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...allows both insert and vector to be digested in the same tube. Alternatively, SLIC can also work directly...using multiple fragments that share homology in the same reaction. In these cases, the use of another cloning...

...cis-acting elements, which means that they are in the same DNA molecule as the gene that they package. In a.... M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific...

...running it unchanged over and over again, and the same goes for your workflow. Next time you make a mistake... to match your plate layout can help you track samples as you add them. Moving your reagents from your...

... the antibodies time-sensitive, using precious samples, or being compared to other data in a manner that...signaling molecule, that all need to be detected in the same assay. This is one of the most complex subjects ...

...defined disease phenotype. These screens employ the same downstream manipulations as a forward or reverse...unlike in screens. Selections begin and end with the same steps as screens – they start with manipulations...

...that helps visually impaired readers receive the same information as sighted readers.    Figure...Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of them had...

...Single Nucleus from a Set of Mitotic Chromosomes. Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude...

...recombination so that the transgenes can be inherited on the same chromosome. Targeted Insertion allows for direct...variants of YFG because the transgenes are found in the same locus across fly lines (i.e. there won’t be positioning...

...2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally...

...insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly and GeneArt cloning...plasmid. For insertions, it’s possible to use the same technique just using a large primer known as a megaprimer...

...Allows all group members to lead and learn at the same time Broadens the network of the participants Can...managing to get together. If you all work at the same place, a 1-2 hour lunch on a regular schedule might...

...of individuals and find several mutations in the same gene or in multiple genes, all of which seem to ...insert all of those variants into stem cells with the same genetic background and do an apples-to-apples comparison...

...Because we have a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth ...quality reagents, and we realized we could use the same principles to provide recombinant antibodies, another...

...episomal and does not divide and replicate in the same way the host DNA does. Because of this, fast dividing...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...

...overall inflation of USD has gone up 19.8% in the same time period. For ease of calculations*, let’s split...inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents and consumables...

...systems can lead to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment...dsRNA finds its way outside of the mitochondria, the same inflammatory response can activate. These types ...

...protein will be expressed in the same cell, but not necessarily at the same subcellular compartment as the...

...isolated which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking...Cas13 and has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.)....

...communication. Researchers often post a preprint at the same time as, or before, submitting the manuscript to...plasmids that were used in a preprint provides all the same benefits as sharing those plasmids alongside a journal...

...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested at its optimum...