downstream applications. While this ensures that the highest titer virus...establishes the best conditions for specific applications, stick with them! Changes in such variables ...

  6. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were...place, while increasing the number of potential applications. But they weren’t done engineering quite yet... at the bench and potentially in therapeutic applications. The potential of CRISPR has yet to fully taken...

  7. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...it down until you have something tangible and applicable to your situation.   Thinking Outside the Box... currently plaguing the scientific community is supply chain shortages. When flasks, conical tubes or ...creative skills you have developed in the lab can be applied anywhere! References and Resources References ...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Protein A and Protein G are not suitable for all applications, such as protein isolation from serum. In this...capture antibody interaction and/or the downstream application. Typically, if the target protein will be detected...and an isotype control antibody. Alternative applications Oftentimes, an IP-appropriate antibody simply...

  9. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...property across various software providers used to supplement their paper notebooks. Instead, all of the important...might be available as well. In most cases this applies to universities. How are you currently exchanging... Besides this being of crucial importance when applying for a patent for example, it also comes in handy...

  10. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...that expresses all desired variants of a protein. Applying a selective pressure winnows the pool down to ...mutations are introduced, and scalability (see Supplementary Table 1 of Wrenbeck et al). It uses a uracil...about the same number of positive clones. See Supplementary Note 1 in Wrenbeck et al for details on how ...

  11. Evolution of Lab Techniques

    Type
    Blog Post
    ...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...sequence thousands of reads at the same time. Other applications of sequencing, such as confirming the results...replaced these, these techniques still have useful applications and have informed many current techniques. For...

  12. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...success in basic research, researchers are eager to apply it in a clinical setting. CRISPR is often used for...estimated that exon skipping therapies would be applicable to 80% of DMD patients. Figure 1: Exon...researchers are very excited. This approach may also be applicable to a number of rare genetic diseases caused by...

  13. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... barrier of the endothelium, but they could be applied to study other actin-dependent processes in cells...) and to introduce unnatural head groups for applications like fluorescent labeling via click chemistry...constructs are great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks...

  14. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...Advancing from this invention to KJ’s clinical application in under a decade is an astounding feat of public...of identifying a successful editor. For this application, ABE8e turned out to be most effective. Targeting...editors with increased activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https:...

  15. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...chaperone NPM2, but the technique should be widely applicable to other targets. The modular system of linkers...scFvs for other targets or could be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM...optimizing prime editing systems for specific applications! Delivering prime editors (PEs) via engineered...

  16. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...proxy for dimerization (Shaner et al., 2008 - supplementary figure C). However, these assays are qualitative...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...

  17. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...the coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, ...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

  19. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...missing or added at the broken ends and, as such, cripple the gene. However, if repair material is provided... with Biogen and is particularly interested in applying CRISPR for mouse genome editing.     Haoyi Wang...interested in using CRISPR to develop therapeutic applications that better human health.     References Cong...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...technology that may be used for a variety of research applications, including making transgenic cell lines and ...Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized knowledge to help researchers identify...
Showing: 241 - 260 of 653 results