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We narrowed to 266 results for: his-tag

Showing: 241 - 260 of 266 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ... Tabebordbar et al. Long et al. show that the percentage of dystrophin-expressing muscle cells increases... blot, at <10% of the wild-type level. Muscle histology is improved, with vastly reduced inflammation ...delivery must be optimized to: 1. Reach a high percentage of muscle cells throughout the body, especially...

  2. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to...fluorophores to visualize multiple targets at once. An advantage of multiplexing is that you can ask how multiple...choose can make or break your experiment, so take advantage of all the available resources and give yourself...

  3. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... of the gel. For small proteins, use a higher percentage of acrylamide to increase migration resistance...improve separation. For large proteins, reduce the percentage of acrylamide. To further improve resolution,...and references References Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory...

  4. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-elite journals... recently reported that the most cited work in history is a 1951 description of an assay to determine ...

  5. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...modified from Ke et al. 2017. 1. Among scientists, historians have the most Twitter users. Biologists rank .... The #1 (re)tweeted domain by scientists was Instagram Social media and news sites were also popular....

  6. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...Additionally, the presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...

  7. Science Career Options

    Type
    Blog Post
    ...technology, and good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel,...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities...

  8. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...CRISPR tools, without compromising their strategic advantages in gene editing, while keeping the system to ...multiplex genome engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...have enhanced the ability of labs to answer sophisticated questions about cellular processes. Currently... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical...who are interested in such careers should take advantage of their academic environment and attend seminars...

  11. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ... PAM requirement, and how these proteins are advantageous in today’s genome editing landscape. The need...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

  12. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...isotypes and subclasses. They each have their own advantages. Since monoclonal antibodies contain a single...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector for ...Naldini,L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6...

  15. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...expansion.  In some cases, labels are added after this stage.  Step 4: Expand! (Typically by just adding water... L (2022) Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...that retain the ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct...PMID: 7768854. PubMed Central PMCID: PMC177048. Thisted, Thomas, and Kenn Gerdes. "Mechanism of post-segregational...

  17. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...post-injection. Livers from these mice were histologically normal and liver injury markers were not increased...also be packaged into AAV. NmeCas9 has the added advantage that it can be turned off by anti-CRISPR proteins...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides... a different PAM site and generates 5’ and 3’ staggered ends after dsDNA breaks. The Doudna lab discovered...

  19. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based...cover letters for entry-level positions, as at that stage, cover letters can be a barrier for qualified applicants...

  20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-containing viral capsids is typically...Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization method (DNA...
Showing: 241 - 260 of 266 results