... CRISPR Plants CRISPR Plasmids: Plants Browse CRISPR Plasmids By Function...have been designed for use in plants. CRISPR...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes will introduce... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

...Rinehart Lab Phosphoprotein Reagents Rinehart Lab Phosphoprotein Reagents Protein phosphorylation is one... for Improved 2015 Phosphoprotein Reagents (DOCX, 525.9 KB) Reagents (ca. 2014) described in: Enhanced...Protocol for Reagents (ca. 2014) from the Rinehart lab: Users Guide for 2014 Phosphoprotein Reagents (PDF, 691.3...the Rinehart and Issacs labs at Yale have made improvements to this system by genomically recoding E.coli...translation system (pSerOTS-C1* (V70) 188537 ). These reagents are described in detail in (Mohler et al., Mol...for laboratories performing phosphoproteomic experiments in need of reference spectra or phosphopeptide...phosphopeptide standards for quantitative assessments. The mode #1 phosphosite library can be generated with either...

...describing these reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays...OPEN) Reagents - Engineer zinc finger arrays via OPEN method Modular Assembly Engineering Reagents - Engineer...Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium... Zinc Finger Consortium Zinc Finger Consortium Reagents You may also like... Keith Joung Lab plasmids ...Daniel Voytas have deposited at Addgene various reagents for engineering and expressing zinc finger proteins...Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

...function in zebrafish, plants, and human cells... Kits Zinc Finger Consortium OPEN Reagents Zinc Finger Consortium...Consortium: OPEN Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids...plasmids Detailed Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool ... request from the Joung lab) and the following reagents (listed below and available individually or as...as an “OPEN Accessory Reagent Set”). These reagents are also valuable research tools for using the bacterial...used to engineer ZFNs that function in zebrafish, plants, and human cells ( Maeder et al., Mol Cell 2008...

...consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end ...additional pages describing Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression plasmids (AsCpf1 and LbCpf1) ...) for use in mammalian cells SpCas9 variants with no detectable off-target effects Modified PAM specifity...

...Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9...Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR,...specialized Cas proteins snip foreign DNA into small fragments approximately 20 bp in length and paste them into...These edits can include mutations seen in human patients, protein tags, or loxP/FRT sites, among others... High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very...Streptococcus , Streptomyces , and others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae...

...GRAB biosensors for neuropeptides VIP, NPY, CCK, NTS, UCN, SST, and CRF A tool kit of highly selective...Quantitative measurements of membrane charges A FRET sensor enables quantitative measurements of membrane...designed to specifically localize to cellular compartments or organelles. Addgene’s database includes many...that many of the articles linked below include variants with different binding affinities or negative-...imaging activity in neuronal populations and microcompartments. Nat Methods. 2019 Jul;16(7):649-657. Douglas...astrocytes (GCaMP6f), cytosolic Conditions and constraints for astrocyte calcium signaling in the hippocampal... Calcium Yellow protein calcium sensor jYCaMP1 variants (Constitutive or Cre-dependent) jYCaMP: an optimized...

...NTF4 neurotrophin 4 NT-4/5, NT4, NT5, NTF5 NTS neurotensin NMN-125, NN, NT, NT/N, NTS1 NUDT6 nudix (nucleoside...Antibodies produced by B cells activate immune components that also lead to the destruction of pathogens...

... made up of microtubules, actin filaments, and intermediate filaments. These structures give the cell ...GFP-cortactin Actin Filaments Cortactin EGFP Anna Huttenlocher 32907 pEGFP-rNFM Intermediate Filaments (neuronal...Intermediate Filaments (neuronal) Nefm EGFP Anthony Brown 11908 pEGFP-N1 alpha-actinin 1 Actin Filaments alpha-actinin...Actin Filaments Cortactin mCherry Christien Merrifield 36201 pLifeAct-mTurquoise2 Actin Filaments LifeAct...pLifeAct_mScarlet_N1 Actin Filaments LifeAct mScarlet Dorus Gadella 85055 pLifeAct_mScarlet-H_N1 Actin Filaments LifeAct ... Actin Filaments ITPKA mTurquoise2 Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin...mCherry-Lifeact-7 Actin Filaments LifeAct mCherry* Michael Davidson 54610 mEGFP-Lifeact-7 Actin Filaments LifeAct EGFP...

...antibody fragments consisting of two scFvs bound to a CH3 domain. Fab fragments - Fab fragments are created... called the antigen binding fragments (Fab). The tips of the Fab fragments contain highly variable regions...Affinity Reagents There are several alternatives to antibodies, ranging from antibody fragments (shown ...Structure Isotypes Antigen Specificity Other Affinity Reagents Handling Visualization Signal Amplification Application...Multiplex Assays Selection Validation Affinity reagents are molecular tools used in the lab to specifically...foreign bodies, are the most commonly used affinity reagents. Antibodies are Y-shaped proteins, naturally produced...interest is introduced to the animal, which then mounts an immune response to it. Once the immune response...

...down which reagents are appropriate for a given experiment. Make sure to check whether reagents are available...you are ready to start navigating the different reagents that are available for your particular experiment...genetic manipulations require different CRISPR components. Selecting a specific genetic manipulation can...dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective than...such as dCas9-VP64) gRNA(s) targeting promoter elements of target gene Many different activators exist...cells), transfection with standard transfection reagents may be sufficient to express the CRISPR machinery...(e.g. primary cells), viral delivery of CRISPR reagents may be more appropriate. In cases where off-target...

...in monocot and dicot plants - Yao-Guang Liu. Plasmids for prime editing in plants (including rice and ...Addgene's plant resources: Find plasmids for use in plants. Plasmid...tools that were specifically designed for use in plants. These resources include tools for research using...useful toolkits and plasmid collections for use in plants. The tables below highlight plasmids spanning a...selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples...- Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA...assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector with GRF4-GIF1...

... regulatory elements on gene expression. Empty backbones for inserting regulatory elements before or after...secreted and more stable, making time course experiments possible, but its short luminescence half-life...commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated...Conformationally destabilized Firefly luciferase mutants : A set of three sensors that are increasingly ...for split Firefly Luciferase complementation in plants. Bioluminescence Resonance Energy Transfer (BRET...Creating ORF-Firefly luciferase gene fusions in plants. Renilla luciferase under the control of a CMV ...white screening can be used for screening of transformants in cloning process. Lynne Postovit 119760 pSGDlucV3.0...

...multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements, and other Cas...scale edits by integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated...mechanism Engineered CRISPR systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated...mechanisms of CRISPR and some of the ways the CRISPR components are leveraged to achieve specific goals, such...the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout assay are simple. You’...Cut) Multiplex Genome Engineering Many CRISPR experiments involve editing more than one gene. In these ...RuvC and HNH, can be altered to reduce cleavage events to a single cut (nick) or no cuts. Cas9 nickase...

...antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or insect...size. These recombinant antibody fragments can be used in experiments such as immunoprecipitation and ...antibodies as Fab' fragments, isotype-switched chimeric antibodies, and Fc-silent mutants. Functional diversification...microscopy. Two commonly used plasmid-based antibody fragments are scFvs and single-domain antibodies (also known..., scientists often use plasmids encoding scFv fragments that bind to the GCN4 peptide, from the popular...the generation of chicken monoclonal antibody fragments by phage display. J Immunol Methods. 2000 Aug ...dimerisation and detection of recombinant antibody fragments in the form of scFvs A simple vector system to...

... Guide for more information about: Lentiviral components 2nd versus 3rd generation lentiviral systems ...feature of lentivirus used in research is that the components necessary to produce an infectious viral particle...called packaging and envelope plasmids) encode components of the viral capsid and envelope and are used...transfer plasmids along with additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making...that encodes genetic material that the researcher wants delivered to specific target cells. This genome ... See article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion... 11619 pLB 3rd Modification of pLL3.7; Genetic elements known to prevent epigenetic silencing were added...

...point in bacterial growth? Can I produce large amounts of this protein and study it in vitro ? These, ...resources required for the production of large amounts of protein or high copy number plasmids. Whether...fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein variant (YPet...fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein variant (CyPet... pCri System Various Plasmids T7/lacO and lacO variants that can be expressed in B. subtilis Various combinations...constituitive promoters and translation initiation elements that can be used to drive gene expression at a...Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium. Plasmids...

...for many years and lead to a major burden on the patients, their families and caretakers, and the health...general. The required care for these millions of patients results in billions of dollars spent each year...changes in behavior and emotion, uncontrolled movements, memory deficits, as well as difficulty speaking...after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases of Alzheimer's...plasmids expressing wild type or mutant ORFs, gene fragments, and gRNAs for genes linked to neurodegenerative...discovery and advance development of diagnostics and treatments for Alzheimer’s disease and related disorders...foundation offers curated information on tools and reagents (Link opens in a new window) such as huntingtin...

...The RESOLUTE mission is to develop data and reagents to boost the research focused on Solute carrier ...Union. The RESOLUTE mission is to develop data and reagents to boost the research focused on SLCs. Through...open-access ethos’ to the results, techniques and reagents with the highest-possible quality of research ...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...libraries are useful tools for genetic screening experiments. These libraries target the solute carrier family...feedback form . If you have further questions or comments you can also contact them at [email protected]...

...payment, and billing. Industry Material Transfer Agreements Most Addgene orders must be accompanied by a ...protection for material providers. Addgene’s deposit agreements allow Addgene to distribute plasmids on behalf...behalf of the depositing institutions. These agreements indicate the language which we are allowed to use...use for distribution. Please see the Sample Agreements on our Technology Transfer Information page for ...authorized signatory is someone who can execute legal documents on behalf of the recipient institution. Once the...addgene.org . After we review and approve the MTA documents, your order will be processed and shipped. Industry...