We narrowed to 565 results for: mme

... Highly expressed markers should be paired with dimmer dyes so they don’t “wash out” the rest of the panel...anything (negative population). It is strongly recommended that when you first run a new panel on a spectral...

...during the application process. Many institutions recommend that you reach out to potential advisors before...professors per program. Most applications will recommend that you name two professors in your application...

...Addgene Blog - Leave suggestions and tips in the comments section below or contact [email protected] if you'd...scientific community on NgAgo forums, blogs, or in the comments section below. Keep pipetting and happy genome...

...likely capabilities of RNA-guided gene drives in the summer of 2014 (after extensive discussions with biosecurity...sake of laboratory convenience. Disclaimer: Recommendations concerning the appropriate degree of confinement...

...to purchase the Cas9 protein and a gRNA from a commercial vendor, this can often be expensive. An alternative...transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components are...

...than 50 cycles is recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even...

...to this established dye structure have yielded commercial panels of advanced rhodamine fluorophores, such...Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke D. Lavis...

...a time.   Not everything that is received is immediately transformed and stored. Some samples need to ...to organize samples within your lab, we highly recommend using barcodes! Barcoded tubes are easily accessible...

... tag for protein purification and a number of commercial antibodies to GFP are available. Others: We’ve...interactions.   Do you have a favorite way to use GFP?  Comment and tell us how! And come back next week when we'll...

...pair palindromic repeats separated by an 8 bp asymmetric core. Recombination between these sites can lead...discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp length that is ...

...bevacizumab has recently been added to the treatment recommendations for non-resectable mesothelioma by the National.... Senate. If this bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that...

... from Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...thereby increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of ...

... translation requires the start codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized....org/10.1038/mtna.2016.48 Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) ...

...rest of the genome.  2) The target is present immediately upstream of a Protospacer Adjacent Motif (PAM...where the edit is desired, and it performs the programmed edit within the editing window (bases 4-8 of ...

...Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

...unprecedented specificity: when purified, it could be programmed to generate specific cuts on the RNA target. ...non-dividing and primary cells, and can be easily programmed for new genes. In comparison with other large...

...librarians and data curators, IT managers, the commercialization office, the CIO’s office, and research computing...of the vibrant life sciences research and commercialization community in greater Boston, while at the ...

...under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on the viral surface...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their...

...20-nt guide region binds to the DNA sequence immediately downstream of the TAM. This RNA-DNA hybrid formation...in your target gene. Choose a 20-nt sequence immediately downstream of the TAM for targeting. While plant-specific...

... variable and rabbit constant domains and is recommended for use in western blots (Figure 1). If you prefer...worm-optimized wrmScarlet. mScarlet-I3, while initially dimmer, showed greater photostability and also matured...