Plasmids 101: Screens vs. Selections
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...and consider these questions to save yourself a headache later on.
Enhancer and suppressor screens
These...
We narrowed to 345 results for: ada
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Hot Plasmids - February 2022
TypeBlog Post...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...Nature 560.7717 (2018): 248. PubMed PMID: 30069054. Sadanand, Saheli. "EvolvR-ing to targeted mutagenesis."... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post... dsRNAs target specific mRNA transcripts for degradation. Despite how easy these experiments are to physically... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al. You can also search by ... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...that can make vector propagation and sequencing a headache. How do I maintain ITR integrity and prevent unwanted... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post... samples may allow for better interpretation. Priyamvada Prathima has a research background in protein... -
Viral Vectors 101: Biosensors
TypeBlog Post...measured GFP levels and converted that signal into a readable value. Types of biosensors So now that we know... -
Viral Vectors 101: Viral Applications
TypeBlog Post...which target complementary mRNA sequence for degradation. Viral introduction of shRNA plasmids can induce... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...’re not at your computer. Maps, sequences, & downloadable .gb & .dna files Once you’ve identified a plasmid... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...Repression and Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...ardor and attended to with diligence.” - Abigail Adams, 1780 There are potential mentors all around you... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...provided by the major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but... -
Visualizing Protein Turnover In Situ
TypeBlog Post...the combined effect of protein synthesis and degradation. These processes control the abundance of proteins... -
Summer SciComm: Preprints
TypeBlog Post...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...