We narrowed to 577 results for: multi

...individual genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the... then packaged into AAV for in vivo delivery. Multiplex gene targeting is also possible and is only constrained...vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator...

...polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

... not, multiple techniques are used as part of a treatment plan, an approach known as multimodal therapy...are some genetic factors involved. For example, multiple studies show that certain mutations of the BAP1...

...et al., 2019). Because of its ability to target multiple gene loci simultaneously, CRISPRa can also be ...(Chavez et al., 2015)) are good choices across multiple cell lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3...Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods 13:563–567 . https://doi....

...chromosomal location, sequence context, and cell type. Multiple labs worked to improve its effectiveness through... a machine learning approach, the lab proposed multiple sets of Cas9 mutations that conferred new PAM ...Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science...

...calculate things about earlier ones. In practice, multiple sources of error (red) influence these relationships...not perturbed by the experiment Unfortunately, multiple reports suggest that common housekeeping proteins... to worry about the linear detection ranges of multiple targets. Convenient! Comparison of loading control...

...with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although this ...for lactate developed using a high-throughput multiparameter screen (Koveal et al. 2022). Lactate is a key... here!   Koveal D, et al. A high-throughput multiparameter screen for accelerated development and optimization...

... added to the reaction for easy detection. The multiple biotin binding sites found on avidin are also ...during PCR using a biotin labelled dUTP. There are multiple types of biotin labels that can be added to an...Biotin labelled oligonucleotides can be used in a multitude of applications including but not limited to in...

.... Digestion with enzyme “B” detects primer multimerization (purple) which is evident by an increase in...CRISPR-induced genome modifications. Occasionally, multimerization of the PCR primers can cause duplication of...particularly relevant in cases where a large plasmid with multiple functional regions is used as template for the...

...assembly is well known for allowing easy assembly of multiple linear DNA fragments, but can also be used in ...biology, mainly because of the ease in assembling multiple fragments in one step with no scar sequences remaining...Whether it is for routine cloning, assembly of multiple fragments, or synthetic biology, you should consider...

...is necessary or desirable to control or look at multiple genes at the same time.  The 3 binary systems ...analysis in Drosophila or for precise control over multiple genes in synthetic systems. Recent work has even... Multiple Authors...

...about 100 bps into the run, it got “dirty”, with multiple sequence traces overlapping each other! When the... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering...

...microscope. You may be wondering why there are multiple objective lenses. Each objective performs essentially...determine the final magnification of your image, multiply the magnification of your objective lens by the...

...counts making them fast and viable options for multiplex sequencing in many laboratories. Nevertheless,...DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic...dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to...

...location in your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere ...Annealed-oligo Cloning . If you are lucky enough to have multiple options for enzymes that flank your insert and...

... protein because the antibodies will recognize multiple epitopes on the protein. However, each animal ...advantage of flow cytometry is the ability to use multiple fluorescent signals (up to a dozen or more!) to...relative or absolute amount of the expression of multiple proteins on the same cell simultaneously as well...

...Chloride Close the bottle and mix by inverting multiple times Adjust pH to ~8.5 Filter sterilize through...DMEM Complete. Pipet back and forth vigorously multiple times to obtain a single cell suspension (no clumps...

...way to increase CRISPR editing efficiency across multiple CRISPR enzymes. Plasmids for CRISPR coselection...segment on regulating telomere length with TZAP Multiplexible plant genome engineering toolkit The Voytas ... well as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components...

...KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv ...Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers...

... they have developed this further to provide multiplexed detection, higher sensitivity, and more. Read...lab compared the efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases...