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We narrowed to 612 results for: RET

Showing: 241 - 260 of 612 results
  1. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post
    ..., you’ll first need to learn a little bit of theoretical background on the different parameters that your... tumor sample You made it! So much about the theoretical background. Let’s dive into the praxis of gating...
  2. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...researchers realized they needed specific tools to interpret the roles of individual integrins. The Ha lab ...
  3. CRISPR 101: Homology Directed Repair

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    Blog Post
    ...This means all newly synthesized sequences are retained by the original damaged DNA molecule. Unlike in...invasive strand is displaced from the template and returned to the processed end of the non-invading strand...
  4. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post
    ...expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently...%}   Interfering in the future While RNAi has retreated a bit to the background, it remains a solid option...
  5. Filming Science Videos in the Age of Social Distancing

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    Blog Post
    ...composition.  So back to the drawing board.  Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing...acquire gear that’s only good once. Even when things return to relative normalcy, I’ll be incorporating that...
  6. Transferable Skills Guide: Time Management

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    Blog Post
    ...entire experiment, but you’ll have to spend the entirety of each day doing the experiment. The second way...during my day according to my priorities, I have a pretty good idea about the specific things I’ll be doing...
  7. Plasmids 101: Dimers and Multimers

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    Blog Post
    ...that affect the frequency of multimerization. Theoretically, as long as all the plasmid features remain ... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...
  8. Antibodies 101: Validation

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    Blog Post
    ...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample...
  9. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post
    ...good solution. The process of error-prone PCR is pretty much exactly what it sounds like — a PCR, but with...antibodies that target families of cell surface and secreted proteins (glypicans, integrins, and more coming...
  10. A Guide to Getting Started in Undergrad Research

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    Blog Post
    ... try research? Personally, I have always been a pretty curious person. I like to ask questions and think...that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots you don't take” quote...
  11. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no....
  12. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post
    ... proper behavioral readout that allows you to interpret the results of your experiment. Like many things...optogenetics has already been proven. Therefore, a concrete, well defined, and robust behavioral output is...
  13. A Needle in a Base-Stack: Cas9 Structural Biology

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    Blog Post
    .... Other techniques, like electron microscopy and FRET, can offer more information on these regions.   ...SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo et al., 2022). ...
  14. CRISPR 101: Cytosine Transversion Editors

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    Blog Post
    ...editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding. Pro tip! In CRISPR...editors (calling them GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing...
  15. Deep Dive: qPCR

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    Blog Post
    ...when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in a single well.... Epub 2009 Feb 26. PMID: 19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. ...
Showing: 241 - 260 of 612 results