We narrowed to 310 results for: construct

1. Viral Production at Addgene

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   Blog Post

   ...determine recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin contamination in vector ...

2. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ...specifically isolate bacteria containing your constructs of interest. But does it matter which antibiotic...

3. High Complexity Golden Gate Assembly with NEB’s DAD

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   Blog Post

   ... Lohman, G. J. S. (2022). Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly...

4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   Blog Post

   ...  A second application for CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction...

5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

   ...reverse transcriptase, protease, and Bst polymerase constructs from their assay to Addgene.  Drew Endy’s and...

6. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   Blog Post

   ...allowing the screen to be conducted with only two constructs (the other containing dCas9-VP16). Like CRISPRi...

7. Plasmids 101: Codon usage bias

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   Blog Post

   ... different genes without having to create new constructs. However, due to problems such as mismatched ...

8. Designing Your Chalk Talk for the Academic Job Interview

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   Blog Post

   ...competing models. Comparing model A vs. B is more constructive than testing the validity of a single model....

9. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ... Figure 2: (A) Streamlined VchINT expression constructs. (B) High integration efficiencies achieved with...

10. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...spent two years testing and reformulating our AAV construct to optimize performance in our system and yet ...

11. Bioinformatics at Addgene

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    Blog Post

    ...time a new process was added, we progressively constructed the entire workflow in the Nextflow script. With...

12. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post

    ...background signal (especially for some older split-GFP constructs or certain cell environments). Also consider ...

13. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    ...A206K mutation [2]. Addgene has many of these constructs and they are designated mEGFP, mVenus, etc. A...

14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post

    ...deciding where to place an FP within a fusion construct [1,2], i.e. positioning of the FP sequence relative...

15. Plasmids 101: Multicistronic Vectors

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    Blog Post

    ...same multicistronic vector, effectively making a construct that expresses multiple unique genes at equivalent...

16. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ...commonly employed in lentiviral and retroviral constructs and we will discuss these in a future post on...

17. Lentiviral Prep Service

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    Collection

    ...Addgene's viral vector packaging service. Browse constructs currently available in lentiviral format....

18. AAV Packaged on Request

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    Collection

    ...typical titers, though actual yields may vary by construct. Size Quantity Total Volume Minimum Titer Typical...

19. Tetracycline Inducible Expression

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    ...Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control expression of your...Vogelstein Transactivators (tTA or rtTA) Find a construct that expresses the transactivator for your tetracycline...

20. Hot Plasmids Spring 2024

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    Blog Post

    ...produced 90% accurate integration of multiplexed constructs into target genomes and a 10-fold improvement...