...scientific community represents the culmination of many such experiences. For example, students in Provost’s ...sciences by making a greater number of students active participants in the research process – so if this...provide the same kinds of opportunities for his students. But he also found that his teaching responsibilities...decided to adopt a more focused format, where all students work on malate dehydrogenase (MDH), a citric acid...ultimately settled on a two-semester model, in which students learn to express, purify, and analyze MDH in semester...skills to design and carry out their own novel experiments in semester two (Knutson et al., 2010a,b).    ...research activities in a CURE must be authentic – students should be attempting to generate new knowledge...

...recombinases, regulatory elements, developmental regulators, and lots of viral components. This kit is also ...bacteria, protists, plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters...systems, mammalian cells, yeast, flies, and even plants! In this blog we will review common uses of MoClo...chemical synthesis of these molecules. To make large amounts of pure product in an affordable way, researchers...Pérez-González et al., 2017). In addition to additional elements for S. cerevisiae chromosomal integration and ...great starter for your general plant cloning experiments. Addgene also has two additional MoClo plant ... a variety of Cas enzymes with different PAM requirements and editing abilities, such as base editing,...

...are too few resources to teach students how to perform these experiments in the beginning. In most classrooms...lab publication [1] so that the students performing these experiments have a good baseline for comparison...techniques such as basic observation and dissections. Students don’t usually perform more modern techniques used...in genetics or molecular biology because the experiments are either not accessible or too challenging ...easier to perform advanced developmental biology experiments in planarians with their recent plasmid deposit...deposit. These plasmids enable two main types of experiments: Whole mount in Situ Hybridization (WISH) for... expression. The 49 deposited plasmids enable students to study gene expression and functions of key ...

... sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping...between two non-fluorescent split GFP fragments. When the two fragments, expressed in the presynaptic region...genome. They did this by selecting the important elements from existing transposon and vector systems and...an all-synthetic vector that included only the elements needed for function. The lab validated this vector..., particular for those involving recombination elements such as FRT or Lox sites, and provides a modular...system for easier exchange and re-use of existing elements. DNA sequences for a desired component are cloned... Gate cloning step to assemble the individual components into a destination vector in a predefined order...

... reliable reagents for future research. I’ve talked to many researchers who have complaints about the ...provide you with additional validated reagents, and, with these reagents in hand you’ve got a good foundation...tries 5 separate experiments at once to get confirmatory data for a hypothesis and presents only the results... Scientific reproducibility As Jeffrey Flier points out in his talk (1:24 - 29:31), there are a variety...Freedman et al 2015). Finally, as Pamela Hines of AAAS points out on the panel, irreproducibility undermines ... 1: Inability to repeat previously performed experiments Researchers should be able to follow one another...another’s protocols and use one another’s reagents with the confidence that they aren’t simply doing an ...

...60.2%) 20 graduate students (25.6%) 12 masters students (15.4%) 46 (59%) of these students were in the last...-program self-assessment, participants completed follow-up self assessments and program evaluations every...self-assesment. Achievements directly attributable to participation in the program included improvements in writing... got a physical award, all the students experienced great improvements, not necessarily quantifiable, ...mentorship during undergraduate and graduate studies. Students also need more access to reputable, organized ... networks connecting international experts and students. In response to such needs, the International ... BWH and providing career mentoring to several students from Spain and all over the world, she thought...

...particular, two recent events have the potential to influence both graduate students and postdocs broadly... Board to allow graduate students who work as teaching or research assistants to join unions. The topic...example, Yale graduate students filed for union elections in only 10 departments across the university....flexibility to address the unique needs of students from different departments across the university and alterations...advocacy skills “bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org...F1000Research, 2014; Dolan, F100Research, 2016). These events have also started progress towards solutions. The...which several projects were initiated. Recent developments promise to further change the landscape facing...

...minimal upfront requirements and allows you to expediently join multiple synthetic fragments, create multiple...refers to a method of physically joining multiple fragments of DNA to create a synthetically designed DNA ...multiple mutations in one or multiple fragments, and generate constructs for producing single-guide RNAs... yielding scarless DNA assembly, and allowing fragments to be taken directly from restriction enzyme digests...the absolute end of a fragment. This means that fragments (containing regions of homology) cut from plasmids...planning. This cloning methodology requires that the fragments to be joined contain regions of homology at, or...homology can be contained within the individual fragments themselves, but doesn’t necessarily have to be...

...works in the lab knows how expensive tiny amounts of reagents are, how complicated (and again, expensive...FD-CF pellets are easy for students to use in the classroom because the students simply need to add water...Most importantly, experiments for FD-CF reactions can be fun and engaging for students. In our BioBits ...example, we’ve designed FD-CF experiments that result in outputs that students can easily sense, whether ...consider using some elements of BioBits to transition your undergraduate students from the classroom to...had this kind of experience earlier! After all, students build circuits in physics class to learn about...funding and the resources to provide them to their students. My desire to create a set of low-cost, easy-to-use...

...well treatments are working to fight the cancer.  Beyond early modifications to standard treatments and...are running out of hope with more traditional treatments, such as surgery, radiation, and chemotherapy...sufferers can see great promise in up and coming treatments like immunotherapy. Mesothelioma Mesothelioma...and late discovery mean that most mesothelioma patients have an extremely poor prognosis. Together, the...disease with only about 3,000 new mesothelioma patients diagnosed every year in the US. Therefore, mesothelioma...many forms of cancer, the primary conventional treatments for mesothelioma include surgery to remove cancerous...cases the cancer is diagnosed so late that these treatments do not work very well. The best option in such...

...failure to clearly articulate one's talents and ideas. Some key points from my ebook, "Applying for a Faculty...success is publications and grants. 2. Write succinct and easy to understand documents A common misconception...example documents. This part has generated a lot of positive feedback. Many of these documents are too...sections, along with new thoughts on research statements. Snapp, who is always looking for ways to improve...improve his eBook, adds: "If you have any questions, comments or suggestions, please do not hesitate to email... members will NOT be in your area. Therefore, documents need to minimize jargon. Equally importantly, ...seminar See item 2. Attendees will include grad students (that are solicited for feedback), possibly the...

...department worked with students to maintain these time goals during COVID-19? How are students funded? Are there... outside of just the program requirements: by talking to current students about the work environment and...and standards for hybrid, virtual, and in-person events change seemingly daily (and are inconsistent between... you’re applying to a school that requires its students to do rotations in different labs before selecting... universities offer free practice sessions for students doing virtual interviews, so check if yours does...keep yourself organized. By writing down your key points and keeping them at-hand, you can quickly reference...universities typically invite a select group of students to continue the application process through a ...

...compatible with all your components. Next, consider the best form for those CRISPR components based on your model...introducing those CRISPR components into your target cells. Delivery of these components into mammalian cell...considered during the planning process. For CRISPR experiments, the following framework can help get you started...most effectively achieved with different CRISPR components. Select the appropriate CRISPR tools for your...will you be editing? Do you want to deliver the components as DNA, or would mRNA or protein delivery be ...of the first steps is to identify what CRISPR components you will need to deliver. Minimally, one or more...model system. CRISPR reagents can be delivered via transfection, nucleofection, viral transduction or ...

...green (v857) variants differing in kinetics, affinity, and ∆F/F0. Introduce the two variants in three different...The new iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and...models. Furthermore, they created three affinity variants – A184S (increased affinity for glutamate), S72A...iGluSnFR had limitations: they were not optimal for experiments requiring high signal to noise ratios, imaging...imaging of high frequency glutamate signaling events, and accurately differentiating between synaptic vs. ...Kaspar Podgorski’s lab have recently made iGluSnFR3 variants that are more sensitive and better localized for...predecessors. The detailed characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve...

...split into two fragments that individually lack fluorescence. When complementary FP fragments are fused to...construction Fusing FP fragments to your PIPs requires careful selection of FP fragments and, preferably, some...that underlie protein function. However, FRET experiments can be difficult to implement in practice because...hypothesized protein interaction partners (PIPs), the FP fragments form the complete FP because the PIPs they’re ...small-molecule inhibitors of a particular PPI. In such experiments, small-molecule inhibitors are tested in parallel...Design a BiFC Experiment Much like FRET, BiFC experiments must be carefully optimized to be successful....will allow you to determine where to fuse the FP fragments. In the absence of this information, you can fuse...

...more and more students are asking. Teachers are also asking, “How do I engage my students in real-world...the study of synthetic biology into the hands of students and teachers in the U.S. and beyond. “I want to...programs all focused on current questions and experiments in the field of synthetic biology. Founded as...excitement found through experiential learning. “Students may forget the technical details they hear in ...their classes,” she said, “but they remember the moments when they discover something new for themselves...asked in MIT’s research labs, BioBuilder inspires students and teachers to apply engineering principles to... biology and transforms them into modules that students and teachers can investigate together. Teachers...

... This means they do not reliably detect editing events that occur in less that 5% of the population (Fu...standardized thresholds for validating CRISPR experiments, many are turning to next-generation sequencing...genome predicted to contain off-target cleavage events. Unbiased techniques search the whole genome for...Frequencies of off-target mutations are essential data points for investigators looking to definitively link ...irreproducible data in a research setting, but these events could have disastrous health effects in the clinic...current algorithms do not take into account other elements, including those relating to DNA structure (e....and HTGTS (Frock et al. 2015) for cell-based experiments. These strategies can be used in concert with...

...threshold requirements: (1) that you have international recognition for your outstanding achievements in your...whenever you have new accomplishments to add. Obtain copies and translations of documents that are not in English... candidate meets the requirements set forth by USCIS before engaging with clients. To learn more, visit..., Esq. Founder of Casseus Law. Disclaimer: The contents of this post are intended to convey general information...and not to provide legal advice or opinions. The contents of this post should not be relied upon for legal...presented may not reflect the most current legal developments.  Further, this post may contain technical inaccuracies...actions taken or not taken based on any or all the contents of this post.  An attorney should be contacted...

...CRISPR experiments that involve HDR, electroporation can be combined with cell-type specific reagents in ...RNP-mediated CRISPR experiments While using Cas9-gRNA RNPs for CRISPR experiments can reduce many potential...result, RNP mediated CRISPR experiments may not be suitable for experiments where stable Cas9 expression...experiment requires delivery of, at minimum, two components: the Cas9 protein and a guide RNA (gRNA) targeting...are many advantages to using RNPs for CRISPR experiments. The RNP method can often be used in cells that...generation or homologous recombination.  However, experiments that require long-term expression of Cas9, such... by commercial vendors such as IDT. These two components are then incubated together to form the RNP. ...

...Kim et al., 2017 SaBE3, BE3 PAM variants, BE3 editing window variants   Greatly expands the number of ...editing work? Base transition editing requires three elements. They are, broadly: A Cas protein (catalytically...editing window specified by the Cas protein These elements were the starting point towards the development...inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change the U...development of BE3, many research groups have made improvements to base editors, including: expanding targeting...upstream of the PAM.  David Liu’s lab generated BE3 variants with other deaminases: AID, CDA1, and APOBEC3G... Liu lab also used natural and engineered Cas9 variants to develop five new base editors with distinct...