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...serological pipettes from Corning®, Falcon®, or Axygen®, set up a bin or box in your tissue culture room for the...but your institute may still be interested as it offsets the cost of regular waste services. Learn about...

...you could see yourself taking? If so, consider setting up an informational interview with them to learn...

...Bertuzzi welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group...

...technology that can be used to quickly barcode a set of DNA sequences. In this post, Alan Wong (formerly...

...plasmids get their name? In 1952, Joshua Lederberg set out to clarify the classification of these cytoplasmic...

... lab members as possible. Try to get them in a setting out of the lab (coffee, lunch or beer) and in 1...

...research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository....

... the experimental setup and objectives. Most systems can only excite and read a set number of excitation...this is limited by the filter cubes and filtering settings of the equipment. Systematic evaluation of commercially...

... LDR has also been extended to multiplexed probe sets that allow the simultaneous interrogation of multiple...SNP sites (15). The related LCR technique uses two sets of probes to achieve exponential amplification. ...

...majoring in biochemistry in college and play the drum set as their personal instrument. They are the oldest...options from the Addgene catalog. These were a miR30 cassette, Syntaxin3, and osa-miR528. My manager, Katherine...

... as to how the shared reagents should be used. Setting clear expectations from the beginning makes the...

...easily achieved with alternative methods. Ready, set, transduce! Making virus can sound daunting, but ...

... no idea where to start. Thankfully, my managers set aside time for me to complete an online social media...

...Preventable errors in the design of cloning strategies set experiments back days or even weeks. Primer design...

...beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... a high multiplicity of infection, because this setup allows for experimental replicates for each of the...labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells in a population...

...Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Human Gene Therapy Methods 30:195–205 . https...vector genomes as containing just the expression cassette that we want, it also includes a small amount ...

...break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered and ...

...percentage of the recombined vector backbone.   Ready, set, grow! Our 5 Tips for Troubleshooting Viral Transductions...

...establish that schedule. There’s also tips for setting writing goals, tracking progress, and starting ...

...other epitopes and scaffolds to build a more robust set of frankenbodies. References Ewert, S., Honegger...