We narrowed to 333 results for: cki

1. Antibodies 101: Affinity Reagents

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   Blog Post

   ...nanobodies, are derived from a camelid antibody lacking a light chain, called a heavy chain antibody (Muyldermans...

2. Lighting Up Cell Signaling with Photoswitchable Kinases

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   ... interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces green fluorescence...

3. New Tool for Lineage Tracing: The ClonTracer Library

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   ...their own work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and...

4. Antibodies 101: Stripping and Reprobing Western Blots

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   ...ll want to check for the primary antibody by re-blocking your membrane and re-incubating with your secondary...

5. Progress Towards a PAM-Free CRISPR

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   ...at least has looser or different requirements. Luckily, there are some available.   Cas proteins that ...

6. Hot Plasmids February 2024

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   ...was delivered to brain endothelial cells in mice lacking LY6A using AAV1-X1 (Chen et al., 2023); then, when...

7. Antibodies 101: Choosing the Right Isotype

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   ...References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ...process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...

9. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   ...or their translation disrupted through physical blocking of the translation machinery. In the Sánchez lab...

10. Lab Automation at Addgene

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    ...protocol on the Biomek FxP is pretty simple. By clicking a few buttons you can quickly tell the Biomek ...

11. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...Charlton J, Mattei AL et al (2018). Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun...

12. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...improve genetically-encoded research tools, it is shocking that the plasmids and sequences for antibodies...

13. Hot Plasmids - October 2020

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    ... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

14. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without...

15. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    ...different and potentially more catchy, but it can be trickier to make figures fit nicely into circular shapes...

16. Corralling and Cornering the Goldilocks Antibody

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    ...enable scientists to have an intuitive guide for checking their staining and neuroanatomy without the fear...

17. Tips for Technical Support Calls

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    ...customers within 24 hours, and we’re pretty good about sticking to that. If you email us at noon and then call...

18. Plasmids 101: FLEx Vectors

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    ... 2003 PubMed PMID: 12665802. 2. Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of ...

19. Tips for Titering Your Lentiviral Preps

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    ...original plasmid which can over-inflate results. A packing vector deficient in reverse transcriptase will ...

20. Gaining Leadership Skills Volunteering at a Professional Organization

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    ...resulted in actionable tasks and I made a point of sticking to deadlines and performing the work I agreed ...