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We narrowed to 782 results for: SCR;

Showing: 241 - 260 of 782 results

  1. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...characteristic radiance for some time – there are records describing them dating back to the first century A.D. – ...glowing protein could be used to measure gene transcription, as well as to track protein localization. And...mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered many...

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...ability to direct enzymes – from nucleases to transcription factors – to specific sequences of DNA has become...proteins The tool she and her colleagues led by Zhao described and demonstrated in a report published in The ...of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014...

  3. Editor's Choice, September 2016

    Type
    Blog Post
    ..., P1 phage. 2) The post describes an ingenious but simple technique to screen for proteins that could ...Jessica Sacher from the University of Alberta and describes a technique you can use to find phage proteins...

  4. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ... discusses some of the programs SITN runs and describes some of the more popular topics from the Science...telling. Chapter 5: The SITN Blog - Vini and Amy describe how the SITN blog is a source of scientific information...underwater? Chapter 8: Work-Life Balance - Vini describes how she balances working in the lab and working...

  5. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ... paired DSBs. This method allows for deletion screening by conventional PCR. Biallelically disrupted cells...transfected cells via FACS, primer validation, and screening by PCR for biallelic deletion clones:    ...video? The Orkin and Bauer lab's related paper describes their methods for CRISPR design, cloning, and ...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, ...former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety...3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. ...

  7. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...

  8. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...positive selection, negative selection, and/or screening. We’re focusing on positive and negative selection...in this blog post, but don’t worry, we’ll cover screens in a future post. Positive selection: Cells that...with or without your inserted DNA) and further screening will help you determine if the plasmid backbone...

  9. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

  10. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...plasmid with its elements described below.   Vector Element Description Origin of Replication ...for further study. Promoter Region Drives transcription of the target gene. Vital component for expression...

  11. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...camelids too, though not just for the reasons the UN describes. In honor of the Camelid family, I wanted to take...of times on this blog over the years and have described how they compare to other antibodies and affinity...sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs in the clinic sdAbs have...

  13. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening libraries ...John Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this Broad...

  14. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...CRISPR/Cas inspired genome editing was initially described by Cong et al. and Mali et al. in January 2013...Nine months later, more than 1500 publications describe work to improve the tool’s specificity, orthogonality...orthogonality, and multiplexibility in various species and describe an assortment of applications. Despite the ubiquity...

  15. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...(WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when transcribed which ... of RNA and RNA translation, and promotes RNA transcript longevity. The polyA is located at the end of...

  16. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before being...depending on the class of virus. RNA genomes can be transcribed into mRNA, directly translated into proteins,...

  17. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests...each day brings in new and surprisingly diverse manuscripts. I work with people, nurturing networks, as much...Career Decisions Check Out Our Career Resources Subscribe to Career Blog Posts Resources at Addgene.org...

  18. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...large-scale functional screen in bacteria. Sensor variants were then refined by a secondary screen in rat hippocampal...developed and published in 2010 for visualizing the transcription factor cAMP-responsive element-binding protein...

  19. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...to keep the audience engaged through a computer screen. For the Reproducibility for Everyone Workshop,...author of the poster,” he says.Viewing a poster on a screen is challenging, requiring you to zoom in to different... their poster, eliminating the need to zoom and scroll around the poster (Reshef et al., 2020).The Twitter...

  20. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ..., lengthy genetic payloads and insufficient transcription on the array have limited the scalability and...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...
Showing: 241 - 260 of 782 results