Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 568 results for: mme

Showing: 241 - 260 of 568 results

  2. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... liquid containers closed when they aren’t in immediate use. This will prevent spills, but, if any spills...occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...believe that industry postdocs, having already been immersed in an industry environment, have a better understanding...or you want to end up in an industry job, being immersed in that environment sooner rather than later can...

  4. PhD Applications After COVID

    Type
    Blog Post
    ...during the application process. Many institutions recommend that you reach out to potential advisors before...professors per program. Most applications will recommend that you name two professors in your application...

  5. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...poured onto the middle of the plate, where it immediately spreads out to form a firm monolayer. The cell...the level of endogenous expression. We highly recommend the use of an electronic pipettor with variable...

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...to purchase the Cas9 protein and a gRNA from a commercial vendor, this can often be expensive. An alternative...transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components are...

  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... tag for protein purification and a number of commercial antibodies to GFP are available. Others: We’ve...interactions.   Do you have a favorite way to use GFP?  Comment and tell us how! And come back next week when we'll...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... variable and rabbit constant domains and is recommended for use in western blots (Figure 1). If you prefer...worm-optimized wrmScarlet. mScarlet-I3, while initially dimmer, showed greater photostability and also matured...

  17. Bioinformatics at Addgene

    Type
    Blog Post
    ...Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

  18. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...unprecedented specificity: when purified, it could be programmed to generate specific cuts on the RNA target. ...non-dividing and primary cells, and can be easily programmed for new genes. In comparison with other large...

  19. Hot Plasmids - November 2023

    Type
    Blog Post
    ...under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on the viral surface...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ... from Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...thereby increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of ...
Showing: 241 - 260 of 568 results