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Showing: 241 - 260 of 612 results

  3. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Estimate of time required: ~3 h Workflow Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis: 30 min Equipment...load the standards and samples onto the qPCR plate Set up and load the 96-well plate: Load 5 μL of each ...repeat 39x from step 3 / melt curve Example of plate set-up: 1 2 3 4 5 6 7 8 A 1.00 x 10 9 1.00 x 10 8 1.00...

  4. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Clamp stand and clamps... NanoDrop Spectrophotometer using the A280 IgG setting. Combine all eluates with measurable protein. Determine... NanoDrop Spectrophotometer using the A280 IgG setting. If the concentration of the pooled sample is above...

  8. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... a high multiplicity of infection, because this setup allows for experimental replicates for each of the...labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells in a population...

  11. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered and ...

  16. An Addgene Summer Internship

    Type
    Blog Post
    ...majoring in biochemistry in college and play the drum set as their personal instrument. They are the oldest...options from the Addgene catalog. These were a miR30 cassette, Syntaxin3, and osa-miR528. My manager, Katherine...

  17. The MTA at Addgene

    Type
    Blog Post
    ... as to how the shared reagents should be used. Setting clear expectations from the beginning makes the...
Showing: 241 - 260 of 612 results