We narrowed to 334 results for: cki;

1. Addgene: The First Twenty Years

   Type

   Blog Post

   ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...

2. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   ...surface display vectors to improve expression and trafficking of these biosensors.   Figure 1: Schematic...

3. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   ...between them, could affect the properties or trafficking of the fusion protein (Kimple et al., 2013). ...

4. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   ...rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However...

5. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   Blog Post

   ... postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...

6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   ...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and...

7. New Tool for Lineage Tracing: The ClonTracer Library

   Type

   Blog Post

   ...their own work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and...

8. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   ...or their translation disrupted through physical blocking of the translation machinery. In the Sánchez lab...

9. Lab Automation at Addgene

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   Blog Post

   ...protocol on the Biomek FxP is pretty simple. By clicking a few buttons you can quickly tell the Biomek ...

10. Hot Plasmids - October 2020

    Type

    Blog Post

    ... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

11. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    ...activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without...

12. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    ...different and potentially more catchy, but it can be trickier to make figures fit nicely into circular shapes...

13. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    ...ll want to check for the primary antibody by re-blocking your membrane and re-incubating with your secondary...

14. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

15. Corralling and Cornering the Goldilocks Antibody

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    Blog Post

    ...enable scientists to have an intuitive guide for checking their staining and neuroanatomy without the fear...

16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    ...Charlton J, Mattei AL et al (2018). Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun...

17. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post

    ...improve genetically-encoded research tools, it is shocking that the plasmids and sequences for antibodies...

18. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    ...at least has looser or different requirements. Luckily, there are some available.   Cas proteins that ...

19. Hot Plasmids February 2024

    Type

    Blog Post

    ...was delivered to brain endothelial cells in mice lacking LY6A using AAV1-X1 (Chen et al., 2023); then, when...

20. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    ...process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...