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We narrowed to 12 results for: genome

Showing: 1 - 12 of 12 results

  1. CRISPR Guide

    Type
    Guide
    ...that defines the genomic target to be modified. You can therefore change the genomic target of the Cas...of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide...ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-...not permanently modify the genomic DNA. dCas9 has also been used for genome-wide screens to activate or...visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify Genomic Regions ...RNAs), and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...associated with a genomic region of interest in vivo. Figure 12: Overview of purifying genomic regions using...

  2. Lentiviral Vector Guide

    Type
    Guide
    ...will have the lentiviral genome and the genetic information encoded by that genome. Antibiotic resistance...collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient way to determine...characterized by their ability to integrate their genomes into hosts in order to continue their lifecycle...gamma-retroviruses fall under the Retroviridae family. The genome of viruses in this family is made of RNA. This ...transcribed into DNA before being integrated in the genome of a host. Going from RNA to DNA is the reverse...use of “retro”. Lentiviruses have more complex genomes, containing packaging genes and accessory genes...gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from 8–10 kb, ...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... the gamma-retroviral genome and the genetic information encoded by that genome. Antibiotic resistance...characterized by their ability to integrate their genomes into hosts in order to continue their lifecycle...gamma-retroviruses fall under the Retroviridae family. The genome of retroviruses is made of RNA. This retroviral...transcribed into DNA before being integrated in the genome of a host. Going from RNA to DNA is the reverse... the use of “retro”. Retroviruses have simpler genomes, containing only the necessary packaging genes,...lentiviral vector guide . Gamma-Retroviral Vectors The genome of gamma-retroviruses ranges from 9–11 kb, encoded...provirus, the cDNA that will be integrated into a host genome. This single strand of RNA contains packaging genes...

  4. Plan Your Experiment

    Type
    Guide
    ...sequence and your genomic target may reduce the gRNA activity. Be sure to sequence the genomic region you intend...system that enables researchers to manipulate the genome with relative ease. Over the past decade, researchers...Homology Directed Repair (HDR) Generate specific genomic edits, like point mutations or small tag insertions...gene expression without permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional...gene expression without permanently modifying the genome sequence dCas9 fusion with transcriptional activator... chosen Cas enzyme at the right spot within the genome relies on proper gRNA design. While there are some...sequence with no homologous sites elsewhere in the genome. In reality, a given gRNA target sequence will ...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Random integration of rAAV genomes in non-homologous sites of the host genome can occur, although at a ...containing a small, linear single-stranded DNA (ssDNA) genome. Non-enveloped viruses do not have a lipid bilayer... and the viral genetic material inside. The AAV genome is ~4.7 kb long and consists of two open reading...Rep68, Rep52, and Rep40), which are involved in genome replication and viral packaging. The Cap region... signals for packaging. Figure 1: Wild-type AAV genome. Created with BioRender.com. AAVs are part of the...medium-sized, and contain a double-stranded DNA genome. Adeno-associated viruses (AAV), however, are small...correlates to the size of the DNA removed from the viral genome (~4.7 kb). For rAAV vector production, the Rep ...

  6. Adenovirus Guide

    Type
    Guide
    ...the left arm of the genome adjacent to the left ITR that is required for viral genome packaging. In helper...containing a linear double-stranded DNA (dsDNA) genome. Non-enveloped viruses do not have a lipid bilayer... the viral genetic material inside. Adenoviral genomes range between 26–45 kb in length and contain multiple...virions. Figure 1: Wild-type adenovirus and its genome. Genes over or under the line are in forward or...medium-sized, and contain a double-stranded DNA genome. Adeno-associated viruses (AAV), however, are small...correlates to the size of the DNA removed from the viral genome. Over time, subsequent generations of rAdV vectors...plasmid — containing the rest of the adenoviral genome necessary for viral production and homology regions...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...genes. Some libraries cover the majority of the genome, while others are restricted to certain gene sets.... Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our Viral...Knockout Screening Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens...

  8. Molecular Biology Reference

    Type
    Guide
    ... of short RNAs. Genome Engineering Plasmids - Used to target and edit genomes. Genome editing is most ...thousand basepairs from the entire human genome. Digesting the human genome with restriction enzymes would yield...plasmids upon cell division in addition to their own genomic DNA. Because of this additional replication burden...guide RNAs that target specific locations in the genome. For more information on CRISPR check out Addgene...Viral Plasmids - These plasmids are modified viral genomes that are used to efficiently deliver genetic material... to sequence an entire plasmid or an organism’s genome. One Sanger sequencing reaction would give you ...

  9. Science Guides

    Type
    Guide
    ...immune system in bacteria, have been modified for genome engineering. Find essential background information...genes. Some libraries cover the majority of the genome, while others are restricted to certain gene sets...

  10. Educational Resources

    Type
    Guide
    ...related molecular biology techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors...

  11. Addgene's eBook Collection

    Type
    Guide
    ...designing your gRNA and how to use pooled libraries in genome-wide screens. Close About Overlay Select Fluorescent...

  12. Cloning

    Type
    Guide
    ... like... Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO...
Showing: 1 - 12 of 12 results