We narrowed to 333 results for: uros

1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

   ...vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell clone selection. For more...

2. Fluorescent Proteins 101: Introduction to FRET

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   Blog Post

   ...molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find...

3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...using CRISPR–dCas9-activator transgenic mice. Nat Neurosci 21:440–446 . https://doi.org/10.1038/s41593-017...

4. Adapting Toehold Switches to Detect Zika Virus

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   Blog Post

   ...important in the current outbreak because of the neurological symptoms associated with the American-lineage...

5. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ...parents' and grandparents' slow growth period." European Journal of Human Genetics 10.11 (2002): 682-688...

6. Experimenting in an Open Source lab: from CRISPR to Cats

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   Blog Post

   ...final target decisions, we intend to focus on motor neuron knockouts so that we can perturb factors related...

7. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   .... (2002). Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...

8. Viral Vectors 101: Producing Your rAAV

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   ... of gene expression in the CNS. Molecular Neurodegeneration, 15(1), Article 1. https://doi.org/10.1186...

9. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   Blog Post

   ... https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT...

10. Of Myc and Men

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    Blog Post

    ...tagging experiment involved a peptide from the neuropeptide substance P. This protein had a highly specific...

11. Plasmids 101: Multicistronic Vectors

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    ...pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian ...

12. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ...spanning chemical biology, biochemistry, and neurobiology. CBE Life Sciences Education. 15(4):ar55. Ma...

13. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post

    ...metastasized tumor cells, or alzheimers-affected neurons, or [your cell line of interest], is the foundation...

14. Lentiviral Vector Uses and Overview

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    Blog Post

    ...experimental use. In 1996, Naldini et al. transduced rat neurons in vivo with beta-gal and other reporters, and ...

15. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ... to reduce false negatives and produce a more vigourous screen is to use a number of different bait and...

16. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ...aware that  “organizing the fun” sounds a little neurotic, but hey- sometimes fun needs to be had in an ...

17. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...the US (New York University School of Medicine), Europe (Basel Institute for Immunology), and Japan (University...

18. Retrovirus Plasmids

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    Collection

    ... expression; also expresses GFP. See pMKO.1 puro for Puro resistance. Hahn 63704 pRetroX GFP T2A Cre CMV...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen... immortalized cells; additional plasmids include Puro resistance and Neo resistance Weinberg 10676 pMKO...CMV/MSV pRXTN is a modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 MSCV-... for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression plasmid; deletion...

19. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces naeslundii, A. viscosus, Aerobacter...

20. Hot Plasmids - August 2020

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    Blog Post

    ...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...