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We narrowed to 535 results for: genome

Showing: 241 - 260 of 535 results

  2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...samples in our queue such as viral samples for Viral Genome Sequencing (VGS). QC is performed throughout the...yield sufficient data to capture the entire viral genome. The full details of that workflow and its analysis...Platt RJ, Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional ...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...sensors, our approach uses portions of the pathogen’s genome as unique barcodes to identify samples containing... Of the 48 sensors, targeting 24 regions of the genome, we have deposited the best performing sensors ...encoding corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these... Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous Viruses Resources...

  4. Editor's Choice, September 2016

    Type
    Blog Post
    ...useful to your colleagues. Technique: Probe Phage Genomes for Host Binding Proteins & Running for Rare Disease...this month. The first, "Technique: Probe Phage Genomes for Host Binding Proteins," was contributed by ...

  5. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...desired genetic modification integrates into the genome (Figure 3). Here, the chromoprotein is expressed...tsPurple) color. Though the toxin is integrated in the genome, it is not expressed as it is under the control...for the Scarless Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16)...

  6. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...RABV, these sequences are not copied into viral genome, so viral progeny will not necessarily express ...can be achieved by modifying the wild-type RABV genome, which encodes a single envelope protein called...incorporated into the viral envelope (but not the viral genome). Nascent G protein-coated RABV is then released...

  9. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...these patients suffer any less acutely.  The Rare Genomics Institute works to help those who suffer from ...disease cures. Through their BeHEARD Award, the Rare Genomics Institute partners with companies to provide technological...

  10. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...bacteria that can incorporate foreign DNA into plant genomes. Eukaryotes are a bit more complicated. Yeast vectors...the plasmid is incorporated into the host cell’s genome, so no special sequence is needed to retain the...remains episomal, meaning it stays separate from the genome. One option for episomal vectors is to include ...

  11. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ... Dotson.  Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional ...screening, and validation of CRISPR libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated... OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional ...

  12. Hot Plasmids - August 2020

    Type
    Blog Post
    ...dCas-VPR repressor are introduced into the hPSC genome using AAV1 and contain an EGFP reporter. Expression...The multi-gRNA piggyBac vector integrates into genomic DNA with the help of the piggyBa transposase. Image... and AAV donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 ...

  13. DNA Purification Without a Kit

    Type
    Blog Post
    ...proteins and genomic DNA to precipitate. Plasmid DNA is free in solution. Proteins and genomic DNA are removed... on the type of DNA you’re purifying (plasmid, genomic, or DNA fragments from agarose).    In this article...

  14. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...considered an authority in this area. What is genome engineering? How does CRISPR work? This section...that, like computers and the internet before it, genome engineering technology is moving along much faster...examples of how understanding genetics is so timely.” Genome engineering, and many other technologies and fields...

  15. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...to introduce reporters throughout an organism’s genome. These reporters have historically been used extensively...Examples of promoter, enhancer, and gene traps at a genomic locus.    Choosing a trap At first glance...

  16. RUBY-Red Siliques

    Type
    Blog Post
    ...events likely due to insertional effects in the genome. (Bottom panel, right) A highly expressing RUBY...Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation...

  17. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...p-element, random or targeted, integration into the fly genome and are under the Gal4 inducible UAS promoter. ...From making transgenic animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc...

  18. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...efficiency using XMAS-TREE-based methods at various genomic loci in four different categories.     Brookhouser...resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data...Cas13Y, two compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered...

  19. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...carries the transgene of interest (aka the viral genome) and the other contains the bacterial selection...paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...
Showing: 241 - 260 of 535 results