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We narrowed to 651 results for: PPL

Showing: 241 - 260 of 651 results

  1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ... Tang and Cepko were eager to see if they could apply GFP regulation to other types of proteins. They ...systems are easily adaptable to neuroscience applications, including optogenetics, and they should make...Tang note that their scaffold system is likely applicable to many types of proteins. For proteins with ...

  2. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ... reduced off-target editing frequencies. For applications that require high DNA editing specificity, for...deamination, an important attribute for therapeutic application. ABE8s demonstrate an overall improved base editing...editors with increased activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038...

  3. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods...to increased intracellular calcium. It has been applied to mark active populations of neurons in vivo to...Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction to AAV ...

  4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate ... of chromatin interaction networks  A second application for CAPTURE 2.0 is the construction of spatial...temporal changes in chromatin interactions A third application for CAPTURE 2.0 is detection of temporal changes...

  5. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... experimental, biotechnological, and medical applications. Researches can visualize proteins in vivo by...protein you need   Your plans for downstream applications In this blog post we will summarize some of...acids that are useful in different downstream applications. Cell-free expression systems however, are expensive...

  6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...accuracy of assembly. The fidelity data can be applied to derive similar high-fidelity overhangs for any...Kucera is a Principal Development Scientist of Applications & Product Development focusing on DNA cloning...Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias...

  7. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...

  8. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...not-so-technical skills you’ve built over time are only applicable to those positions you have already held. Imagine...have the desired degree, you have mastered the applications they use, you’ve gained experience working with...identified by another person. Similar reasoning may also apply to other acquired skills.” It is nice when other...

  9. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...the CNS (figure 5 in Chan et al) and the PNS (supplemental figure 8 of Chan et al). These new AAVs can ...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments... transgenic mouse required (see figure 5 and supplemental figure 8 in Chan et al).  References 1. Chan...

  10. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... use ddPCR for AAV titration, there are many applications for the technology.  Droplet digital PCR is ...being used in the detection of microbes. Some applications include measuring viable probiotics and detecting...detecting circulating pathogens.  With its many applications and ease of use, droplet digital PCR is becoming...

  11. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...acetylation) - tri_methylation In the next section we’ll apply the above formula to a real-world experiment. We...therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section. In order to denote...is the foundation of precision medicine. We can apply this knowledge to create targeted disease therapies...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were...place, while increasing the number of potential applications. But they weren’t done engineering quite yet... at the bench and potentially in therapeutic applications. The potential of CRISPR has yet to fully taken...

  13. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...it down until you have something tangible and applicable to your situation.   Thinking Outside the Box... currently plaguing the scientific community is supply chain shortages. When flasks, conical tubes or ...creative skills you have developed in the lab can be applied anywhere! References and Resources References ...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Protein A and Protein G are not suitable for all applications, such as protein isolation from serum. In this...capture antibody interaction and/or the downstream application. Typically, if the target protein will be detected...and an isotype control antibody. Alternative applications Oftentimes, an IP-appropriate antibody simply...

  15. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...multiple harvest strategy is that each day you are supplying your producer cells with fresh medium. Daily medium... freshly collected lentivirus for downstream applications. While this ensures that the highest titer virus...establishes the best conditions for specific applications, stick with them! Changes in such variables ...

  16. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...proxy for dimerization (Shaner et al., 2008 - supplementary figure C). However, these assays are qualitative...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...

  17. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...property across various software providers used to supplement their paper notebooks. Instead, all of the important...might be available as well. In most cases this applies to universities. How are you currently exchanging... Besides this being of crucial importance when applying for a patent for example, it also comes in handy...

  18. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...chaperone NPM2, but the technique should be widely applicable to other targets. The modular system of linkers...scFvs for other targets or could be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM...optimizing prime editing systems for specific applications! Delivering prime editors (PEs) via engineered...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... barrier of the endothelium, but they could be applied to study other actin-dependent processes in cells...) and to introduce unnatural head groups for applications like fluorescent labeling via click chemistry...constructs are great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)...
Showing: 241 - 260 of 651 results