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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 472 results for: Sele

Showing: 241 - 260 of 472 results

  2. Tetracycline Inducible Expression

    Type
    Collection
    ... of shRNA with puromycin selection. See Plasmid #21916 for neomycin selection. TetR H1-2O2 Dmitri Wiederschain...puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection. TetR ...vector with blasticidin selection. See Plasmid #26730 for hygromycin selection. rtTA3 Eric Campeau 128061...article ( Kowarz et al., 2015 ) for additional selection markers. rtTA-Advanced Tight TRE Eric Kowarz 96930...expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE David Root 100521 pCW57.1...vector for repressible expression with blasticidin selection. *Note: this vector contains an insert (MAT2A)...for CMV-driven rtTA with EGFP and Blasticidin selection rtTA-Advanced Mikhail Alexeyev 17492 pLenti CMV...

  3. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically...strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase.... ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

  4. Antibody Guide

    Type
    Collection
    ...nuclei, AF488, AF555, and AF647 Antibody Selection Selecting the right antibody for your experiment is...section to help you select antibodies for your approach. Primary antibodies To select an antibody, first...Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents are molecular tools...mid-forties. When designing a multiplex assay, selection of the fluorophores used is important. It is best...appropriate lasers and filters for each fluorophore you select. Suggested panels for small multiplex experiments... you may either purchase several to compare, or select one based on price, shipping availability, ease... called secondaries, are usually much easier to select. There are three main considerations. What is the...

  8. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...reaction. DNA fragments with the desired promoter, selectable marker, and target gene, for example, are amplified...

  15. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...reason. It contains all twenty amino acids plus selenocysteine and pyrrolysine. I have found myself wishing...

  16. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI...Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9...Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6...
Showing: 241 - 260 of 472 results