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We narrowed to 14 results for: otos

Showing: 1 - 14 of 14 results

  1. Optogenetics Guide

    Type
    Guide
    ...reduces photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/...bacterial photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein... 400 UVR8 domains UVR8 plant photoreceptor protein that forms photolabile homodimers with slow reversal...responses, such as nonspecific cation influx vs. proton efflux. Researchers have used genetic engineering...neuron. Feature enhancements include: Increased photocurrent amplitude Examples: ChR2(H134R), C1V1(t/t), ...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...viral titer. For more detailed protocols, see Addgene’s viral vector protocols . Production Using Packaging...information in Addgene’s stable cell line protocol . Addgene’s protocol is for lentiviral vectors, but a similar...Addgene protocols Generating Stable Cell Lines Using Lentiviral Vectors Addgene's Viral Protocols References...enhancers. Many times, integration sites are found in proto-oncogenes. This can lead to activation of the oncogene... offer the benefit of having well-established protocols for fast and efficient generation of large-scale...carry a slightly elevated risk of integrating into proto-oncogenes. As with most experiments, infection risks...., Nikoniuk, A., Macmorland, W., Horlock, C., Matsumoto, S., Williams, S., Smith, K., Price, J., Srivastava...

  3. CRISPR Guide

    Type
    Guide
    ...edition) CRISPR software and depositor protocols Video protocol for genomic deletions in mammalian cell...target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...before use (Figure 8C), following the amplification protocol specified by the depositing lab. After amplification...The translated codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target...engineering using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A....

  4. Educational Resources

    Type
    Guide
    ...including protocols, tips on using Addgene’s materials, and career advice. Playlists include: Protocols Careers... Molecular Cloning Techniques Protocols Find written and video protocols for a wide range of applications...videos, and protocols Educational... Educational Resources From eBooks to videos and blog posts to protocols, our educational...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...transgene. For more detailed protocols, see Addgene’s viral vector protocols . Figure 5: Overview of lentiviral...Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector Production...vectors can be found in Addgene’s stable cell line protocol . Browse Addgene's collection of in-stock lentiviral...Lines Using Lentiviral Vectors Addgene's Viral Protocols References Cronin, J., Zhang, X., & Reiser, J....mapping of lentiviral integration sites. Nature Protocols , 14 (1), 153–170. https://doi.org/10.1038/s41596...

  6. Plan Your Experiment

    Type
    Guide
    ...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...10.1038/s41587-020-0490-7 PMID: 29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the...used as positive controls when working out a new protocol. Read more about how to design your gRNA . Synthesize...editing experiments in mammalian cells. Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/...

  7. Chemogenetics Guide

    Type
    Guide
    ...PSAM-5HT3 LGICs pair a PSAM domain with a 5-HT 3 serotonin receptor sodium- or potassium-selective IPD. Binding...molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID: 38450294 (Link opens in a...DiBerto JF, Xiong Y, Urushihata T, Hirabayashi T, Fujimoto A, Mimura K, English JG, Liu J, Inoue K, Kumata...Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T (2020). Deschloroclozapine, a potent and selective...luminopsins with improved coupling efficiencies. Neurophotonics. 11:024208. PMID: 38559366 (Link opens in a...

  8. Cloning

    Type
    Guide
    ... a LIC protocol on our website. Ligation Independent Cloning (Figure adapted from LIC protocol ) Back ...compared to other cloning methods. You can find a protocol for restriction cloning and an in-depth breakdown...cloning, more commonly known as Gibson assembly ( protocol ), takes advantage of the properties of 3 common...

  9. Modular Cloning Guide

    Type
    Guide
    ...collection of 93 modular plasmids and enhanced MoClo protocols for rapid one-pot, multipart assembly, combinatorial...Engineering, CRISPR, Mammalian Expression Takashi Yamamoto Plasmids for construction of all-in-one CRISPR...Accessory Pack Genome Engineering, TALEN Takashi Yamamoto Nine pFUS array vectors and destination vectors...Gate TALEN Kit Genome Engineering, TALEN Takashi Yamamoto Plasmids for construction of highly-active Platinum...

  10. Molecular Biology Reference

    Type
    Guide
    ...dedicated to lab protocols and videos for specific techniques and tips! Addgene Protocols Addgene Video ...Resources Genetic Code Webpage and Blog References Protocols Origins of Molecular Genetics The concept of genes...Power Behind NGS Plasmid Verification: seqWell Protocols There are many different lab techniques that are...

  11. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... models. For more detailed protocols, see Addgene’s Viral Vector Protocols . Figure 3: Overview of rAAV...Liver AAV8, AAV9 Lung AAV6 Pancreas AAV2, AAV8 Photoreceptor cells AAV2, AAV5, AAV8 Skeletal muscle AAV1,...Vectors 101 topics More AAV Vector topics Addgene Protocols General Transfection AAV Production in HEK293T...

  12. Antibody Guide

    Type
    Guide
    ...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification Methods...binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication. This...Special considerations Many of the steps in this protocol vary depending on assay and microscopy techniques... may be a fluorophore or an enzyme. The basic protocols and controls follow that of IF (above). Sample...

  13. Guide to Using Pooled Libraries

    Type
    Guide
    ...Library Amplification Protocol for Addgene's generalized library amplification protocol. Read our CRISPR guide...amplify the library, please refer to the depositor’s protocol for the best results. For some libraries, plasmid...

  14. Adenovirus Guide

    Type
    Guide
    ..., K. W., Vogelstein, B., & He, T. C. (2007). A protocol for rapid generation of recombinant adenoviruses...adenoviruses using the AdEasy system . Nature Protocols, 2 (5), 1236–1247. https://doi.org/10.1038/nprot.2007.135...17546019 (Link opens in a new window) Matsunaga, W., & Gotoh, A. (2023). Adenovirus as a vector and oncolytic...
Showing: 1 - 14 of 14 results