We narrowed to 466 results for: Sele
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TypeBlog Post...IRES-Puro marker to facilitate stable cell clone selection. Expression is driven by a fairly strong EF1alpha...
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FPbase: A new community-editable fluorescent protein database
TypeBlog Post...research. Any microscopist will tell you that selection of a fluorescent probe (be it an organic dye or... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...and end repair Adapter Ligation Clean up/Size selection Amplification Clean up I found this kit easy... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...Citation Formatter Just enter the DOI of a paper and select your desired formatting style. This tool is great... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...off-target mutations, scientists are using sgRNA for selection and optimization. In addition, the current work... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...characterizing iGluSnFR in vitro, showing that it is selectively activated by glutamate, and tweaking it to improve... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...population can affect drug and dose response. Selective serotonin reuptake inhibitors are frequently used... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...titering, testing, and providing lentiviral preps of select lentiviral vectors from the repository for you.... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with... -
Pushing the Limits of DNA Assembly
TypeBlog Post...reaction. DNA fragments with the desired promoter, selectable marker, and target gene, for example, are amplified... -
5 Great Apps for Lab Life
TypeBlog Post...reason. It contains all twenty amino acids plus selenocysteine and pyrrolysine. I have found myself wishing... -
Antibody Guide
TypeCollection...nuclei, AF488, AF555, and AF647 Antibody Selection Selecting the right antibody for your experiment is...section to help you select antibodies for your approach. Primary antibodies To select an antibody, first...Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents are molecular tools...mid-forties. When designing a multiplex assay, selection of the fluorophores used is important. It is best...appropriate lasers and filters for each fluorophore you select. Suggested panels for small multiplex experiments... you may either purchase several to compare, or select one based on price, shipping availability, ease... called secondaries, are usually much easier to select. There are three main considerations. What is the... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...GFP- population ends, you can draw a gate which selects anything to the right of it, marking it as GFP+... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...cloning X gene, an SV40 promoter, and Neomycin selection. Table 1: Gamma-retrovirus glossary Plasmid... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...requirements As we all know, a Cas guided by a gRNA is useless without an adjacent PAM or protospacer-flanking... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...almost endless possibilities for researchers, from selective protein ligation through introducing ncAAs with... -
Viral Vectors 101: Viral Applications
TypeBlog Post...constitutive), your gene of interest, and often a selectable marker. This vector can express your construct... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post... samples arrive, each group of plates will have select samples prepared and screened by NGS to spot check... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...binding screens, peptide arrays, and phage display selections. In addition to these constructs, the Sidhu lab... -
Antibodies 101: Isotypes
TypeBlog Post...useful to know the isotype of an antibody so you can select an appropriate isotype control. An isotype control...