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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 297 results for: hal.2

Showing: 241 - 260 of 297 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first... Glucose Glucose, methanol, glycerol Heterothallic mating Efficient Efficient Inefficient...

  2. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...axon terminals to the soma (retrograde tracer) (Fig. 2). Viruses as neuronal scalpels Viruses can be injected...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...

  3. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...of science, communication isn't easy.  But the challenge is worthwhile and rewarding. A truly passionate...

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...retransduce a model organism such as mice, e.g. first at 2 months, then at 4 months? Or do antibodies become ...prohibitive problem? A: Re-administration is a challenge for AAV due to the development of neutralizing...

  5. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...account when simulating a digest as depicted in Lane 2. Controlling methylation Finally, you can control...plasmids. It turns out that restriction enzymes are one half of naturally occurring restriction modification ...

  6. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...PMID: 24901218. PubMed Central PMCID: PMC4047077. 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata ...Turning on the UV light 3-4 times a week for up to half an hour can help keep your hood contamination free...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

  7. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...PMID: 25538079. PubMed Central PMCID: PMC4353587. 2. Stephen, Andrew G., et al. "Dragging ras back in ...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

  8. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig...However, the cargo capacity of AAV is ~4.7 kb, almost half that of lenti and gamma-retro viruses. This is large...

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...hardware, this process is often much more involved. Challenges such as the provision of an accessible supply...

  10. Hot Plasmids - May 2022

    Type
    Blog Post
    ... those that also infect the liver.    Fig. 2: Summary of the generational evolution of the AAV ...Leeson Multiplexed images in live cells remains a challenge due to multiple factors, including limitations...

  11. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication ...encoded only once, and each <200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will...

  12. Summer SciComm: Preprints

    Type
    Blog Post
    ... scientific communication ecosystem Figure 2: The scientific ecosystem, enriched by open access...Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (2022) A guide to preprinting for...

  13. Plasmids 101: Cre-lox

    Type
    Blog Post
    ..., N. and Hamilton, D. 1981. PubMed PMID: 6276558. 2. Site-specific DNA recombination in mammalian cells...in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology,...

  14. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...to the break room for a sip of water.  Fig 2: Blugene likes to spark creativity in out on the open... needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding ... review it. This survey generated 26 ideas, over half of which, when implemented, impacted the entire ...you can, set aside time to prepare for future challenges. This doesn’t have to be time-consuming or even... keeping your science going throughout these challenging times.  Take a Break! If all else fails, one ...

  15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin..., Gautier A. Nat Commun. 2019 Arnaud Gautier HaloTag TagBiFC allows exogenous labeling for single-molecule...and reversible complementation. TagBiFC (split HaloTag) provides maximum flexibility in color labeling...

  16. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...this complex, developing Cas3 tools has been challenging. It can be difficult to ensure comparable expression..., though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have one...

  18. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...that view (Callahan et al., 2022).     Fig. 2: Students in an MDH CURE replaced select residues ... Biology (Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their...experience on their resumes). CUREs are not without challenges. Real research is messy, and it can be difficult...

  19. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves a substrate, which will then...transgenic hairy root lines in tissue cultures takes half a year, and generating a transgenic plant can take...Luciferases usually have shorter mRNA and protein half-lives then GUS and are therefore good temporal reporters...

  20. Bioinformatics at Addgene

    Type
    Blog Post
    ...the necessary steps to perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline...300,000 individual nucleotide sequences. More than half of these sequences were generated by Addgene as ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to...
Showing: 241 - 260 of 297 results