We narrowed to 665 results for: SIM

...transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust strategy uses a ...tail. Compared to the opaque mouse embryo, where similar processes occur over days inside the mother, the... Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2...

...transfected into human cells. From this result one could simply conclude that the shRNA didn’t work, as the expression...level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated... controls are negative controls that produce a similar product, but do not target an endogenous gene in...

...efficiently trawled their own data? A distinct (perhaps similarly impoverished lab) might apply different or novel...although it might be argued various authors have simply chosen to submit elsewhere). The majority of the...types? And how long should data be archived? Simplifying data access Some of the above mentioned issues...

...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...expression mRNA Mammalian expression promoter from the simian vacuolating virus 40 Constitutive  May include ...

...confrontational situations with others because you simply cannot see things from their point of view and ...herself to the nearby village (and to the reader) by simply listening. Chances are, you know someone like this...how previous iterations of those teams resolved similar issues in the past, or how completely different...

...Magic Pocket Cinema Camera 4K. It’s wonderful and simple to use, but when you need to juggle filming and...perfect height (Fig. 2). We recorded video using a simple, external video recorder and a mic clipped to her...video conferencing software. Interviewees could simply boot up Zoom and record themselves talking to their...

...fastest test that has been developed so far.  A similar method has been optimized to detect the SARS-CoV...and will not give a positive result for highly similar viruses. Unlike Cas12a, Cas13a does not require...illuminator or UV light illuminator. Approach: Highly similar to the SHERLOCK diagnostic test, this test varies...

...edit your gene of choice. These vectors are very similar, both with polylinkers for the addition of gene-specific...at once. Therefore, applications that require simultaneous modification of all alleles of a gene, or which...which require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based...

...a miniaturized version of Spinach that retains similar levels of fluorescence when bound to DFHBI despite...of artificial guide RNAs that can be used to simultaneously target cellular RNA to specific intracellular... Broccoli are derived from Broccoli, and share similar properties to Broccoli despite binding to a different...

...the immune response. The second strategy is the simple removal of the HIV-1 genome from infected cells...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...

...postdocs. Unfortunately, even when universities have simple postdoc numbers sorted out there can be gaps in...important take-away from this session was the idea that simply satisfying a percent requirement for diversity ...difficult question but not one that should be abandoned simply because it is difficult and is key to the continued...

...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H. Cell. 2010 Oct 1;143(1):134-44. ...Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar;8(3):253-9. doi: 10.1038...

...cells? Immunoblotting or simply the western blot, or western, is one of the simplest methods to detect the...could contain unintended cross-reactive proteins. Similarly, if you vary the experimental conditions from ...

...with nothing more than substrate addition and a simple add-mix-measure method. NanoBiT® luciferase activity...from the Promega Plasmid Collection that express similar constructs available at Addgene. Read the original...whole new light!   This post was written by Simon Moe, an Associate Product Marketing Manager at Promega...

...the go-to choice for studying mammalian biology. Similar to any model, mice are not without their problems...manipulate the mouse genome using CRISPR/Cas9. Similar to the human genome, the mouse genome is made up... finally, we have a technology, CRISPR, that is simple in concept, straightforward to use, and robust ...

...I changed jobs. I highly recommend taking on a similar position in your field of interest before you dive... solution attempted was more meetings, but this simply seemed to create more bureaucracy. As with any ...anticipated and there can be a glut of individuals with similar skill sets. This is not to say that all commercial...

... cell from the clinic. The 2nd generation is similar to the first one with the exception that the HIV...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

...pitfalls of accepting alternative facts. Because, similar to scientists, the legacy of each government and..., challenged us. I worried the march would be a simple, unquestioning celebration of science, at a time...research scientist would be a career that could simply cease to exist. But then November hit and panic...

...lysing cells and removing cellular debris look very similar. However, keep in mind that kit components (i.e... Figure 2: Plasmid isolation workflows are similar across kits. Lyse your bacteria to release the ...can be more flexible in this situation as you can simply add more beads to your sample. But, you’ll need...

...likely to express the target at a robust level, similar to that in samples of interest. If you are working...   If your protein of interest is expressed at similar levels in your cell lines, you can consider a cell...transiently overexpressed in CHO cells and displayed similar labeling patterns on different primary blood leukocytes...