We narrowed to 499 results for: plasmid dna

...genome is lethal. If this DSB occurs on a plasmid, the plasmid will be eliminated from the bacterium, which...sequence-specific, antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here). In both cases, ...genes in E. coli using plasmid and phagemid delivery systems. Phagemids are plasmids containing a phage origin...sequence results in cell death. Plasmid targeting may result in plasmid loss and antibiotic resensitization... three essential processes: cell wall synthesis, DNA replication, or protein synthesis. Because they target...this resistance gene being encoded by a high-copy plasmid. In the case of the genomically encoded quinolone...resistance, mediated by a single base pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for ...

... awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...Learn about the Addgene, Seeding Labs Plasmid Grant Read about Plasmid Grant Recipient, Gladys Kaba Resources... fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby...accelerate their research. Kwabena came across the plasmid grant in Seeding Lab’s Newsletter and recently ...plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian... Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher...the University of Edinburgh - synthetic biology, DNA assembly methods, and engineering cellulose degradation...

...exact Cas9 plasmid used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and... hand, generates staggered DNA nicks at its target in cis and cleaves ssDNA in trans. In sum, CasPEDIA...CasPEDIA documents the target substrate (DNA/RNA and strandedness) along with its action on the substrate (nick...find a link to search Addgene's collection for plasmids containing that enzyme, and embedded in the References...lists on every page you can find direct links to plasmids from the article if they're available through ...problem: CasPEDIA, a new resource from Jennifer Doudna’s lab at the Innovative Genomics Institute. What...frequently used Cas enzymes) cleaves both strands of its dsDNA target in cis and has no trans activity. AsCas12a...

...working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare diseases ...Richard Gronostajski who each are being awarded free plasmids form Addgene to accelerate their research. Read... on to learn how these researchers plan to use plasmids from Addgene to advance their research on diseases...Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune cell...understanding the structural determinants of protein-DNA interactions and how these interactions regulation...Nfix-negative neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors...

... entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed ...Addgene. Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based...SARS-CoV-2 Plasmids for Many Expression Systems Collection: COVID-19 and Coronavirus Plasmids & Resources...Browse Ginkgo Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems. Depositor...Ginkgo Bioworks COVID-19 Ginkgo Bioworks COVID-19 Plasmid Collection (Link opens in a new window) Researchers...therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for many ...Bioworks (Link opens in a new window) used their DNA design and synthesis capabilities, in collaboration...

...CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces time to generate...repair templates with homology arms of 35-80 bp, and DNA double-strand breaks up to 54 bp from the desired...in knock-in efficiency, presumably by channeling DNA breaks into the homologous recombination repair pathway.... elegans. These modular toolkits build single plasmid targeting vectors that encode the gRNA transcript... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans. Genetics 210:781...5′ Modifications Improve Potency and Efficacy of DNA Donors for Precision Genome Editing. BioRxiv. https...

...biotinylating proteins with BirA. You can find plasmids that contain both AviTag and BirA at Addgene! ...labeling Biotin can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR)...Photobiotin can be used to non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin...electrophoretic mobility shift assay to examine specific dsDNA, ssDNA or RNA–protein interactions.” Nucleic Acids ...

...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR...protein (dCas9) is well known for its ability to bind DNA targets without changing them. Thus, it has been ...cells. Based upon this, they chose an intergenic DNA region (IDR3) as a reference loci to measure inter-locus..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...

... target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP target plasmid contains...pCAG-EGXXFP plasmid along with the individual gRNAs in their PX330 plasmids (or similar plasmids for gRNA... (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences ...degradation that can set you back days. Working with plasmid DNA is much easier and doesn’t require the extra ... system floating around, and many pre-designed plasmids containing these variants ready for purchase. ... or you can order them yourself through whatever DNA supply company you currently use. You can also find...multi-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized Cas9 expression cassette and...

...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...indels after DNA repair. B) M. abscessus is first transformed with Cas9 on an integrative plasmid containing... or want to swap out the constant domains, the plasmids for the heavy chain and light chain are also available...ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova, A. et al. (2024). Design of ...minimal background.   Find these C. elegans mScarlet plasmids here!   Cao W.X., Merritt D.M., Pe K., Cesar ...disruption in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system By Aryana Khosravani Deborah...Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium...

...some time now. When titrating AAV by qPCR, viral DNA is amplified and monitored in real time. When the...comparing the threshold fluorescence of the viral DNA to the threshold fluorescence of a standard - a set... set of reactions with known quantities of DNA. Additionally, an AAV reference, a virus with a known titer...get right. Here at Addgene, we use a linearized plasmid standard. However, even when the standard is made... the ingredients for amplification of the target DNA. This partitioning reduces the number of PCR inhibitors... circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine evaluation in ...

... projects, consider making you own DNA ladders with these plasmids from Penn State Need help picking the...are produced in animals instead of via recombinant DNA, they can’t be genetically tagged and instead are... a reporter and a nanobody that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...

...cared for your packaging cell line, prepped your DNA, transfected and harvested your lentivirus. Now it...for expression of a gene carried on the transfer plasmid or quantifying the number of viral copies that ...beneficial as they ensure that the correct transfer plasmid was used in the transfection; if preparing several... that comes from amplification of the original plasmid which can over-inflate results. A packing vector...antibiotic resistance genes carried on transfer plasmids. Target cells are transduced with serial dilutions...transduced with serial dilutions of a viral prep, genomic DNA isolated, and primers targetting viral components...In this approach, viral RNA is first converted to cDNA and then quantified using qPCR primers targeting...

...function in cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor...mTOR pathway Ras pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription...tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation... negative regulators, increase its stability and DNA binding activity, and allow it to bind transcriptional...of these mutations are missense mutations in the DNA-binding domain, often at one of six hotspot residues...

...scientific community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...days, we would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while...while more recently we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation... to a partnership with Seqera Labs.              DNA sequencing drives our bioinformatics needs   ...lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection...transform the raw sequencing data into a complete plasmid sequence that would be ready for our QC team to...With our outline of individual steps to automate plasmid sequence assembly, and possible solutions for each...

...tetracycline resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI...engineering, cloning, and plasmid tags, addtional content added to the "Plasmids that Glow" and "Viral Expression...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua...Lederberg's Personal Perspective: Plasmid (1952-1997) Cohen, Stanley N. “DNA Cloning: A Personal View after...factors played within an organism.  So how did plasmids get their name? In 1952, Joshua Lederberg set...inheritance factors. He proposed the catch-all term “plasmid” derived as a hybrid of "cytoplasm" and "id" (Latin...” was eventually dropped and we’ve been using "plasmid" ever since! From napkins to notebooks Although...

... 1998). Interested in plasmids that contain the groucho gene? Check out plasmids deposited by the Shivdasani... several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid however was... to see for yourself. We have one plasmid in our repository, plasmid 39624, that contains cmr.   tinman...website for plasmids that contain tinman or the mammalian homolog Nkx2.5. We also have one plasmid, Addgene...Drosophila Read our Plasmids 101 blog series Resources on Addgene.org Find plasmids for your research ...the stories behind the names. We also have many plasmids in our repository that you can use to study these..., and even humans. It binds to a specific set of DNA-binding transcription factors to repress the expression...

...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...more than just the DNA sequence. If this is the case, you can confirm your edit using DNA-based methods like...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...mutations or small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ...efficiency . Remember that HDR also requires donor DNA to serve as template for the repair. Once you settle...settle on your gRNA, you can design a donor DNA to have the appropriate left and right homology arms. See...

...bacterial in nature. All three of these methods involve DNA incorporation into the cell, but the mechanisms vary...lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector that...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...

...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...of the viral genome and the absence of unexpected DNA contaminants. The data is also used to determine ...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting integrated...