We narrowed to 560 results for: mme
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TypeBlog Post...believe that industry postdocs, having already been immersed in an industry environment, have a better understanding...or you want to end up in an industry job, being immersed in that environment sooner rather than later can...
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Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...Addgene Blog - Leave suggestions and tips in the comments section below or contact [email protected] if you'd...scientific community on NgAgo forums, blogs, or in the comments section below. Keep pipetting and happy genome... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...likely capabilities of RNA-guided gene drives in the summer of 2014 (after extensive discussions with biosecurity...sake of laboratory convenience. Disclaimer: Recommendations concerning the appropriate degree of confinement... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...to purchase the Cas9 protein and a gRNA from a commercial vendor, this can often be expensive. An alternative...transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components are... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...than 50 cycles is recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...to this established dye structure have yielded commercial panels of advanced rhodamine fluorophores, such...Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke D. Lavis... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...a time. Not everything that is received is immediately transformed and stored. Some samples need to ...to organize samples within your lab, we highly recommend using barcodes! Barcoded tubes are easily accessible... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post... tag for protein purification and a number of commercial antibodies to GFP are available. Others: We’ve...interactions. Do you have a favorite way to use GFP? Comment and tell us how! And come back next week when we'll... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...bevacizumab has recently been added to the treatment recommendations for non-resectable mesothelioma by the National.... Senate. If this bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...librarians and data curators, IT managers, the commercialization office, the CIO’s office, and research computing...of the vibrant life sciences research and commercialization community in greater Boston, while at the ... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post... translation requires the start codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized....org/10.1038/mtna.2016.48 Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) ... -
Bioinformatics at Addgene
TypeBlog Post...Numpy, and Biopython. We started at the usual recommended place: documentation. We oriented ourselves with...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...unprecedented specificity: when purified, it could be programmed to generate specific cuts on the RNA target. ...non-dividing and primary cells, and can be easily programmed for new genes. In comparison with other large... -
Hot Plasmids - November 2023
TypeBlog Post...under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on the viral surface...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their... -
Hot Plasmids: Fall 2024
TypeBlog Post... variable and rabbit constant domains and is recommended for use in western blots (Figure 1). If you prefer...worm-optimized wrmScarlet. mScarlet-I3, while initially dimmer, showed greater photostability and also matured... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...rest of the genome. 2) The target is present immediately upstream of a Protospacer Adjacent Motif (PAM...where the edit is desired, and it performs the programmed edit within the editing window (bases 4-8 of ... -
Plasmids 101: Codon usage bias
TypeBlog Post... from Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...thereby increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of ... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...pair palindromic repeats separated by an 8 bp asymmetric core. Recombination between these sites can lead...discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp length that is ... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...we hear the word ‘antibiotic’, very often we immediately imagine a prescription for a bacterial infection...drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & HM Government (2016... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...the study, or even review Facebook and Twitter comments. On We Share science, video usurps the written...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...