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We narrowed to 575 results for: multi

Showing: 241 - 260 of 575 results

  1. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...individual genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the... then packaged into AAV for in vivo delivery. Multiplex gene targeting is also possible and is only constrained...vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

  3. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... not, multiple techniques are used as part of a treatment plan, an approach known as multimodal therapy...are some genetic factors involved. For example, multiple studies show that certain mutations of the BAP1...

  4. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate AAV vectors, increasing overall... AHM, Zhu K, Wagers AJ, Church GM (2016) A multifunctional AAV–CRISPR–Cas9 and its host response. Nat ... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...

  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...CRBNmidi alone (A) and in a ternary complex with the multiple myeloma drug mezigdomide and ubiquitination substrate...mutations.  Here, the researchers were able to target multiple genes in M. abscessus either simultaneously or... Multiple Authors...

  6. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...maintain itself within host cells.  The plasmid has multiple ori that are functionally differentiated from ...proteins from bacterial hosts. In a plasmid with multiple oris, one may function in one type of host and...only one ori and a broad host range plasmid with multiple oris that vary in DnaA-box and iteron structure...

  7. Hot Plasmids - November 2023

    Type
    Blog Post
    ...different viral families and can be used to target multiple delivery systems, such as lentivirus and MMLV ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi... Multiple Authors...

  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...et al., 2019). Because of its ability to target multiple gene loci simultaneously, CRISPRa can also be ...(Chavez et al., 2015)) are good choices across multiple cell lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3...Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods 13:563–567 . https://doi....

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... added to the reaction for easy detection. The multiple biotin binding sites found on avidin are also ...during PCR using a biotin labelled dUTP. There are multiple types of biotin labels that can be added to an...Biotin labelled oligonucleotides can be used in a multitude of applications including but not limited to in...

  10. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...calculate things about earlier ones. In practice, multiple sources of error (red) influence these relationships...not perturbed by the experiment Unfortunately, multiple reports suggest that common housekeeping proteins... to worry about the linear detection ranges of multiple targets. Convenient! Comparison of loading control...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although this ...for lactate developed using a high-throughput multiparameter screen (Koveal et al. 2022). Lactate is a key... here!   Koveal D, et al. A high-throughput multiparameter screen for accelerated development and optimization...

  12. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    .... Digestion with enzyme “B” detects primer multimerization (purple) which is evident by an increase in...CRISPR-induced genome modifications. Occasionally, multimerization of the PCR primers can cause duplication of...particularly relevant in cases where a large plasmid with multiple functional regions is used as template for the...

  13. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...assembly is well known for allowing easy assembly of multiple linear DNA fragments, but can also be used in ...biology, mainly because of the ease in assembling multiple fragments in one step with no scar sequences remaining...Whether it is for routine cloning, assembly of multiple fragments, or synthetic biology, you should consider...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...is necessary or desirable to control or look at multiple genes at the same time.  The 3 binary systems ...analysis in Drosophila or for precise control over multiple genes in synthetic systems. Recent work has even... Multiple Authors...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...about 100 bps into the run, it got “dirty”, with multiple sequence traces overlapping each other! When the... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering...

  16. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...counts making them fast and viable options for multiplex sequencing in many laboratories. Nevertheless,...DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic...dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to...

  17. Using a Light Microscope Protocol

    Type
    Protocol
    ...microscope. You may be wondering why there are multiple objective lenses. Each objective performs essentially...determine the final magnification of your image, multiply the magnification of your objective lens by the...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... protein because the antibodies will recognize multiple epitopes on the protein. However, each animal ...advantage of flow cytometry is the ability to use multiple fluorescent signals (up to a dozen or more!) to...relative or absolute amount of the expression of multiple proteins on the same cell simultaneously as well...

  19. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...location in your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere ...Annealed-oligo Cloning . If you are lucky enough to have multiple options for enzymes that flank your insert and...

  20. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Chloride Close the bottle and mix by inverting multiple times Adjust pH to ~8.5 Filter sterilize through...DMEM Complete. Pipet back and forth vigorously multiple times to obtain a single cell suspension (no clumps...
Showing: 241 - 260 of 575 results