We narrowed to 532 results for: SHI

...you have any particular tips or tricks for those wishing to purchase and use the SpyLigase system for the...others to Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation...

...intracellular pH are required in order to determine the relationships between pH changes and cellular events. To overcome...optogenetic tool for activating endogenous genes Moritoshi Sato's Lab has engineered CRISPR-Cas9 to create..., the Cohen lab further modified sdChr- a blue-shifted channelrhodpson from freshwater green alga identified...Rowland, et al. Cell. 2014. PubMed PMID: 25416943 Shibata, et al. J Biol Chem. 2008. PubMed PMID: 18442980...

...genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all mouse genes with 10 gRNAs...of ready-to-use AAV9 and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! ...

...better with some variety in their job. Good relationships with clients and colleagues Sometimes this means...scientists) Are self-directed, but need precise leadership and support from their superiors (beware of not...

...writing, look into a AAAS Mass Media Fellowship or maybe an internship at a place like Science News. Ask ...

...changes in the AraC dimer. In doing so, the dimer shifts from one that represses transcription to one that... plate containing the pattern of the image, and shined blue light over the plate. Areas on the plate that...

...coronavirus-related deposits. Our valiant lab and shipping staff agreed to come in and start getting plasmid...plasmid deposits into the collection and to start shipping. They created the best safety protocols they could...

...fluorescent protein fluorescing green. Image from Shin et al., 2019. Looking for an alternative to intercalating... in real-time. Find plasmids expressing tTALE-FP Shin et al., Sci. Rep. 2019. https://doi.org/10.1038/...

...The Ohio State University in Columbus, Ohio, ABRC ships more than 100,000 samples to researchers and educators... through our outreach efforts. Our education partnerships have grown to include local school districts...

...individual sections of the paper (see figures). After finishing my section-specific comments, I re-read what I...of tools for data analysis/visualization using R/Shiny. She is passionate about open science, BIG data ...

... like you What is different about academia? Publishing in peer reviewed journals is absolutely necessary... important first step. Developing mentoring relationships, especially with peer mentors, to discuss your...

... is: could NgAgo replace CRISPR? Such a drastic shift won’t happen overnight, but there are a few reasons...impaired NgAgo activity, with three mismatches abolishing activity. One-guide faithful NgAgo guide loading...

...become something tangible.  When you think of entrepreneurship in the 21st century, who do you think of?  ...internal operations and building external strategic partnerships with universities, companies, hospital institutions...

...sensors are always bright, they change color as they shift from "off" to "on" in the presence of calcium. This...resulted in the construction of redCALWY-1, a red-shifted FRET sensor for zinc using variants of mOrange2...

...researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...grant? A patent? It is never too early to discuss authorship and rights for anything that could be generated...

...yeast, as well as mammalian and human cell lines (Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki...SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID: 30166441...

...Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University of Baroda, India  Devarshi Gajjar has 10 years...

..., and how we are using this technique in our partnership with Addgene to sequence large numbers of plasmids...Addgene produces plates of plasmid DNA, they are shipped to seqWell, where they are prepared as plexWell...

...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...

...dependent on the manager to succeed. More people in leadership roles leads to more ideas, initiative and creative...Professional Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through...