Kit Free RNA Extraction
Type
Protocol
...Isopropanol or Lithium Chloride. This protocol was adapted from Chomczynski P and Sacchi N, 2006 and TRIzol...is not as stable as DNA and is susceptible to degradation by heat, RNases, and other enzymes. *Pro-Tip*...reagents cold and work quickly to reduce RNA degradation. *Pro-Tip* Keep work area, equipment, and reagents...Having your samples spin at 4°C helps reduce RNA degradation. If you don’t have access to a refrigerated centrifuge... your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw cycles. *Pro-Tip...