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We narrowed to 741 results for: REV

Showing: 241 - 260 of 741 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...modification was impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger...silent, they could identify the target genes of previously uncharacterized enhancers. The popular TALEN-...CRISPR activators and repressors are thought to be reversible once the effector is inactivated/removed from...KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced methylation...

  2. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...protein. Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem.67.1.509  More...Protein (GFP) and other fluorescent proteins revolutionized live-cell protein detection, their bulk (~27...which lead to cystic fibrosis, one of the most prevalent inherited diseases. The authors investigated the...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...rAAVs, can sound almost too good to be true! In a previous post, we covered systemic capsids, which allow...1, rAAV2/2, and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish...(also known as AAVrg) We’ve covered AAVrg in a previous blog post, but to summarize briefly: AAVrg is ...variables, so be sure to check the literature for previous work in your experimental system. Table 3: AAV...

  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... activation and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive...the LOV domain blocks the enzyme binding domain preventing downstream signaling pathways. Light exposure...optimize the intensities of light sources used to prevent activation of both tools with each light source....  Finding the right information When using a previously published system in your own work, you’ll need...

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...sugar-containing molecules in molecular biology labs (for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology... have visible colonies growing on the membrane wherever an E. coli colony expressing a functional binding...  References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications for old resources...Bases in Bacteria and Their Viruses." Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David...

  6. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...everything there is to know is a strange and exciting revelation. Science in any field can generally be applied...or not. Choose wisely This point ties into the previous one too. I would recommend applying for multiple...factors that can make or break a lab experience. Previous Addgene blogs have discussed the process that ... but I’ve learned the value in finding mentors wherever I go. This story by Dr. Erika Moore in Science...

  7. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...yeast, flies, and even plants! In this blog we will review common uses of MoClo and the kits Addgene offers...the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015). This kit...2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression, ...Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American...

  8. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...suitable for targeting FRET acceptors to proteins (reviewed in [Yan & Bruchez, 2015]). To get you started,...HTRF: a technology tailored for drug discovery–a review of theoretical aspects and recent applications....energy transfer as a spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID...

  9. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...of plasmid prep do you need? As we covered in a previous post on plasmid preps, not all plasmid preps are...purification kits make it easier than others to prevent quality issues. For example, some kits reduce the...weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore... high level of consistency between lots, then reviewing their quality assurance documentation can help...

  10. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...case letter to the beginning of their insert abbreviation to specify what species it is. Example: ‘h’ ...amino acid mutation is to list the one letter abbreviation of the wild type amino acid immediately followed... Methionine (Met) followed by the one letter abbreviation of the mutated amino acid currently at that ...

  11. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...Annals of Internal Medicine published systematic reviews on an agent used to promote bone growth based on... open access as it does about the state of peer review. 8)     That same issue of Science also included...showing overwhelming support for open access to peer-reviewed journal publications, although many don’t necessarily...

  12. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...recent conversations with three lab heads have revealed that a combination of hard work, determination...How do you manage work-life balance? Tom Ellis reveals “I don't. Ask me again in five years and hopefully...Scientists Maintain a Good Work-Life Balance? Read the Previous Posts in this Series Advice for Choosing a Research...

  13. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...be completed and reviewed. The timeline therefore will be as follows:  Request review: 1–2 days Prep and...µL (5 mL).  Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...

  14. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ... et al. "PP09. 1–2352: Systematic review of incidence and prevalence of spinal muscular atrophy (SMA)....S65. Additional Resources on the Addgene Blog Previous BeHeard Awardees Other Efforts to Support Rare...

  15. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...Utility. Annual Review of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-...Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to receptors...

  16. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...you’re ready. Plus, what better addition to peer review than having your plasmids sequenced and openly ...moved on to their next position. We do routinely review deposits that are left in the hold status and reach...depositing plasmids with Addgene! We'll continue to review our policies to make sure they meet our goals, ...

  18. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... Need a quick review of the different immune responses? We got you! You can also review antibodies in ...Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent effector...

  19. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...analyses with the relevant figures from a literature review. No more hunting through a whole folder of papers...might include a confirmatory image to show that a previously used reagent performs well, or you might publish... one such research product which can convince reviewers of your expertise and insight. We recommend sharing...

  20. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... a single point mutation in the PAM sequence, preventing the Cas nucleases from re-identifying it (3)..... Addgene has empowered researchers to harness previous experimental successes and further develop the... FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature Reviews Microbiology. 2011 Jun; 9, 467-477. Bondy-Denomy...
Showing: 241 - 260 of 741 results