We narrowed to 81 results for: genome

...systematically perturbs the genome to assess the impact of genomic variation on genome function and phenotype...Consortium investigators to examine how genomic variation affects genome function to influence phenotypes. ...new window) aims to understand how genomic variation affects genome function, which in turn impacts phenotype...and map how genome function shapes phenotype, and how these processes are affected by genomic variation....will produce a catalog of the impact of genomic variants on genome function and phenotypes. Plasmids The...Depositor Collections IGVF Consortium Impact of Genomic Variation on Function (IGVF) Consortium Collection...window) (Link opens in a new window) The Impact of Genomic Variation on Function (IGVF) Consortium (Link opens...

...that cleaves the genomic DNA. Type II CRISPR was the first system harnessed for genome engineering, with...anti-CRISPRs as off-switches for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek...valuable genome engineering tool. CRISPR... CRISPR CRISPR History CRISPR History and Development for Genome Engineering...prokaryotic immune system is especially amenable to genome engineering, offering flexibility and easy multiplexing...more so) for research applications, eclipsing past genome engineering technologies like zinc finger nucleases...sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard against...

...Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing...plasmids related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems...for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning and Genomic Tools Synthetic...Synthetic Biology: Cloning and Genomic Tools SynBio Resources SynBio Home Browse All SynBio Assembly Standards... systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration...Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by the...

...1000000084 pDGE Dicot Genome Editing Kit Plasmids to assemble constructs for genome editing in dicotyledonous... for cloning, gene expression, reporter assays, genome editing, gene silencing, and more. Plasmid...Plasmids Plant Plasmids and Resources Cloning & SynBio Genome Editing Gene Silencing Reporters Addgene’s repository...variety of molecular biology technologies: from genome engineering and gene expression to reporter systems...1000000236 MoClo Plant Parts III: Transformation & Genome Engineering Kit Collection of MoClo Level 0 parts...(#1000000036). Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible expression, and...in transgenic analysis of plants. Return to top Genome Editing The following table contains a curated ...

...programmable for different genomic loci, capable of detecting multiple genomic loci, and compatible with...non-repetitive genomic loci, as well as chromosome painting in living cells. Visualizing a specific genomic locus... visualization of genomic loci. CRISPR... CRISPR Visualize CRISPR Plasmids: Visualize Genomic Loci Mammalian Resources...with fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages over...sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering multiple... chromosome. Figure 1: Overview of visualizing genomic loci using CRISPR. Created with BioRender.com. ...

...that defines the genomic target to be modified. You can therefore change the genomic target of the Cas...of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide...ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-...not permanently modify the genomic DNA. dCas9 has also been used for genome-wide screens to activate or...visualizing genomic loci with fluorophores using CRISPR. Browse Plasmids: Visualize Purify Genomic Regions...RNAs), and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...associated with a genomic region of interest in vivo. Figure 12: Overview of purifying genomic regions using...

...Vector Type Expression Genome Packaging Capacity Virus Size Cells Infected Host Genome Integration Immune ...expression and are permanently integrated into the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated...expression, but generally don't integrate into the host genome. AAVs are limited by their small packaging capacity...cells. They integrate permanently into the host genome, enabling long-term transgene expression. Retroviral...vectors also vary in the composition of their viral genome, expression of the transgene, packaging capacity...Recombinant AAV has a low frequency of target cell genome integration. Introduction to Viral Vectors Figure... goal, which is to make more viruses. The viral genome contains only the necessary genes to achieve this...

...associated with a genomic region of interest in vivo. Figure 1: Overview of purification of genomic loci using... CRISPR Purify CRISPR: Purify Genomic Loci Mammalian Bacteria Yeast Resources Identifying...Identifying molecules associated with a genomic region of interest in vivo is essential to understanding locus...immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular gRNA. In the...catalytically inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag or tags can...epitope tag. Compared to conventional methods for genomic purification, CRISPR-based purification methods...CRISPR plasmids designed for the purification of genomic loci. To learn more about purification using CRISPR...

...off-targets, target's genomic location, and genome annotation. Available genomes are human (hg19 & hg38...design tool for genome editing, as well as gene regulation (repression and activation). Genome support for...-use computational pipeline for the analysis of genome editing experiments. It enables rapid and intuitive... a new window) . MAGeCK Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can...selected sgRNAs, genes, or pathways. For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology...prediction. Developed by the Hyongbum Kim Lab . GenET (Genome Editing Toolkit) (Link opens in a new window) A...well as predict off-targets, for CRISPR in 180+ genomes. Developed by the Jean-Paul Concordet Lab . For...

...are below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to ...vectors for genome editing in the brain Using Cas9 mice Platt et al. demonstrated in vivo genome editing ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al ., Science...libraries (both human and mouse) for performing genome-wide knockout screens. The libraries are available...Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab has both single- and dual-vector...top AAV - Cas9 mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of...

...sequence and your genomic target may reduce the gRNA activity. Be sure to sequence the genomic region you intend...system that enables researchers to manipulate the genome with relative ease. Over the past decade, researchers...Homology Directed Repair (HDR) Generate specific genomic edits, like point mutations or small tag insertions...gene expression without permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional...gene expression without permanently modifying the genome sequence dCas9 fusion with transcriptional activator... chosen Cas enzyme at the right spot within the genome relies on proper gRNA design. While there are some...sequence with no homologous sites elsewhere in the genome. In reality, a given gRNA target sequence will ...

...Oxford Collection Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids - Oxford...Structural Genomics Consortium (SGC) Oxford collection Plasmid... Plasmid Collections Structural Genomics Consortium...

...Stockholm Collection Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids - Stockholm...Structural Genomics Consortium (SGC) Stockholm collection Plasmid... Plasmid Collections Structural Genomics Consortium...

...Toronto collection Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids - Toronto...Structural Genomics Consortium (SGC) Toronto collection Plasmid... Plasmid Collections Structural Genomics Consortium...

...fluorescence microscopy experiments. The C. elegans genome is well studied and hundreds of reporters have ... be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein Lab. Contains a set...from John Calarco's lab as described in Heritable genome editing in C. elegans via a CRISPR-Cas9 system....Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's lab ...described in A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans. A modified approach...and distributes stocks of C. elegans. Silencing Genomes - Cold Spring Harbor's guide to RNAi in C. elegans...accessible information concerning the genetics, genomics and biology of C. elegans and related nematodes...

...a given genomic locus. Visualize CRISPR fluorescence methods can be used to visualize genomic loci. gRNAs...bacterial immune system to a revolutionary tool for genome engineering. CRISPR Topic Page : Want the latest...experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas enzymes generate ...formation. Delete Cascade-Cas3 generates large genomic deletions. Insert CRISPR transposases insert large...Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused to an epitope...

...need before starting your experiment. Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs...all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled...Collection Cell Migration Consortium Impact of Genomic Variation on Function (IGVF) Consortium Krüppel-like... for Solute Carrier Proteins (SLCs) Structural Genomics Consortium Zinc Finger Consortium Pathways & ORFs...

...Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish... zebrafish research. This includes plasmids for genome editing, empty backbones, fluorescent proteins,... Zon Lab Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system - Shuo Lin ...lineage tracing by combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous single-...fluorescent proteins for tagging genes at endogenous genomic loci in zebrafish. NTR 2.0 - Jeff Mumm Lab. NTR...Database (ZFIN) - A curated database of genetic, genomic, and developmental data for zebrafish biology. .... Seurat - R package for analyzing single cell genomics in zebrafish. Return to top Do you have suggestions...

...Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1 promoter that...Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression of the tTA from the...Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV2 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...

...Collections Structural Genomics Consortium Plasmids Browse All SGC Plasmids Structural Genomics Consortium Plasmids...Browse plasmids from the Structural Genomics Consortium (SGC) for 3D protein structure studies Plasmid...