What is Polymerase Chain Reaction (PCR)
Type
Protocol
... Taq buffer with MgCl 2 1 μl dNTP mix (10 mM each nt) 2.5 μL Forward Primer (10 μM stock) 2.5 μL Reverse...total + 10% (3.5μl) = 38.5μl dNTP mix (10 mM each nt): 1µl x 7 reactions = 7µl total + 10% (0.7µl) = 7.7µl...