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We narrowed to 610 results for: sam

Showing: 241 - 260 of 610 results

  2. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ... over.” And we sent the Addgenies home.  At the same time, as Addgene started dealing with this new reality...and keep new projects on track to succeed at the same time. I began approaching science funding organizations...

  3. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...fragments containing the desired mutations. This same process can be used simultaneously for the generation...cassette, ready for transformation into yeast that same day.  The field of synthetic biology has led to ...

  4. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...fitting that I’ve ended up (again) dispelling the same myths and misconceptions fifteen years later. What..., peer-reviewed study. The second group read the same abstract but with the names reversed, meaning that...

  5. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ... Attend events for one organization or go to the same conferences/meetings over and over to get to know...volunteer). Naturally running into or working with the same people is often the easiest way to build relationships...

  6. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...libraries, but any analysis script should return the same output: a spreadsheet of read counts. You can use...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...use of the two systems in different tissues of the same animal. Flipbow additionally incorporates SUMO tags...which express self-excising Cre recombinase from the same transgene as the Brainbow cassette, simplifying ...expression plasmids for a given species are based on the same backbone to permit expression levels to be directly...

  10. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally...

  12. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...running it unchanged over and over again, and the same goes for your workflow. Next time you make a mistake... to match your plate layout can help you track samples as you add them. Moving your reagents from your...

  13. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... the antibodies time-sensitive, using precious samples, or being compared to other data in a manner that...signaling molecule, that all need to be detected in the same assay. This is one of the most complex subjects ...

  14. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...defined disease phenotype. These screens employ the same downstream manipulations as a forward or reverse...unlike in screens. Selections begin and end with the same steps as screens – they start with manipulations...

  15. Clear and Accessible Writing

    Type
    Blog Post
    ...that helps visually impaired readers receive the same information as sighted readers.    Figure...Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of them had...

  16. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...cis-acting elements, which means that they are in the same DNA molecule as the gene that they package. In a.... M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific...

  19. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...of individuals and find several mutations in the same gene or in multiple genes, all of which seem to ...insert all of those variants into stem cells with the same genetic background and do an apples-to-apples comparison...

  20. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Because we have a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth ...quality reagents, and we realized we could use the same principles to provide recombinant antibodies, another...
Showing: 241 - 260 of 610 results