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We narrowed to 601 results for: sam

Showing: 241 - 260 of 601 results
  1. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally...
  2. Plasmids 101: Screens vs. Selections

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    Blog Post
    ...defined disease phenotype. These screens employ the same downstream manipulations as a forward or reverse...unlike in screens. Selections begin and end with the same steps as screens – they start with manipulations...
  3. Sweating the Small Stuff: Details in the Lab

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    Blog Post
    ...running it unchanged over and over again, and the same goes for your workflow. Next time you make a mistake... to match your plate layout can help you track samples as you add them. Moving your reagents from your...
  4. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post
    ... the antibodies time-sensitive, using precious samples, or being compared to other data in a manner that...signaling molecule, that all need to be detected in the same assay. This is one of the most complex subjects ...
  5. Clear and Accessible Writing

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    Blog Post
    ...that helps visually impaired readers receive the same information as sighted readers.    Figure...Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of them had...
  6. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post
    ...cis-acting elements, which means that they are in the same DNA molecule as the gene that they package. In a.... M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific...
  7. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post
    ...of individuals and find several mutations in the same gene or in multiple genes, all of which seem to ...insert all of those variants into stem cells with the same genetic background and do an apples-to-apples comparison...
  8. RaPID Detection of RNA-protein Interactions

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    Blog Post
    ...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested at its optimum...
  9. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post
    ...hypodermis and gut cells of C. elegans causes the same phenotype. Activation of the Raf-MEK-ERK signaling...signaling pathway with psRaf1 or psMEK1 in these same cells by 24-48 hours exposure to blue light also induced...
  10. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post
    ...isolated which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking...Cas13 and has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.)....
  11. Summer SciComm: Preprints

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    Blog Post
    ...communication. Researchers often post a preprint at the same time as, or before, submitting the manuscript to...plasmids that were used in a preprint provides all the same benefits as sharing those plasmids alongside a journal...
  12. Addgene: The First Twenty Years

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    Blog Post
    ...Because we have a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth ...quality reagents, and we realized we could use the same principles to provide recombinant antibodies, another...
  13. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ...episomal and does not divide and replicate in the same way the host DNA does. Because of this, fast dividing...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...
  14. The time and cost required to make a plasmid

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    Blog Post
    ...overall inflation of USD has gone up 19.8% in the same time period. For ease of calculations*, let’s split...inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents and consumables...
  15. All in a Twist: dsRNA

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    Blog Post
    ...systems can lead to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment...dsRNA finds its way outside of the mitochondria, the same inflammatory response can activate. These types ...
Showing: 241 - 260 of 601 results