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We narrowed to 660 results for: otos

Showing: 241 - 260 of 660 results
  1. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual...Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence Derivative Sources...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...
  2. Reflections and Looking Ahead

    Type
    Blog Post
    ...Gonzalez Bolivar, S., Alende, C., Ruiz Moleon, V., Fotouhi, M., Alqazzaz, M., Southern, K., Alshafie, W., ...platform for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn...
  3. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...gene results in the degeneration of spinal cord motor neurons. This disease is estimated to affect anywhere...molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy patients...
  4. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...integration into the array, these sequences are called protospacers. Repeats: Bacterial DNA that appears between...sequence matches the guide sequence. PAM: The “protospacer-adjacent motif” present in the non-target strand...
  5. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...in reinforcement learning, decision making, and motor control.  What do we understand about Dopamine?...Learn about Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research...
  6. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... the most accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase by Outreach...Addgene founder, Melina Fan, recently shared our protocol for making competent cells to save time and money...
  7. Bacterial Expression Systems

    Type
    Collection
    ...collection of FPs, including photoactivatable, photoconvertible, and photoswitchable proteins, organized by ...Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers...Inducible Promoters Repressible Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter...
  8. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...endogenous IgGs in your tissue sample. Most common IHC protocols use two antibodies - one against your target of...… Another strategy for species-on-species IHC protocols is to block endogenous IgGs by pre-treating the...
  9. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative...General Use Antibodies Antibody guide Antibody protocols Additional resources on the Addgene blog Antibodies...
  10. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...recombinant antibodies, educational materials, protocols, and shared data, on our values of transparency... antibody collection.  Addgene is sharing our protocols for recombinant antibody production as well as...
  11. RUBY-Red Siliques

    Type
    Blog Post
    ... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby.   Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby. The ease of observing...
  12. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...
Showing: 241 - 260 of 660 results