...reduces photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/...bacterial photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein... 400 UVR8 domains UVR8 plant photoreceptor protein that forms photolabile homodimers with slow reversal...responses, such as nonspecific cation influx vs. proton efflux. Researchers have used genetic engineering...neuron. Feature enhancements include: Increased photocurrent amplitude Examples: ChR2(H134R), C1V1(t/t), ...

...) CRISPR software and depositor protocols JOVE video reprint: Protocol for genomic deletions in mammalian...target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...before use (Figure 8C), following the amplification protocol specified by the depositing lab. After amplification...The translated codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target...engineering using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A....

...including protocols, tips on using Addgene’s materials, and career advice. Playlists include: Protocols Careers... Molecular Cloning Techniques Protocols Find written and video protocols for a wide range of applications...videos, and protocols Educational... Educational Resources From eBooks to videos and blog posts to protocols, our educational...

...Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic deletions...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...Biology . 1239 , 197–217. PMID: 25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the...

...PSAM-5HT3 LGICs pair a PSAM domain with a 5-HT 3 serotonin receptor sodium- or potassium-selective IPD. Binding...molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID: 38450294 (Link opens in a...DiBerto JF, Xiong Y, Urushihata T, Hirabayashi T, Fujimoto A, Mimura K, English JG, Liu J, Inoue K, Kumata...Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T (2020). Deschloroclozapine, a potent and selective...luminopsins with improved coupling efficiencies. Neurophotonics. 11:024208. PMID: 38559366 (Link opens in a...

... a LIC protocol on our website. Ligation Independent Cloning (Figure adapted from LIC protocol ) Back ...compared to other cloning methods. You can find a protocol for restriction cloning and an in-depth breakdown...cloning, more commonly known as Gibson assembly ( protocol ), takes advantage of the properties of 3 common...

... details, see the (Link opens in a new window) protocol available at the (Link opens in a new window) ...changing a MCS, visit Addgene's Plasmid Reference and Protocol Guide . How do I clone my insert into a transfer...through Addgene's viral service Read our detailed protocol for using the popular cloning vector pLKO.1. Trono...Maps Standard Lentiviral Production & Titration Protocols Publications Retroviral integration site selection...

...dedicated to lab protocols and videos for specific techniques and tips! Addgene Protocols Addgene Video ...Resources Genetic Code Webpage and Blog References Protocols Origins of Molecular Genetics The concept of genes...Power Behind NGS Plasmid Verification: seqWell Protocols There are many different lab techniques that are...

...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification Methods...binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication. This...Special considerations Many of the steps in this protocol vary depending on assay and microscopy techniques... may be a fluorophore or an enzyme. The basic protocols and controls follow that of IF (above). Sample...

...Library Amplification Protocol for Addgene's generalized library amplification protocol. Read our CRISPR guide...amplify the library, please refer to the depositor’s protocol for the best results. For some libraries, plasmid...

...such as adenovirus or herpes simplex virus, or a genotoxic agent such as UV radiation or hydroxyurea, is ... AAV4, AAV5, AAV6, AAV9 Pancreas AAV6, AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment ...