... when mismatches occur near the PAM sequence, so gRNAs with no homology or those with mismatches close...on-target cleavage. The gRNA target sequence needs to match the target locus, but it is also critical to ensure...ensure that the gRNA target sequence does NOT match additional sites within the genome. In a perfect world...at the same position in spite of being a perfect match for the target sequence. Many gRNA design programs...your desired edit, including but not limited to: Mismatch-cleavage assay (for NHEJ repaired DSBs): Provides...

...these common antibody applications.  Thanks for watching, and we hope you found the video radd-ically helpful...

...shared on our @addgene Instagram account, or you can watch the full animation on our YouTube channel.  More...

... Data Science and head of our Data Team, “Our patchwork collection of scripts evolved into a single, organized...

...make the decision between some meaningful names…. Watch the video to find out the final four! (Note: For...

...holiday cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis ...

...Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et....

...for that too.   This could be you after watching Addgene’s protocol videos. via GIPHY   If ...

... repository See blog post Biomed Resource Watch Any Any Search engine May include validation...

...it next to our lab trash (which coincidentally matches our Christmas tree's RGB colors) 😅🎅🤶🎄👨‍🔬👩‍...