We narrowed to 565 results for: mme

1. Transferable Skills: Negotiation

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   Blog Post

   ...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...separately in a future post.   Resources Maria recommends: Getting to Yes: Negotiating Agreement Without...

2. Academic vs. Industry Postdocs

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   ...believe that industry postdocs, having already been immersed in an industry environment, have a better understanding...or you want to end up in an industry job, being immersed in that environment sooner rather than later can...

3. Cloning Mammalian Cells with the Agarose Method

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   ...poured onto the middle of the plate, where it immediately spreads out to form a firm monolayer. The cell...the level of endogenous expression. We highly recommend the use of an electronic pipettor with variable...

4. Google Forums Round Up: First Impressions of NgAgo

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   ...Addgene Blog - Leave suggestions and tips in the comments section below or contact [email protected] if you'd...scientific community on NgAgo forums, blogs, or in the comments section below. Keep pipetting and happy genome...

5. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...likely capabilities of RNA-guided gene drives in the summer of 2014 (after extensive discussions with biosecurity...sake of laboratory convenience. Disclaimer: Recommendations concerning the appropriate degree of confinement...

6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ...to purchase the Cas9 protein and a gRNA from a commercial vendor, this can often be expensive. An alternative...transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components are...

7. Droplet Digital PCR for AAV Quantitation

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   ...than 50 cycles is recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even...

8. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...to this established dye structure have yielded commercial panels of advanced rhodamine fluorophores, such...Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke D. Lavis...

9. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   ...a time.   Not everything that is received is immediately transformed and stored. Some samples need to ...to organize samples within your lab, we highly recommend using barcodes! Barcoded tubes are easily accessible...

10. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ... tag for protein purification and a number of commercial antibodies to GFP are available. Others: We’ve...interactions.   Do you have a favorite way to use GFP?  Comment and tell us how! And come back next week when we'll...

11. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    ...20-nt guide region binds to the DNA sequence immediately downstream of the TAM. This RNA-DNA hybrid formation...in your target gene. Choose a 20-nt sequence immediately downstream of the TAM for targeting. While plant-specific...

12. Hot Plasmids: Fall 2024

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    ... variable and rabbit constant domains and is recommended for use in western blots (Figure 1). If you prefer...worm-optimized wrmScarlet. mScarlet-I3, while initially dimmer, showed greater photostability and also matured...

13. Plasmids 101: Codon usage bias

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    ... from Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...thereby increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of ...

14. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ... translation requires the start codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized....org/10.1038/mtna.2016.48 Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) ...

15. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...rest of the genome.  2) The target is present immediately upstream of a Protospacer Adjacent Motif (PAM...where the edit is desired, and it performs the programmed edit within the editing window (bases 4-8 of ...

16. Bioinformatics at Addgene

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    ...Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

17. New CRISPR Tools: Cas7-11 and PASTE

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    ...unprecedented specificity: when purified, it could be programmed to generate specific cuts on the RNA target. ...non-dividing and primary cells, and can be easily programmed for new genes. In comparison with other large...

18. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    ...pair palindromic repeats separated by an 8 bp asymmetric core. Recombination between these sites can lead...discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp length that is ...

19. Supporting Reproducibility with a Connected ELN

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    ...librarians and data curators, IT managers, the commercialization office, the CIO’s office, and research computing...of the vibrant life sciences research and commercialization community in greater Boston, while at the ...

20. Hot Plasmids - November 2023

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    ...under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on the viral surface...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their...