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We narrowed to 651 results for: SIM

Showing: 241 - 260 of 651 results

  1. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...a miniaturized version of Spinach that retains similar levels of fluorescence when bound to DFHBI despite...of artificial guide RNAs that can be used to simultaneously target cellular RNA to specific intracellular... Broccoli are derived from Broccoli, and share similar properties to Broccoli despite binding to a different...

  2. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...confrontational situations with others because you simply cannot see things from their point of view and ...herself to the nearby village (and to the reader) by simply listening. Chances are, you know someone like this...how previous iterations of those teams resolved similar issues in the past, or how completely different...

  3. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...Magic Pocket Cinema Camera 4K. It’s wonderful and simple to use, but when you need to juggle filming and...perfect height (Fig. 2). We recorded video using a simple, external video recorder and a mic clipped to her...video conferencing software. Interviewees could simply boot up Zoom and record themselves talking to their...

  4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...the immune response. The second strategy is the simple removal of the HIV-1 genome from infected cells...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...

  5. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...postdocs. Unfortunately, even when universities have simple postdoc numbers sorted out there can be gaps in...important take-away from this session was the idea that simply satisfying a percent requirement for diversity ...difficult question but not one that should be abandoned simply because it is difficult and is key to the continued...

  6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H. Cell. 2010 Oct 1;143(1):134-44. ...Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar;8(3):253-9. doi: 10.1038...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...cells? Immunoblotting or simply the western blot, or western, is one of the simplest methods to detect the...could contain unintended cross-reactive proteins. Similarly, if you vary the experimental conditions from ...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...the go-to choice for studying mammalian biology. Similar to any model, mice are not without their problems...manipulate the mouse genome using CRISPR/Cas9. Similar to the human genome, the mouse genome is made up... finally, we have a technology, CRISPR, that is simple in concept, straightforward to use, and robust ...

  9. Finding Your Perfect Job After University

    Type
    Blog Post
    ...I changed jobs. I highly recommend taking on a similar position in your field of interest before you dive... solution attempted was more meetings, but this simply seemed to create more bureaucracy. As with any ...anticipated and there can be a glut of individuals with similar skill sets. This is not to say that all commercial...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... cell from the clinic. The 2nd generation is similar to the first one with the exception that the HIV...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

  11. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...pitfalls of accepting alternative facts. Because, similar to scientists, the legacy of each government and..., challenged us. I worried the march would be a simple, unquestioning celebration of science, at a time...research scientist would be a career that could simply cease to exist. But then November hit and panic...

  12. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...lysing cells and removing cellular debris look very similar. However, keep in mind that kit components (i.e... Figure 2: Plasmid isolation workflows are similar across kits. Lyse your bacteria to release the ...can be more flexible in this situation as you can simply add more beads to your sample. But, you’ll need...

  13. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...likely to express the target at a robust level, similar to that in samples of interest. If you are working...   If your protein of interest is expressed at similar levels in your cell lines, you can consider a cell...transiently overexpressed in CHO cells and displayed similar labeling patterns on different primary blood leukocytes...

  15. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...protein that enables imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin ...mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA...PhoCl between an enzyme and an inhibitor domain similarly allows for control of enzymatic activity. In addition...

  16. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ... of the best ways to learn about a new field is simply to know where to look and how to start. There is...  About the author Niko McCarty is the editor of Asimov Press and is designing a genetic engineering curriculum...

  17. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...structures demonstrating “the structure is strikingly similar to the design model at atomic resolution” (Kuhlman... design is possible. Although Top7 was designed simply to be stable and novel, the principles that created...

  18. Call for Guest Bloggers

    Type
    Blog Post
    ...in the biological sciences and techniques that simplify and expedite research. We recognize, however, ... blog! To sign up to write for the Addgene blog simply email us. We can’t guarantee that all those who...

  20. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...application. The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate...can work through it at your own pace. To submit, simply click "Contribute Data."    Figure 6: Contributing...
Showing: 241 - 260 of 651 results