...The second problem is fundamental. We built a NOR gate because they are used to create any program you ...that took a spaceship to the moon was built on NOR gates. It’s a fundamental unit. We did demonstrate that...that you can use the CRISPR chemistry for a NOR gate. But there are a couple of problems. You couldn’t ...

... by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin columns...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

...complexes, but a serendipitous discovery led them to investigate the use of a common laboratory chemical, guanidine...gene using Cre recombinase and to generate Nfix-negative neural stem cells. The lab will use plasmids from... delay, result in loss of function or dominant negative NFIX phenotypes. Developing a rapid test for ...

...work well. It is important that your readers can navigate your poster and follow the logical progression...all cases the idea is the same; the reader will navigate the poster from column to column left-to-right...the Addgene blog Check out this blog post on navigating the poster session Find tips to make the most...

...significant risk for aerosol contamination. As most centrifugation has to be done outside of a biosafety cabinet...use aerosol-tight caps, compatible with your centrifugation speed, to cover the centrifuge bins. These ... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ...

...tools in about a week. Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol ... enzymes and reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying...

...flow plot. This will make it difficult to properly gate your desired cell population. By compensating between...your experimental samples, you will first need to gate out your main population and singlets. Then, you...More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry...

...muscle contractions, and the control of voltage gated ion channels all depend on transmitting and sensing...action potentials which are electrical charges propagated through multiple cells to do things like translate...historically come from a variety of sources – voltage-gated potassium channels found in Drosophila to phosphatases...

...digest cuts DNA into smaller pieces, and proximity ligation creates chimeric circles of DNA that are distant...into smaller pieces. Sticky ends created are then ligated together to create loops of DNA. This is key to...there was little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites...

...learned that some of the technologies that helped us navigate a global pandemic can also be the thing that provides...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns... I hope the tips and tricks above can help you navigate it in confidence.   Ashley Bastin is a first ...

...model of the protein. At some point during this interrogation process, they will almost invariably say something...creating physical models of proteins that are being investigated in their labs. Soon after we began working with...gymnastics that this protein must undergo as it interrogates your genome. If you are interested in experiencing...

...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...Laboratory of Molecular Biology. He is currently investigating the molecular biology of synaptic transmission...and plasticity.     Javier García-Nafría is an investigator scientist at the MRC Laboratory of Molecular...

...solution. Proteins and genomic DNA are removed by centrifugation. At this point, the plasmid DNA sample can ...binding DNA, the glass particles are pelleted by centrifugation and washed before DNA is eluted with water ...phosphoric acid for 3 minutes followed by a short centrifugation for a total of four times before a wash with...

...closer look at this new functionality and ways to navigate it, we’ll search for the calcium biosensor, GCaMP... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your... keeps more than half of these. Instead we can navigate first to the CRISPR Resources page: The page ...

...preparation after negative staining. Empty vector particles can be identified after negative staining and ...proteins) as opposed to purified DNA. This may be mitigated by using purified viral DNA as the template instead...

...recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (Enabling Discovery ...experimental species. Discovering ways to culture, propagate, transform, store, and share all of these new ...cephalopods die after they lay their 1 clutch of eggs? Propagation is thus quite difficult. CRISPR genome editing...

...Many CRISPR screens use non-targeting guides as negative controls. Non-targeting guides do not target any...sequence in the genome, and are commonly used as negative controls for genome-wide CRISPR screens; and 3... Less significant p-values lead to more false-negatives results (i.e. failing to identify a gene that’...

...of Research, our volunteers have been helping to gather information for our FLSA and postdocs resource ...points on this salary range. We have been able to gather data on changes to salary for some of the institutions...advocating for and with junior scientists, to help them navigate the scientific enterprise and promote their voices...

...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...latent HIV reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount...effects were detected. Kaminski et al. observed no negative effects on cell viability, cell cycle progression...would make it easier for an “escape” virus to propagate and infect adjacent cells. It will be important...