Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 582 results for: RET

Showing: 241 - 260 of 582 results

  1. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...sequencing is successful, your NGS provider should return results in the form of many, many short DNA sequences...specific libraries, but any analysis script should return the same output: a spreadsheet of read counts. ...

  2. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...Peak emission (nm) Size (kDa) Substrate Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455...cell membrane and deliver the substrate; however, secreted luciferase or the use of alternative substrate...

  3. Mammalian RNAi Tools

    Type
    Collection
    ...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...

  5. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...plot. In this post, I’ll walk you through how to interpret the antibody readout on a flow plot.  Note that...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...

  6. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application...attenuated, nef-deleted HIV-1 strains in vivo. Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-...

  7. How Addgene Creates Content

    Type
    Blog Post
    ...quickly." Final design (right). Immediate and immense regret of the graphic designer not shown.   However... and knowledge changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid...

  9. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...website resources that best match a user’s query, and return the matching resources in an order that makes sense...expose as filters and designed the search engine to return only the filters that are relevant to a specific...

  10. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates...

  11. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ... and sequence displays are now much easier to interpret and analyze at a glance. For a quick look at just...“Analyze Sequence” button. The maps hosted here retain all the features shown in the static maps discussed...

  12. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...few commonly available different fragments that retain antigen binding (Figure 2):  F(ab')2 - These fragments...fragments are produced by proteolytic digestion that retains an antibody’s disulfide bonds within the hinge ...

  13. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have...to permanently induce expression? Check out our Retro expression vector and Lenti expression vector. ...

  14. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...demonstrate SEND as a modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA...

  15. RNA Extraction Without A Kit

    Type
    Blog Post
    ... and RNA is retained in the top, aqueous layer, while DNA and other proteins are retained in the interphase...

  16. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...plan for a career outside academia (that would be pretty much all scientists), a LinkedIn profile is absolutely...rules of good resume writing. Include examples of concrete accomplishments in both the technical and “plus...

  18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such as human growth...microenvironments! Take myoepithelial cells for instance. They retard tumor formation by inhibiting cell growth, invasion...

  20. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...fiction story that doesn’t require any detailed retention of information or formulation of new knowledge...mental bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise...
Showing: 241 - 260 of 582 results