Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 16 results for: RET

Showing: 1 - 16 of 16 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...7.0. Pro-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only...complete media and mix. Seed all cells in 1 CS2. Return to incubator for 48 h. Trypsinize and resuspend...CS2 into one CS5 with a total volume of 850 mL. Return to the incubator for 24 h–36 h. Proceed with transfection...new one before putting the CS5 in the incubator. Return the CS5 to the incubator for 96 h. This incubation...Distribute the media evenly between the layers. Return the CS5 to the incubator for ~72 h. Harvest cells...minutes on ice between each pulse, 50% amplitude. Return to ice between each round of sonication to avoid...

  2. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ... not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can be stored at...prepare your plasmid DNA. In the morning, when you retrieve your liquid bacterial culture, take 500 μL of ...

  3. Using a Light Microscope Protocol

    Type
    Protocol
    ...resolution (the ability to distinguish between two discrete objects). Figure 1 depicts an image of a compound... sample information so that you or others can interpret your images in the future. Conclusion Like any...

  4. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...results in cell populations that are more likely to retain stable transgene expression. Other methods of generating...well in sparse/individual cultures due to lack of secreted growth factors, so not every cell type will be...

  5. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...position 15-19. Avoid targeting introns. Avoid stretches of 4 or more nucleotide repeats, especially repeated...Incubate for 20-30 minutes at room temperature. g. Retrieve HEK-293T cells from incubator. The cells should...

  6. Protocol - How to Design Primers

    Type
    Protocol
    ...end, and the 5’ end of the primer usually has stretches of several nucleotides. Also, both of the 3’ ends...

  8. Ligation Independent Cloning

    Type
    Protocol
    ...cloning scars"). A “stuffer” sequence allows for electrophoretic separation of linearized vector from the reaction...

  10. General Transfection

    Type
    Protocol
    ...first. Pro-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only...

  14. Pouring LB Agar Plates

    Type
    Protocol
    ...manufacturer to make sure this is true in your case. Retrieve your molten agar mix from the autoclave. Pro-Tip...
Showing: 1 - 16 of 16 results