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TypeGuide...Figure 2): Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ...
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Adenovirus Guide
TypeGuide...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ...capacity is large enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle... -
Adeno-associated virus (AAV) Guide
TypeGuide...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ... Cas9 from Streptococcus pyogenes (SpCas9) and a gRNA together into an rAAV capsid is challenging due ... -
Addgene's eBook Collection
TypeGuide...planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide screens... -
Promoters
TypeGuide...also transcribes small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including our popular...